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Lower Cape Fear River Program 2018 reportEnvironmental Assessment of the Lower Cape Fear River System, 2018 By Michael A. Mallin, Matthew R. McIver and James F. Merritt August 2019 CMS Report No. 19-02 Center for Marine Science University of North Carolina Wilmington Wilmington, N.C. 28409 Executive Summary Background – Multi-parameter water quality sampling for the Lower Cape Fear River Program (LCFRP) http://www.uncw.edu/cms/aelab/LCFRP/index.htm, has been ongoing since June 1995. Scientists from the University of North Carolina Wilmington’s (UNCW) Aquatic Ecology Laboratory perform the sampling effort. The LCFRP currently encompasses 32 water sampling stations throughout the lower Cape Fear, Black, and Northeast Cape Fear River watersheds. The LCFRP sampling program includes physical, chemical, and biological water quality measurements and analyses of the benthic and epibenthic macroinvertebrate communities, and has in the past included assessment of the fish communities. Principal conclusions of the UNCW researchers conducting these analyses are presented below, with emphasis on water quality of the period January - December 2018. The opinions expressed are those of UNCW scientists and do not necessarily reflect viewpoints of individual contributors to the Lower Cape Fear River Program. The mainstem lower Cape Fear River is a 6th order stream characterized by periodically turbid water containing moderate to high levels of inorganic nutrients. It is fed by two large 5th order blackwater rivers (the Black and Northeast Cape Fear Rivers) that have low levels of turbidity, but highly colored water with less inorganic nutrient content than the mainstem. While nutrients are reasonably high in the river channels, major algal blooms are normally rare because light is attenuated by water color or turbidity, and flushing in the estuary is usually high (Ensign et al. 2004). During periods of low flow algal biomass as chlorophyll a increases in the cape Fear River because lower flow causes settling of more solids and improves light conditions for algal growth. Periodically major algal blooms are seen in the tributary stream stations, some of which are impacted by point source discharges. Below some point sources, nutrient loading can be high and fecal coliform contamination occurs. Other stream stations drain blackwater swamps or agricultural areas (traditional agriculture and/or industrialized animal production), and some sites periodically show elevated pollutant loads or effects (Mallin et al. 2001). This region has been hit by hurricanes several times in the past three decades and such storms have a marked impact on water quality and organisms. GenX Issues - During the past three years there has been considerable controversy in the lower Cape Fear River watershed regarding a family of manufactured chemical compounds collectively known as GenX. To briefly summarize, DuPont constructed a facility known as Fayetteville Works near the river downstream of Fayetteville, where it manufactured fluoropolymers since 1971. DuPont manufactured a chemical called PFOA at Fayetteville Works beginning in 2001, then later stopped its manufacture due to health concerns surrounding this chemical. They then developed a substitute chemical called GenX, which they began manufacturing there, along with GenX’s parent compound, called HFPO-DA fluoride. Both compounds hydrolize in water to a third compound called HFPO-DA, CAS; the toxicity of this group of chemicals is unclear. Subsequently, DuPont spun-off a company called Chemours, which assumed plant operations in 2015. In the past few years researchers from US EPA, North Carolina State University, and the University of North Carolina Wilmington have found HFPO-DA 1 and related fluoroethers (which tend to be lumped under the blanket term GenX) in river water, river sediments, well water near the plant, aquatic organisms, in air samples, and in finished drinking water at the Wilmington water treatment facility, which obtains its water near Lock and Dam #1. Fayetteville Works is currently trucking their wastewater out of state for treatment, and lawsuits have been filed against the company from NCDEQ and Cape Fear River Watch to cease releases and provide financial compensation. Sampling and analysis of GenX and related compounds is outside of the purview of the scientific staff of the Lower Cape Fear River Program and will not be discussed in this report. Summary of water quality data results from 2018 – The major single impact of 2018 was the arrival of Hurricane Florence, which dumped approximately two feet of water on the region in September 2018 (Plate 1). Biochemical oxygen-demanding materials from industrial livestock production facilities, wastewater treatment plants, flooded septic systems and natural debris caused very low dissolved oxygen (DO) concentrations in all major rivers, but especially in the Northeast Cape Fear River, where DO dropped down to 0.2 mg/L for weeks at the two LCFRP sites. Massive fish kills occurred in that river as well as the mainstem Cape Fear River (Plate 1). We note, however, that even before the storm DO concentrations were very low in some areas, often falling below 4.0 mg/L at several blackwater and upper estuary stations. Year after year there is a dissolved oxygen sag in the main river channel that begins at Station DP below a paper mill discharge and near the Black River input, and persists into the mesohaline portion of the estuary. Mean oxygen levels are highest at the upper river stations NC11 and ANC and in the low-to-middle estuary at stations M35 to M18. Lowest mainstem mean DO levels normally occur at the river and upper estuary stations NAV, HB, BRR and M61. The Northeast Cape Fear and Black Rivers are classified as blackwater systems because of their tea colored water. The Northeast Cape Fear and Black Rivers generally have lower DO levels than the mainstem Cape Fear River. Dissolved oxygen concentrations in the tributary streams were briefly impacted by the hurricane, but some are chronically bad year-after-year. In 2018 SC-CH and GS were below standard 27% of the time sampled, and NC403 and AC below standard 18% of the time. Considering all sites sampled in 2018, we rated 28% as poor for dissolved oxygen, 16% as fair, and 56% as good. Annual mean turbidity levels for 2018 were lower than the long-term average in all estuary stations. Highest mean riverine turbidities were at NC11-DP (26-19 NTU) with turbidities generally low in the middle to lower estuary. The estuarine stations did not exceed the estuarine turbidity standard on our sampling trips. Turbidity was considerably lower in the Northeast Cape Fear River and Black River than in the mainstem river. Turbidity levels were low in the freshwater streams, with all streams rated as good for 2018. 2 Average chlorophyll a concentrations across most sites were low in 2018. The standard of 40 µg/L was exceeded once at Station SR, and there were several smaller algal blooms as well. We note the highest chlorophyll a levels in the river and estuary typically occur late spring to late-summer. High river discharge prevents riverine algal blooms from forming. For 2018, discharge at Lock and Dam #1 in the May-September growing season (8,590 CFS) was 4X the 2009-2012 low-flow period average of 1,698 CFS. Nuisance cyanobacterial blooms did not occur in the river and upper estuary in 2018, probably due to the elevated discharge washing out any algal bloom formation. For the 2018 period UNCW rated 97% of the stations as good in terms of chlorophyll a. Fecal bacteria counts in the estuary and at many of the stream stations were elevated in 2018. Almost all of the stream stations in the Northeast Cape Fear and Black River basins were rated as poor for fecal coliform bacteria counts. However, the main river and estuary sites were generally in good condition in 2018. For bacterial water quality overall, 39% of the sites rated as poor, 22% as fair, and 39% as good in 2018. In addition, by our UNCW standards excessive nitrate and phosphorus concentrations were problematic at a number of stations. Plate 1. Hurricane Florence high water marks along the Cape Fear River and Harrison’s Creek, dead fish at Lock and Dam #1, flooded poultry and swine lagoons (CAFO photos W.Golder, Audubon), and dead fish at Northeast Cape Fear River boat ramp (photos M.Mallin unless noted otherwise). 3 Table of Contents Executive Summary…………………………………………………………………………….1 1.0 Introduction...........................................................................………...............…........5 1.1 Site Description................................................………....................................6 1.2 Report Organization………………………………………………………..………7 2.0 Physical, Chemical, and Biological Characteristics of the Lower Cape Fear River and Estuary………………………………………………..……………………..............11 Physical Parameters..…......................………..........................................……....14 Chemical Parameters…....……..……….........................................................…..18 Biological Parameters.......……….....……......................................................…..21 4 1.0 Introduction Michael A. Mallin Aquatic Ecology Laboratory Center for Marine Science University of North Carolina Wilmington The Lower Cape Fear River Program is a unique science and education program that has a mission to develop an understanding of processes that control and influence the ecology of the Cape Fear River, and to provide a mechanism for information exchange and public education. This program provides a forum for dialogue among the various Cape Fear River user groups and encourages interaction among them. Overall policy is set by an Advisory Board consisting of representatives from citizen’s groups, local government, industries, academia, the business community, and regulatory agencies. This report represents the scientific conclusions of the UNCW researchers participating in this program and does not necessarily reflect opinions of all other program participants. This report focuses on the period January through December 2018. The scientific basis of the LCFRP consists of the implementation of an ongoing comprehensive physical, chemical, and biological monitoring program. Another part of the mission is to develop and maintain a data base on the Cape Fear basin and make use of this data to develop management plans. Presently the program has amassed a 24-year (1995-2018) data base that is available to the public, and is used as a teaching tool. Using this monitoring data as a framework the program goals also include focused scientific projects and investigation of pollution episodes. The scientific aspects of the program are carried out by investigators from the University of North CarolinaWilmington Center for Marine Science. The monitoring program was developed by the Lower Cape Fear River Program Technical Committee, which consists of representatives from UNCW, the North Carolina Division of Environmental Quality, The NC Division of Marine Fisheries, the US Army Corps of Engineers, technical representatives from streamside industries, the Cape Fear Public Utility Authority, CapeFear Community College, Cape Fear River Watch, the North Carolina Cooperative Extension Service, the US Geological Survey, forestry and agriculture organizations, and others. This integrated and cooperative program was the first of its kind in North Carolina. The physical, chemical and biological data are state-certified and submitted to the US EPA. Broad-scale monthly water quality sampling at 16 stations in the estuary and lower river system began in June 1995 (UNCW Aquatic Ecology Laboratory, directed by Dr. Michael Mallin). Sampling was increased to 34 stations in February of 1996, 35 stations in February 1998, and 36 stations in 2005, then lowered to 33 in 2011; currently it stands at 32 water quality stations. The Lower Cape Fear River Program added another component concerned with studying the benthic macrofauna of the system in 1996. This component is directed by Dr. Martin Posey and Mr. Troy Alphin of the UNCW Biology Department and includes the benefit of additional data collected by the 5 Benthic Ecology Laboratory under various grant-funded projects in the Cape Fear Estuary. These data are collected and analyzed depending upon the availability of funding. The third major biotic component (added in January 1996) was an extensive fisheries program directed by Dr. Mary Moser of the UNCW Center for Marine Science Research, with subsequent (1999) overseeing by Mr. Michael Williams and Dr. Thomas Lankford of UNCW-CMS. This program involved cooperative sampling with the North Carolina Division of Marine Fisheries and the North Carolina Wildlife Resources Commission. The fisheries program ended in December 1999, but was renewed with additional funds from the Z. Smith Reynolds Foundation from spring – winter 2000. 1.1. Site Description The mainstem of the Cape Fear River is formed by the merging of the Haw and the Deep Rivers in Chatham County in the North Carolina Piedmont. However, its drainage basin reaches as far upstream as the Greensboro area (Fig. 1.1). The mainstem of the river has been altered by the construction of several dams and water control structures. In the Coastal Plain, the river is joined by two major tributaries, the Black and the Northeast Cape Fear Rivers (Fig. 1.1). These 5th order blackwater streams drain extensive riverine swamp forests and add organic color to the mainstem. The watershed (about 9,164 square miles) is the most heavily industrialized in North Carolina with 203 permitted wastewater discharges with a permitted flow of approximately 429 million gallons per day, and (as of 2010) over 2.07 million people residing in the basin (NCDENR Basinwide Information Management System (BIMS) & 2010 Census). Approximately 23% of the land use in the watershed is devoted to agriculture and livestock production (2006 National Land Cover Dataset), with livestock production dominated by swine and poultry operations. Thus, the watershed receives considerable point and non-point source loading of pollutants. However, the estuary is a well-flushed system, with flushing time ranging from 1 to 22 days with a median flushing time of about seven days, much shorter than the other large N.C. estuaries to the north (Ensign et al. 2004). Water quality is monitored by boat at eight stations in the Cape Fear Estuary (from Navassa to Southport) and one station in the Northeast Cape Fear Estuary (Table 1.1; Fig. 1.1). We note that after July 2011 sampling was discontinued at estuarine stations M42 and SPD, per agreement with the North Carolina Division of Water Quality; and in 2012 sampling was expanded at Smith Creek at the Castle Hayne Road bridge (Table 1.1) and initiated at a new site along the South River (SR-WC). Riverine stations sampled by boat include NC11, AC, DP, IC, and BBT (Table 1.1; Fig. 1.1). NC11 is located upstream of any major point source discharges in the lower river and estuary system, and is considered to be representative of water quality entering the lower system (we note that the City of Wilmington and portions of Brunswick County get their drinking water from the river just upstream of Lock and Dam #1). Station BBT is located on the Black River between Thoroughfare (a stream connecting the Cape Fear and Black Rivers) and the mainstem Cape Fear, and is influenced by both rivers. We consider B210 and NCF117 to represent water quality entering the lower Black and Northeast Cape Fear Rivers, respectively. Data has also been collected at stream and 6 river stations throughout the Cape Fear, Northeast Cape Fear, and Black River watersheds (Table 1.1; Fig. 1.1; Mallin et al. 2001). 1.2. Report Organization Section 1 of this report provides a summary and introduction, and Section 2 of this report presents a detailed overview of physical, chemical, and biological water quality data from the 32 individual stations, and provides tables of raw data as well as figures showing spatial or temporal trends. LCFRP data are freely available to the public. The LCFRP has a website that contains maps and an extensive amount of past water quality, benthos, and fisheries data gathered by the Program available at: www.uncw.edu/cms/aelab/LCFRP/. Additionally, there is an on-line data base. http://lcfrp.uncw.edu/riverdatabase/ References Cited Ensign, S.H., J.N. Halls and M.A. Mallin. 2004. Application of digital bathymetry data in an analysis of flushing times of two North Carolina estuaries. Computers and Geosciences 30:501-511. Mallin, M.A., S.H. Ensign, M.R. McIver, G.C. Shank and P.K. Fowler. 2001. Demographic, landscape, and meteorological factors controlling the microbial pollution of coastal waters. Hydrobiologia 460:185-193. 7 Table 1.1 Description of sampling locations for the Lower Cape Fear River Program, 2018. Collected by Boat AEL Station DWR Station #Description Comments County LatLon Stream Class.HUC NC11 B8360000 Cape Fear River at NC 11 nr East Arcadia Below Lock and Dam 1, Represents water entering lower basin Bladen 34.3969-78.2675 WS-IV Sw 03030005 AC B8450000 Cape Fear River at Neils Eddy Landing nr Acme 1 mile below IP, DWR ambient station Columbus 34.3555-78.1794 C Sw 03030005 DP B8465000 Cape Fear River at Intake nr Hooper Hill AT DAK intake, just above confluence with Black R.Brunswick 34.3358-78.0534 C Sw 03030005 BBT Black River below Lyons Thorofare UNCW AEL station Pender 34.3513-78.0490 C Sw ORW+0303005 IC B9030000 Cape Fear River ups Indian Creek nr Phoenix Downstream of several point source discharges Brunswick 34.3021-78.0137 C Sw 0303005 NAV B9050025 Cape Fear River dns of RR bridge at Navassa Downstream of several point source discharges Brunswick 34.2594-77.9877 SC 0303005 HB B9050100 Cape Fear River at S. end of Horseshoe Bend nr Wilmington Upstream of confluence with NE Cape Fear River Brunswick 34.2437-77.9698 SC 0303005 BRR B9790000 Brunswick River dns NC 17 at park nr Belville Near Belville discharge Brunswick 34.2214-77.9787 SC 03030005 M61B9800000 Cape Fear River at Channel Marker 61 at Wilmington Downstream of several point source discharges New Hanover 34.1938-77.9573 SC 03030005 M54B9795000 Cape Fear River at Channel Marker 54 Downstream of several point source discharges New Hanover 34.1393-77.946 SC 03030005 M35B9850100 Cape Fear River at Channel Marker 35 Upstream of Carolina Beach discharge Brunswick 34.0335-77.937 SC 03030005 M23B9910000 Cape Fear River at Channel Marker 23 Downstream of Carolina Beach discharge Brunswick 33.9456-77.9696 SA HQW 03030005 M18B9921000 Cape Fear River at Channel Marker 18 Near mouth of Cape Fear River Brunswick 33.913-78.017 SC 03030005 NCF6 B9670000 NE Cape Fear nr Wrightsboro Downstream of several point source discharges New Hanover 34.3171-77.9538 C Sw 0303007 Collected by Land 6RC B8740000 Six Runs Creek at SR 1003 nr Ingold Upstream of Black River, CAFOs in watershed Sampson 34.7933-78.3113 C Sw ORW+03030006 LCO B8610001 Little Coharie Creek at SR 1207 nr Ingold Upstream of Great Coharie, CAFOs in watershed Sampson 34.8347-78.3709 C Sw 03030006 GCO B8604000 Great Coharie Creek at SR 1214 nr Butler Crossroads Downstream of Clinton, CAFOs in watershed Sampson 34.9186-78.3887 C Sw 03030006 SR B8470000 South River at US 13 nr CooperDownstream of Dunn Sampson 35.156-78.6401 C Sw 03030006 BRN B8340050 Browns Creek at NC87 nr Elizabethtown CAFOs in watershed Bladen 34.6136-78.5848 C 03030005 HAM B8340200 Hammond Creek at SR 1704 nr Mt. Olive CAFOs in watershed Bladen 34.5685-78.5515 C 03030005 COL B8981000 Colly Creek at NC 53 at Colly Pristine area Bladen 34.4641-78.2569 C Sw 03030006 B210B9000000 Black River at NC 210 at Still Bluff 1st bridge upstream of Cape Fear River Pender 34.4312-78.1441 C Sw ORW+03030006 NC403B9090000 NE Cape Fear River at NC 403 nr Williams Downstream of Mt. Olive Pickle, CAFOs in watershed Duplin 35.1784-77.9807 C Sw 0303007 PB B9130000 Panther Branch (Creek) nr Faison Downstream of Bay Valley Foods Duplin 35.1345-78.1363 C Sw 0303007 GS B9191000 Goshen Swamp at NC 11 and NC 903 nr Kornegay CAFOs in watershed Duplin 35.0281-77.8516 C Sw 0303007 SAR B9191500 NE Cape Fear River SR 1700 nr Sarecta Downstream of several point source discharges Duplin 34.9801-77.8622 C Sw 0303007 ROC B9430000 Rockfish Creek at US 117 nr WallaceUpstream of Wallace discharge Duplin 34.7168-77.9795 C Sw 0303007 LRC B9460000 Little Rockfish Creek at NC 11 nr Wallace DWR Benthic station Duplin 34.7224-77.9814 C Sw 0303007 ANC B9490000 Angola Creek at NC 53 nr Maple HillDWR Benthic station Pender 34.6562-77.7351 C Sw 0303007 SR WC B8920000 South River at SR 1007 (Wildcat/Ennis Bridge Road)Upstream of Black River Sampson 34.6402-78.3116 C Sw ORW+03030006 NCF117B9580000 NE Cape Fear River at US 117 at Castle Hayne DWR ambient station, Downstream of point source discharges New Hanover 34.3637-77.8965 B Sw 0303007 SC-CH B9720000 Smith Creek at US 117 and NC 133 at Wilmington Urban runoff, Downstream of Wilmington Northside WWTP New Hanover 34.2586-77.9391 C Sw 0303007 8 Figure 1.1. Map of the Lower Cape Fear River system and the LCFRP sampling stations. 9 2.0 Physical, Chemical, and Biological Characteristics of the Lower Cape Fear River and Estuary Michael A. Mallin and Matthew R. McIver Aquatic Ecology Laboratory Center for Marine Science University of North Carolina Wilmington 2.1 - Introduction This section of the report includes a discussion of the physical, chemical, and biological water quality parameters, concentrating on the January-December 2018 Lower Cape Fear River Program monitoring period. These parameters are interdependent and define the overall condition of the river. Physical parameters measured during this study included water temperature, dissolved oxygen, field turbidity and laboratory turbidity, total suspended solids (TSS), salinity, conductivity, pH and light attenuation. The chemical makeup of the Cape Fear River was investigated by measuring the magnitude and composition of nitrogen and phosphorus in the water, and metals as requested by NCDEQ. Selected biological parameters including fecal coliform bacteria or Enterococcus bacteria and chlorophyll a were examined. 2.2 - Materials and Methods Samples and field parameters collected for the estuarine stations of the Cape Fear River (NAV down through M18) were gathered (when possible) on an ebb tide. This was done so that the data better represented the river water flowing downstream through the system rather than the tidal influx of coastal ocean water. Sample collection and analyses were conducted according to the procedures in the Lower Cape Fear River Program Quality Assurance/Quality Control (QA/QC) manual. Technical Representatives from the LCFRP Technical Committee and representatives from the NC Division of Environmental Quality inspect UNCW laboratory procedures and periodically accompany field teams to verify proper procedures are followed. By agreement with N.C. Division of Environmental Quality, changes have periodically occurred in the sampling regime. Station SCCH (lower Smith Creek) was added October 2004; sampling was discontinued at Stations M42 and SPD (June 2011); sampling at Stations BCRR and BC117 was discontinued (December 2012); sampling was added at Station SR-WC on the South River (March 2013); and sampling was discontinued at Station LVC2 (July 2015). Special sampling for dissolved metals was initiated at selected stations by NCDEQ in 2015 and is ongoing. Physical Parameters Water Temperature, pH, Dissolved Oxygen, Turbidity, Light, Salinity, Conductivity Field parameters other than light attenuation were measured at each site using a YSI 6920/650 MDS or YSI EXO3. Each parameter is measured with individual probes on the sonde. At stations sampled by boat (see Table 1.1) physical parameters were measured 10 at 0.1 m and at the bottom (up to 12 m); only surface data are reported within. Occasionally, high flow prohibited the sonde from reaching the actual bottom and measurements were taken as deep as possible. At the terrestrially sampled stations (i.e. from bridges or docks) the physical parameters were measured at a depth of 0.1 m. The Aquatic Ecology Laboratory at the UNCW CMS is State-certified by the N.C. Division of Environmental Quality to perform field parameter measurements. The light attenuation coefficient k was determined from data collected on-site using vertical profiles obtained by a Li-Cor LI-1000 integrator interfaced with a Li-Cor LI-193S spherical quantum sensor. Chemical Parameters Nutrients A local State-certified analytical laboratory was contracted to conduct all chemical analyses except for orthophosphate, which is performed at CMS. The following methods detail the techniques used by CMS personnel for orthophosphate analysis. Orthophosphate (PO4-3) Water samples were collected ca. 0.1 m below the surface in triplicate in amber 125 mL Nalgene plastic bottles and placed on ice. In the laboratory 50 mL of each triplicate was filtered through separate1.0 micron pre-combusted glass fiber filters, which were frozen and later analyzed for chlorophyll a. The triplicate filtrates were pooled in a glass flask, mixed thoroughly, and approximately 100 mL was poured into a 125 mL plastic bottle to be analyzed for orthophosphate. Samples were frozen until analysis. Orthophosphate analyses were performed in duplicate using an approved US EPA method for the Bran-Lubbe AutoAnalyzer (Method 365.5). In this technique the orthophosphate in each sample reacts with ammonium molybdate and anitmony potassium tartrate in an acidic medium (sulfuric acid) to form an anitmony-phospho-molybdate complex. The complex is then reacted with ascorbic acid and forms a deep blue color. The intensity of the color is measured at a wavelength of 880 nm by a colorimeter and displayed on a chart recorder. Standards and spiked samples were analyzed for quality assurance. Biological Parameters Fecal Coliform Bacteria / Enterococcus Fecal coliform bacteria were analyzed by a State-certified laboratory contracted by the LCFRP. Samples were collected approximately 0.1 m below the surface in sterile plastic bottles provided by the contract laboratory and placed on ice for no more than six hours before analysis. After August 2011 the fecal coliform analysis was changed to Enterococcus bacteria in the estuarine stations downstream of NAV and HB (Stations BRR, M61, M35, M23 and M18). 11 Chlorophyll a The analytical method used to measure chlorophyll a is described in Welschmeyer (1994) and US EPA (1997) and was performed by UNCW Aquatic Ecology Laboratory personnel. Chlorophyll a concentrations were determined utilizing the 1.0 micron filters used for filtering samples for orthophosphate analysis. All filters were wrapped individually in foil, placed in airtight containers and stored in the freezer. During analysis each filter was immersed in 10 mL of 90% acetone for 24 hours, which extracts the chlorophyll a into solution. Chlorophyll a concentration of each solution was measured on a Turner 10-AU fluorometer. The fluorometer uses an optimal combination of excitation and emission bandwidth filters which reduces the errors inherent in the acidification technique. The Aquatic Ecology Laboratory at the CMS is State-certified by the N.C. Division of Environmental Quality for the analysis of chlorophyll a (chlorophyll at three LCFRP stations are required by NCDEQ to be analyzed by state-certified methods); the rest of the large amount of chlorophyll a data presented here were not State-certified. Biochemical Oxygen Demand (BOD) Five sites were originally chosen for BOD analysis. One site was located at NC11, upstream of International Paper, and a second site was at AC, about 3 miles downstream of International Paper (Fig.1.1). Two sites were located in blackwater rivers (NCF117 and B210) and one site (BBT) was situated in an area influenced by both the mainstem Cape Fear River and the Black River. For the sampling period May 2000-April 2004 additional BOD data were collected at stream stations 6RC, LCO, GCO, BRN, HAM and COL in the Cape Fear and Black River watersheds. In May 2004 those stations were dropped and sampling commenced at ANC, SAR, GS, N403, ROC and BC117 in the Northeast Cape Fear River watershed for several years. BOD analysis was stopped in August 2015 due to insufficient program funding; previous BOD data are available from LCFRP. Parameter Method NC DEQ Certified Water Temperature SM 2550B-2000 Yes Dissolved Oxygen SM 4500O G-2001 Yes pH SM 4500 H B-2000 Yes Specific Conductivity SM 2510 B-1997 Yes Lab Turbidity SM 2130 B-2001 Yes Field Turbidity SM 2130 B-2001 No Chlorophyll a EPA 445.0 Rev. 1.2 Yes Biochemical Oxygen Demand SM 5210 B-2001 No 12 Parameter Method NC DEQ Certified Total Nitrogen By addition Nitrate + Nitrite EPA 353.2 Rev 2.0 1993 Yes Total Kjeldahl Nitrogen EPA 351.2 Rev 2.0 1993 Yes Ammonia Nitrogen EPA 350.1 Rev 2.0 1993 Yes Total Phosphorus SM 4500 P E-1999 Yes Orthophosphate EPA 365.5 No Fecal Coliform SM 9222 D-1997 Yes Enterococcus Enterolert IDEXX Yes 2.3 - Results and Discussion This section includes results from monitoring of the physical, biological, and chemical parameters at all stations for the time period January-December 2018. Discussion of the data focuses both on the river channel stations and stream stations, which sometimes reflect poorer water quality than the channel stations. The contributions of the two large blackwater tributaries, the Northeast Cape Fear River and the Black River, are represented by conditions at NCF117 and B210, respectively. As noted, the Cape Fear region experienced major impacts from Hurricane Florence in 2018; therefore this report reflects the impacts in fall; note that September sampling was missing from some stations due to lack of access. Physical Parameters Water temperature Water temperatures at all stations ranged from 3.1 to 31.3oC, and individual station annual averages ranged from 15.2 to 19.1oC (Table 2.1). Highest temperatures occurred during July and lowest temperatures during January and February. Stream stations were generally cooler than river stations, most likely because of shading and lower nighttime air temperatures affecting the shallower waters. Salinity Salinity at the estuarine stations (NAV through M18; also NCF6 in the Northeast Cape Fear River) ranged from 0.0 to 31.4 practical salinity units (psu) and station annual means ranged from 0.1 to 22.3 psu (Table 2.2). Lowest salinities occurred in late spring and again in October following the heavy rains from the hurricane. As such, the annual mean salinities for 2018 were lower compared with the twenty-one year average for 1995-2017 (Figure 2.1). Two stream stations, NC403 and PB, had occasional oligohaline conditions due to discharges from pickle production facilities. SC-CH is a blackwater tidal creek that 13 enters the Northeast Cape Fear River upstream of Wilmington and salinity there ranged from 0.1 to 2.4 psu. Conductivity Conductivity at the estuarine stations ranged from 0.09 to 48.16 mS/cm and from 0.05 to 4.56 mS/cm at the freshwater stations (Table 2.3). Temporal conductivity patterns followed those of salinity. Dissolved ionic compounds increase the conductance of water, therefore, conductance increases and decreases with salinity, often reflecting river flow conditions due to rainfall. Stations PB and NC403 are below industrial discharges, and often have elevated conductivity. Smith Creek (SC-CH) is an estuarine tidal creek and the conductivity values reflect this (Table 2.3). pH System pH values ranged from 3.5 to 8.1 and station annual means ranged from 4.1 to 8.0 (Table 2.4). pH was typically lowest upstream due to acidic swamp water inputs and highest downstream as alkaline seawater mixes with the river water. Low pH values at COL predominate because of naturally acidic blackwater inputs in this wetland-rich rural watershed. Dissolved Oxygen Dissolved oxygen (DO) problems have long been a major water quality concern in the lower Cape Fear River and its estuary, and several of the tributary streams (Mallin et al. 1999; 2000; 2001; 2002; 2004; 2005; 2006). There is an annual dissolved oxygen sag in the main river channel that begins at DP below a paper mill discharge and persists into the mesohaline portion of the estuary (Fig. 2.2). Working synergistically to lower oxygen levels are two factors: lower oxygen carrying capacity in warmer water and increased bacterial respiration (or biochemical oxygen demand, BOD), due to higher temperatures in summer. Unlike other large North Carolina estuaries (the Neuse, Pamlico and New River) the Cape Fear estuary rarely suffers from dissolved oxygen stratification. This is because, despite salinity stratification the oxygen remains well mixed due to strong estuarine gravitational circulation and high freshwater inputs (Lin et al. 2006). Thus, hypoxia in the Cape Fear is present throughout the water column. Surface concentrations for all sites in 2018 ranged from 0.2 to 13.6 mg/L and station annual means ranged from 5.8 to 8.3 mg/L (Table 2.5). Overall, average dissolved oxygen levels were mixed in 2018 compared with the long-term average (Fig. 2.2). River dissolved oxygen levels were low during the summer (Table 2.5), often falling below the state standard of 5.0 mg/L at several river and upper estuary stations. While DO concentrations were already low in summer the September arrival of Hurricane Florence brought approximately two feet of rain to the area, caused a massive BOD load from animal waste, sewage and natural swamp organic matter to drop DO lower than usual in October. Hurricane Florence led to the release of many millions of gallons of untreated or partially treated sewage due to system flooding, power failures, generator 14 damage and pump station failures. According to reported data, Fayetteville had > 7.6 million gallons reaching surface waters, Wilmington had to reroute 5.25 million gallons into the river, and Carolina Beach had to discharge 17.2 million gallons to the estuary. According to industry self-reporting, 49 swine waste lagoons serving concentrated animal feeding operations (CAFOs) either breached, overtopped or were inundated by floodwaters, and another 47 lagoons were possibly compromised by high freeboard in the lagoons. Many thousands of acres of swine sprayfields and poultry CAFO litterfields were inundated by floodwaters as well. The Northeast Cape Fear River stations (NCF117 and BCF6) were particularly hard hit by the BOD load, with DO dropping to 0.2 mg/L for several weeks. This caused massive fish kills in the system (see front cover) including endangered sturgeon Overall for the year, stations M61 and M54 were below 5.0 mg/L on 45% or more of occasions sampled, NAV was below on 36% of occasions sampled, and HB and M35 below standard 27% of th etime. Based on number of occasions the river stations were below 5 mg/L UNCW rated NAV, HB, M61, M54, M35 and BRR as poor for 2018; the lower estuary stations were rated as good. On a year-to-year basis, discharge of BOD waste from the paper/pulp mill just above the AC station (Mallin et al. 2003), as well as inflow of blackwater from the Northeast Cape Fear and Black Rivers, helps to decrease oxygen in the lower river and upper estuary. Additionally, algal blooms periodically form behind Lock and Dam #1 (including the blue-green algal blooms from 2009-2012), and the chlorophyll a they produce is strongly correlated with BOD at Station NC11 (Mallin et al. 2006); thus algal blooms do contribute to lower DO in the river. As the water reaches the lower estuary higher algal productivity, mixing and ocean dilution help alleviate oxygen problems. We note that due to the hurricane, DO conditions in the lower river and estuary in 2018 were worse than 2017. The hurricane impacted the tributary stream stations in October, but DO levels recovered in November. Tributary Stations SC-CH and GS were below 4.0 mg/L on 27% of occasions sampled (rated poor), and NC403 and ANC 18% (rated fair) most others were in the good category (Table 2.5). Some hypoxia can be attributed to low summer water conditions and some potentially to CAFO runoff; however point-source discharges also likely contribute to low dissolved oxygen levels at NC403 and possibly SR, especially via nutrient loading (Mallin et al. 2001; 2002; 2004). Hypoxia is thus a continuing problem, with 32% of the sites impacted in 2017. Field Turbidity Field turbidity levels ranged from 0 to 87 Nephelometric turbidity units (NTU) and station annual means ranged from 3 to 26 NTU (Table 2.6). The State standard for estuarine turbidity is 25 NTU. Highest mean turbidities were at NC11-DP (26-19 NTU), with turbidities generally low in the middle to lower estuary (Figure 2.3). The estuarine stations did not exceed the estuarine turbidity standard on our 2018 sampling trips. As in the previous year, mean turbidity levels for 2018 were well below the long-term average at all estuary sites (Fig. 2.3). Turbidity was considerably lower in the blackwater tributaries (Northeast Cape Fear River and Black River) than in the mainstem river. Average turbidity 15 levels were low in the freshwater streams. The State standard for freshwater turbidity is 50 NTU. Note: In addition to the laboratory-analyzed turbidity that are required by NCDWQ for seven locations, the LCFRP uses nephelometers designed for field use, which allows us to acquire in situ turbidity from a natural situation. North Carolina regulatory agencies are required to use turbidity values from water samples removed from the natural system, put on ice until arrival at a State-certified laboratory, and analyzed using laboratory nephelometers. Standard Methods notes that transport of samples and temperature change alters true turbidity readings. Our analysis of samples using both methods shows that lab turbidity is nearly always lower than field turbidity; thus we do not discuss lab turbidity in this report. Total Suspended Solids (TSS) An altered monitoring plan was developed for the LCFRP in September 2011. These changes were suggested by the NC Division of Environmental Quality (then DWQ). NCDEQ suggested the LCFRP stop monitoring TSS at Stations ANC, GS, 6RC, LCO, SR, BRN, HAM, COL, SR-WC and monitor turbidity instead. DWQ believed turbidity would be more useful than TSS in evaluating water quality at these stations because there are water quality standards for turbidity. TSS is used by the DWQ NPDES Unit to evaluate discharges. No LCFRP subscribers discharge in these areas. Total suspended solid (TSS) values system wide ranged from 1.3 to 85.0 mg/L with station annual means from 2.3 to 27.3 mg/L (Table 2.7). The overall highest river values were at NC11 and AC. In the stream stations TSS was generally considerably lower than the river and estuary. Although total suspended solids (TSS) and turbidity both quantify suspended material in the water column, they do not always go hand in hand. High TSS does not mean high turbidity and vice versa. This anomaly may be explained by the fact that fine clay particles are effective at dispersing light and causing high turbidity readings, while not resulting in high TSS. On the other hand, large organic or inorganic particles may be less effective at dispersing light, yet their greater mass results in high TSS levels. While there is no NC ambient standard for TSS, many years of data from the lower Cape Fear watershed indicates that 25 mg/L can be considered elevated (reached on a few occasions in the 2018 data). The fine silt and clay in the upper to middle estuary sediments are most likely derived from the Piedmont and carried downstream to the estuary, while the sediments in the lowest portion of the estuary are marine-derived sands (Benedetti et al. 2006). Light Attenuation The attenuation of solar irradiance through the water column is measured by a logarithmic function (k) per meter. The higher this light attenuation coefficient is the more strongly light is attenuated (reduced through absorbance or reflection) in the water column. River and estuary light attenuation coefficients ranged from 0.74 to 7.10/m and station annual means ranged from 2.23 at M18 to 3.98 at NAV (Table 2.8). Elevated mean and median light 16 attenuation occurred from NC11 river downstream to M54 in the estuary (Table 2.8). In the Cape Fear system, light is attenuated by both turbidity and water color. High light attenuation did not always coincide with high turbidity. Blackwater, though low in turbidity, will attenuate light through absorption of solar irradiance. At NCF6 and BBT, blackwater stations with moderate turbidity levels, light attenuation was high. Compared to other North Carolina estuaries the Cape Fear has generally high light attenuation. The high average light attenuation is a major reason why phytoplankton production in the major rivers and the estuary of the LCFR is generally low. Whether caused by turbidity or water color this attenuation tends to limit light availability to the phytoplankton (Mallin et al. 1997; 1999; 2004; Dubbs and Whalen 2008). Chemical Parameters – Nutrients Total Nitrogen Total nitrogen (TN) is calculated from TKN (see below) plus nitrate; it is not analyzed in the laboratory. TN ranged from 50 (detection limit) to 6,480 µg/L (at ROC) and station annual means ranged from 657 to 2,804 µg/L (at NC403; Table 2.9). Previous research (Mallin et al. 1999) has shown a positive correlation between river flow and TN in the Cape Fear system. In the main river total nitrogen concentrations were highest and relatively similar between NC11 and AC, then declining into the lower estuary, most likely reflecting uptake of nitrogen into the food chain through algal productivity and subsequent grazing by planktivores as well as through dilution and marsh denitrification. The highest median TN value at the stream stations was at NC403 with 2,540 µg/L; other elevated TN values were seen at ANC, ROC, 6RC and PB. Nitrate+Nitrite Nitrate+nitrite (henceforth referred to as nitrate) is the main species of inorganic nitrogen in the Lower Cape Fear River. Concentrations system wide ranged from 10 (detection limit) to 4,430 µg/L (at PB) and station annual means ranged from 24 to 1,858 µg/L (at NC403; Table 2.10). The highest average riverine nitrate levels were at NC11, AC and DP (734- 660 µg/L) indicating that much of this nutrient is imported from upstream. Moving downstream, nitrate levels decrease most likely as a result of uptake by primary producers, microbial denitrification in riparian marshes and tidal dilution. Despite this, the rapid flushing of the estuary (Ensign et al. 2004) permits sufficient nitrate to enter the coastal ocean in the plume and contribute to offshore productivity (Mallin et al. 2005). Nitrate can limit phytoplankton production in the lower estuary in summer (Mallin et al. 1999). The blackwater rivers carried lower concentrations of nitrate compared to the mainstem Cape Fear stations; i.e. the Northeast Cape Fear River (NCF117 mean = 302 µg/L) and the Black River (B210 = 327 µg/L). Lowest river nitrate occurred during late spring and early summer. In general, average concentrations in 2018 for the mainstem river were lower than those of the average from 1995-2017 (Fig. 2.4); although nitrate concentrations post- hurricane (October and November were elevated in the rivers compared with pre-storm values (Table 2.10). 17 Several stream stations showed high levels of nitrate on occasion including NC403, PB, ROC and 6RC. ROC and 6RC primarily receive non-point agricultural or animal waste drainage, while point sources contribute to NC403 and PB. In general, the stream stations showed elevated nitrate in late fall and early winter following the massive runoff from the storm in September. A considerable number of experiments have been carried out by UNCW researchers to assess the effects of nutrient additions to water collected from blackwater streams and rivers (i.e. the Black and Northeast Cape Fear Rivers, and Colly and Great Coharie Creeks). These experiments have collectively found that additions of nitrogen (as either nitrate, ammonium, or urea) significantly stimulate phytoplankton production and BOD increases. Critical levels of these nutrients were in the range of 200 to 500 µg/L as N (Mallin et al. 1998; Mallin et al. 2001; Mallin et al. 2002, Mallin et al. 2004). Thus, we conservatively consider nitrate concentrations exceeding 500 µg/L as N in Cape Fear watershed streams to be potentially problematic to the stream’s environmental health. Ammonium/ammonia Ammonium concentrations ranged from 10 (detection limit) to 1,240 µg/L and station annual means ranged from 53 to 237 µg/L (Table 2.11). River areas with the highest mean ammonium levels this monitoring period included AC and DP, which are downstream of a pulp mill discharge, and M54 in the upper estuary near the Wilmington Southside Wastewater Treatment Plant. At the stream stations 2018 continued to be unusual in that Colly Creek (COL) showed multiple occasions of high ammonium. This station is in a wetland-rich watershed that has a low level of human development. Most previous years have showed generally low levels of ammonium; however, beginning in 2005 a few unusual peaks began to occur, which increased in magnitude and frequency after 2012, particularly in 2016, 2017 and 2018 (Fig. 2.6). We do not have a solid explanation for this increase in ammonium. We are aware that White Lake, located in the upper Colly Creek watershed has had increasing problems with eutrophication (NC DEQ 2017), with nearby upper groundwater and surface runoff showing elevated nutrient concentrations (especially ammonium; potentially from failing local sewage infrastructure in the densely-developed area immediately surrounding the lake). General nutrient concentrations in the lake have been increasing over time as well (NCDEQ 2017). Thus, possibly ammonium-rich drainage from this area has made its way down to the COL station. Additional areas with periodic elevated ammonium in 2018 included ANC, LRC and PB (Table 2.11). Total Kjeldahl Nitrogen Total Kjeldahl Nitrogen (TKN) is a measure of the total concentration of organic nitrogen plus ammonium. TKN ranged from 50 (detection limit) to 4,200 µg/L and station annual means ranged from 468 to 1,464 µg/L (Table 2.12). TKN concentration decreases ocean- ward through the estuary, likely due to ocean dilution and food chain uptake of nitrogen. Stations with highest median concentrations included ANC and COL. As with ammonium, 18 upper groundwater in the White Lake drainage contained high TKN (NC DEQ 2017), some of which may have gone downstream. Total Phosphorus Total phosphorus (TP) concentrations ranged from 10 (detection limit) to 1,390 µg/L (at ROC) and station annual means ranged from 45 to 305 µg/L (ROC; Table 2.13). For the mainstem and upper estuary, average TP for 2018 was higher than the 1995-2017 average; at all sites downstream of AC (Figure 2.6). In the river TP was highest at the upper riverine channel stations NC11, AC and DP and declined downstream into the estuary; there was an increase at M61, however. Some of this decline is attributable to the settling of phosphorus-bearing suspended sediments, yet incorporation of phosphorus into bacteria and algae is also responsible. The experiments discussed above in the nitrate subsection also involved additions of phosphorus, either as inorganic orthophosphate or a combination of inorganic plus organic P. The experiments showed that additions of P exceeding 500 µg/L led to significant increases in bacterial counts, as well as significant increases in BOD over control. Thus, we consider concentrations of phosphorus above 500 µg/L to be potentially problematic to blackwater streams (Mallin et al. 1998; 2004). Streams periodically exceeding this critical concentration included ROC, GCO and ANC; NC403 and PB also yielded some high values. Stations NC403 and PB are downstream of an industrial wastewater discharge, while ROC, GCO and ANC are in non-point agricultural areas. Orthophosphate Orthophosphate ranged from 5 to 480 µg/L and station annual means ranged from 6 to 170 µg/L (Table 2.14). Much of the main river orthophosphate load is imported into the Lower Cape Fear system from upstream areas, as NC11 or AC typically have high levels; there are also inputs of orthophosphate from the paper mill above AC (Table 2.140. Orthophosphate can bind to suspended materials and is transported downstream via particle attachment; thus high levels of turbidity at the uppermost river stations may be an important factor in the high orthophosphate levels. Turbidity declines toward the lower estuary because of settling, and orthophosphate concentration also declines. In the estuary, primary productivity helps reduce orthophosphate concentrations by assimilation into biomass. Orthophosphate levels typically reach maximum concentrations during summertime, when anoxic sediment releases bound phosphorus. Also, in the Cape Fear Estuary, summer algal productivity is limited by nitrogen, thereby allowing the accumulation of orthophosphate (Mallin et al. 1997; 1999). In spring, productivity in the estuary is usually limited by phosphorus (Mallin et al. 1997; 1999). ROC, ANC and GCO had the highest stream station orthophosphate concentrations. All of those sites are in non-point source areas. 19 Chemical Parameters - EPA Priority Pollutant Metals The LCFRP had previously sampled for water column metals (EPA Priority Pollutant Metals) on a bimonthly basis. However, as of 2007 this requirement was suspended by the NC Division of Water Quality and these data are no longer collected by the LCFRP. Revised metals sampling (dissolved, not total metals) was re-initiated in late 2015 and continued periodically upon request from NCDEQ. Results showed that for both stations sampled (M35 and M23) concentrations of As, Cd, Cr, Cu, Pb, Ni and Zn were below detection limits on all sampling occasions. Iron (Fe) concentrations were measurable but not at harmful levels. There was one metals sample collected in December 2018 at IC and NAV, with no unusual or adversely high concentrations. Two more samples are scheduled for those locations for 2019. Biological Parameters Chlorophyll a During this monitoring period, chlorophyll a was low in river and estuary locations (Table 2.15). The state standard was not exceeded in the river or estuary samples in 2018. We note that at the upper site NC11 it has been demonstrated that chlorophyll a biomass is significantly correlated with biochemical oxygen demand (BOD5 – Mallin et al. 2006). System wide, chlorophyll a ranged from undetectable to 43 µg/L, and station annual means ranged from 1-13 µg/L, generally low because of high river discharge in 2018 (see below). Production of chlorophyll a biomass is usually low to moderate in the rivers and estuary primarily because of light limitation by turbidity in the mainstem (Dubbs and Whalen 2008) and high organic color and low inorganic nutrients in the blackwater tributary rivers. Spatially, along the river mainstem highest values are normally found in the mid-to-lower estuary stations because light becomes more available downstream of the estuarine turbidity maximum (Fig. 2.7). On average, flushing time of the Cape Fear estuary is rapid, ranging from 1-22 days with a median of 6.7 days (Ensign et al. 2004). This does not allow for much settling of suspended materials, leading to light limitation of phytoplankton production. However, under lower-than-average flows there is generally clearer water through less suspended material and less blackwater swamp inputs. We note that there were a series of problematic cyanobacterial (blue-green algae) blooms of Microcyctis aeruginosa on the mainstem river in summers of 2009-2012 (Isaacs et al. 2014). For the growing season May-September, long-term (1995-2018) average monthly flow at Lock and Dam #1 was approximately 3,631 CFS; however, for cyanobacterial bloom years 2009- 2012 the growing season average flow was 1,698 CFS (USGS data; (http://nc.water.usgs.gov/realtime/real_time_cape_fear.html). For 2018, discharge in May-September (due to the hurricane) was more than triple the 2009-2012 average at 8,590 CFS. Nuisance cyanobacterial blooms did not occur in the river and upper estuary in 2018. 20 The blooms in 2009-2012 all occurred when average river discharge for May-September was below 1,900 CFS. Algal bloom formation was suppressed by elevated river flow in 2013-2014, 2016 and 2018, but flow in 2015 was well within the range when blooms can occur. Clearly other factors are at work in bloom formation. Phytoplankton blooms occasionally occur at the stream stations, with a few occurring at various months in 2018 (Table 2.15). These streams are generally shallow, so vertical mixing does not carry phytoplankton cells down below the critical depth where respiration exceeds photosynthesis. In areas where the forest canopy opens up large blooms can occur. When blooms occur in blackwater streams they can become sources of BOD upon death and decay, reducing further the low summer dissolved oxygen conditions common to these waters (Mallin et al. 2001; 2002; 2004; 2006; 2015). A stream station bloom exceeding the state standard of 40 µg/L occurred on one occasion at Station SR, and lesser blooms occurred on occasion at PB, N403, ROC, ANC and GS (Table 2.15). Biochemical Oxygen Demand Beginning in 2015 samples for BOD5 and BOD20 are no longer collected for the program due to insufficient funds. Fecal Coliform Bacteria/ Enterococcus bacteria Fecal coliform (FC) bacterial counts ranged from 5 to 60,000 CFU/100 mL (60,000 is the laboratory maximum) and station annual geometric means ranged from 12 to 340 CFU/100 mL (Table 2.17). The state human contact standard (200 CFU/100 mL) was exceeded in the mainstem river on a few occasions in 2018 (Table 2.17). The Northeast Cape Fear River showed unusually high fecal coliform counts in 2018, with NCF6 and NCF117 both exceeding the standard on three occasions. During 2018 some stream stations showed very high fecal coliform pollution levels. HAM exceeded 200 CFU/100 mL 73% of the time sampled; ROC 64%, BRN 55%, PB and LCO 46%, NC403 and SCH 36% and ANC and LRC 27% of the time sampled. One notably excessive count of 60,000 CFU/100 mL occurred at PB in July, while most other counts stayed below 2,000 CFU/100 mL. NC403 and PB are located below point source discharges and the other sites are primarily influenced by non-point source pollution. Beginning in 2015 but especially in 2017 COL had a number of unusually high fecal coliform counts; but counts were much lesser in 2018 (Fig. 2.5). Overall, 2018 was comparatively better than previous years, despite the hurricane. In fact, at most chronically polluted sites high counts were in spring and summer months before the hurricane, whereas the high counts immediately after the hurricane decreased rapidly after October (Table 2.16; Fig. 2.8). We speculate that the extreme rainfall and river discharge (25,600 CFS at Lock and Dam #1 in September) diluted fecal bacteria counts, despite the CAFO accidents and sewage discharges. Enterococcus counts were initiated in the estuary in mid-2011, as this test is now the standard used by North Carolina regulators for swimming in salt waters. Sites covered by this test include BRR, M61, M54, M35, M23 and M18. The State has a single-sample level for Tier II swimming areas in which the enterococci level in a Tier II swimming area shall 21 not exceed a single sample of 276 enterococci per 100 milliliter of water (15A NCAC 18A .3402); the LCFRP is using this standard for the Cape Fear estuary samples in our rating system. As such, in 2018 this standard was not exceeded in the estuary samples. Geometric mean enterococcus counts for 2018 were lower than those of the 2012-2017 period for the lower Cape Fear Estuary (Fig. 2.8). Overall, elevated fecal coliform and Enterococcus counts are problematic in this system, with 61% of the stations rated as Fair or Poor in 2018 (although that was an improvement from 2017). 2.4 - References Cited APHA. 1995. Standard Methods for the Examination of Water and Wastewater, 19th ed. American Public Health Association, Washington, D.C. Benedetti, M.M., M.J. Raber, M.S. Smith and L.A. Leonard. 2006. Mineralogical indicators of alluvial sediment sources in the Cape Fear River basin, North Carolina. Physical Geography 27:258-281. Dubbs, L. L. and S.C. Whalen. 2008. Light-nutrient influences on biomass, photosynthetic potential and composition of suspended algal assemblages in the middle Cape Fear River, USA. International Review of Hydrobiology 93:711-730. Ensign, S.H., J.N. Halls and M.A. Mallin. 2004. Application of digital bathymetry data in an analysis of flushing times of two North Carolina estuaries. Computers and Geosciences 30:501-511. Isaacs, J.D., W.K. Strangman, M.A. Mallin, M.R. McIver, and J.L.C. Wright. 2014. Microcystins and two new micropeptin cyanopeptides produced by unprecedented Microcystis aeruginosa blooms in North Carolina’s Cape Fear River. Harmful Algae 31:82-86. Lin, J. L. Xie, L.J. Pietrafesa, J. Shen, M.A. Mallin and M.J. Durako. 2006. Dissolved oxygen stratification in two microtidal partially-mixed estuaries. Estuarine, Coastal and Shelf Science. 70:423-437. Mallin, M.A., L.B. Cahoon, M.R. McIver, D.C. Parsons and G.C. Shank. 1997. Nutrient limitation and eutrophication potential in the Cape Fear and New River Estuaries. Report No. 313. Water Resources Research Institute of the University of North Carolina, Raleigh, N.C. Mallin, M.A., L.B. Cahoon, D.C. Parsons and S.H. Ensign. 1998. Effect of organic and inorganic nutrient loading on photosynthetic and heterotrophic plankton communities in blackwater rivers. Report No. 315. Water Resources Research Institute of the University of North Carolina, Raleigh, N.C. Mallin, M.A., L.B. Cahoon, M.R. McIver, D.C. Parsons and G.C. Shank. 1999. Alternation of factors limiting phytoplankton production in the Cape Fear Estuary. Estuaries 22:985-996. 22 Mallin, M.A., M.H. Posey, M.R. McIver, S.H. Ensign, T.D. Alphin, M.S. Williams, M.L. Moser and J.F. Merritt. 2000. Environmental Assessment of the Lower Cape Fear River System, 1999-2000. CMS Report No. 00-01, Center for Marine Science, University of North Carolina at Wilmington, Wilmington, N.C. Mallin, M.A., L.B. Cahoon, D.C. Parsons and S.H. Ensign. 2001. Effect of nitrogen and phosphorus loading on plankton in Coastal Plain blackwater streams. Journal of Freshwater Ecology 16:455-466. Mallin, M.A., L.B. Cahoon, M.R. McIver and S.H. Ensign. 2002. Seeking science-based nutrient standards for coastal blackwater stream systems. Report No. 341. Water Resources Research Institute of the University of North Carolina, Raleigh, N.C. Mallin, M.A., M.R. McIver, S.H. Ensign and L.B. Cahoon. 2004. Photosynthetic and heterotrophic impacts of nutrient loading to blackwater streams. Ecological Applications 14:823-838. Mallin, M.A., L.B. Cahoon and M.J. Durako. 2005. Contrasting food-web support bases for adjoining river-influenced and non-river influenced continental shelf ecosystems. Estuarine, Coastal and Shelf Science 62:55-62. Mallin, M.A., V.L. Johnson, S.H. Ensign and T.A. MacPherson. 2006. Factors contributing to hypoxia in rivers, lakes and streams. Limnology and Oceanography 51:690-701. Mallin, M.A., M.R. McIver, A.R. Robuck and A.K. Dickens. 2015. Industrial swine and poultry production causes chronic nutrient and fecal microbial stream pollution. Water, Air and Soil Pollution 226:407, DOI 10.1007/s11270-015-2669-y. NCDEQ 2017. 2017 White Lake Water Quality Investigation, White Lake, Bladen County (Cape Fear Basin). North Carolina Department of Environmental Quality, Division of Water Resources. U.S. EPA 1997. Methods for the Determination of Chemical Substances in Marine and Estuarine Environmental Matrices, 2nd Ed. EPA/600/R-97/072. National Exposure Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, Ohio. Welschmeyer, N.A. 1994. Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and phaeopigments. Limnology and Oceanography 39:1985-1993. 23 Table 2.1 Water temperature (oC) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP BBT IC NCF6 JAN 3.0 3.7 4.0 4.0 3.9 4.1 4.2 4.7 JAN 4.4 5.1 4.4 2.4 3.2 4.3 FEB 7.9 8.5 8.5 8.8 9.4 9.9 9.3 9.4 FEB 8.4 8.1 8.0 8.0 8.2 8.5 MAR 12.4 13.0 12.1 12.8 13.0 13.6 13.1 12.8 MAR 11.8 11.9 11.8 13.1 12.3 13.3 APR 15.9 16.0 16.0 16.6 16.6 16.1 15.7 15.1 APR 15.8 16.5 16.3 16.8 16.2 16.7 MAY 25.4 25.7 25.7 25.3 25.4 25.4 25.8 25.3 MAY 17.9 17.9 18.1 19.8 20.3 20.1 JUN 27.7 26.7 29.2 27.8 28.6 28.0 27.7 27.2 JUN 27.2 27.0 27.3 26.2 27.1 26.0 JUL 30.5 31.3 31.0 31.0 30.7 30.8 30.1 29.9 JUL 28.8 28.9 29.3 28.9 28.7 27.5 AUG 27.7 27.8 28.1 27.6 27.9 28.2 28.7 28.6 AUG 27.1 27.3 27.2 27.3 27.4 26.3 SEP SEP 29.4 29.4 29.2 28.6 29.0 29.4 OCT 22.4 22.5 22.6 23.6 23.7 24.4 25.3 25.5 OCT 25.1 25.1 25.1 25.5 25.4 27.7 NOV 15.9 16.5 16.2 17.1 17.1 17.3 17.2 19.2 NOV 17.0 16.9 17.0 17.5 17.6 18.5 DEC 8.5 8.7 8.3 9.0 9.0 9.9 9.9 12.5 DEC 8.8 8.9 9.0 9.2 9.2 10.5 mean 17.9 18.2 18.3 18.5 18.7 18.9 18.8 19.1 mean 18.5 18.6 18.6 18.6 18.7 19.1 std dev 9.4 9.2 9.5 9.1 9.1 9.0 9.1 8.7 std dev 8.9 8.8 8.9 9.0 9.0 8.5 median 15.9 16.5 16.2 17.1 17.1 17.3 17.2 19.2 median 17.5 17.4 17.6 18.7 19.0 19.3 max 30.5 31.3 31.0 31.0 30.7 30.8 30.1 29.9 max 29.4 29.4 29.3 28.9 29.0 29.4 min 3.0 3.7 4.0 4.0 3.9 4.1 4.2 4.7 min 4.4 5.1 4.4 2.4 3.2 4.3 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SRWC 6RC LCO GCO SR BRN HAM JAN 5.7 3.1 4.5 3.6 3.2 5.2 4.2 8.1 7.5 JAN 6.8 5.5 7.4 7.3 6.5 5.0 5.8 6.2 6.6 FEB 11.2 12.0 11.6 11.2 10.3 11.7 12.1 9.1 11.1 FEB 12.8 13.4 12.9 14.6 14.3 12.7 11.8 12.8 12.0 MAR 9.2 6.2 8.6 7.3 7.0 9.1 8.3 11.2 11.5 MAR 10.8 10.8 10.8 11.3 11.4 11.0 13.3 16.7 15.2 APR 12.6 11.9 13.4 13.2 13.3 13.8 12.3 16.8 16.4 APR 18.1 17.2 16.1 16.4 16.5 17.0 17.4 17.8 17.1 MAY 18.6 18.4 21.0 20.2 18.0 18.6 17.3 18.7 19.9 MAY 20.0 18.3 19.9 19.0 19.9 20.3 20.5 20.0 19.4 JUN 23.7 24.1 25.2 24.2 24.6 24.8 23.1 25.0 25.5 JUN 24.7 23.7 24.0 24.1 24.2 25.0 25.6 24.5 24.1 JUL 26.0 26.8 26.1 26.8 27.3 26.6 25.8 28.5 28.1 JUL 27.0 24.8 25.3 25.2 25.4 26.4 27.7 25.2 24.5 AUG 26.0 25.8 26.0 26.2 26.8 24.9 25.6 26.8 28.2 AUG 27.0 27.6 25.6 25.5 25.4 25.8 26.9 26.3 26.1 SEP SEP OCT 21.2 24.9 26.6 25.3 24.3 25.3 24.1 25.2 25.8 OCT 21.8 20.5 20.8 20.9 20.8 20.0 19.2 14.3 12.4 NOV 8.6 5.3 6.6 7.3 6.1 7.9 6.8 9.6 10.7 NOV 17.0 18.0 16.2 17.2 17.1 18.0 18.3 19.7 18.6 DEC 9.5 8.3 9.2 9.6 7.7 10.2 9.7 10.7 10.9 DEC 10.7 12.0 10.6 11.6 11.9 7.2 7.2 7.9 7.0 mean 15.7 15.2 16.3 15.9 15.3 16.2 15.4 17.2 17.8 mean 17.9 17.4 17.2 17.6 17.6 17.1 17.6 17.4 16.6 std dev 7.6 9.1 8.8 8.8 9.2 8.1 8.1 7.9 8.0 std dev 7.0 6.6 6.4 6.1 6.2 7.4 7.5 6.7 6.7 median 12.6 12.0 13.4 13.2 13.3 13.8 12.3 16.8 16.4 median 18.1 18.0 16.2 17.2 17.1 18.0 18.3 17.8 17.1 max 26.0 26.8 26.6 26.8 27.3 26.6 25.8 28.5 28.2 max 27.0 27.6 25.6 25.5 25.4 26.4 27.7 26.3 26.1 min 5.7 3.1 4.5 3.6 3.2 5.2 4.2 8.1 7.5 min 6.8 5.5 7.4 7.3 6.5 5.0 5.8 6.2 6.6 24 Table 2.2 Salinity (psu) during 2018 at the Lower Cape Fear River Program estuarine stations. NAV HB BRR M61 M54 M35 M23 M18 NCF6 SC-CH JAN 0.3 3.1 6.3 9.9 11.2 16.4 23.5 26.4 0.1 0.8 FEB 0.1 0.1 0.1 0.8 2.7 8.3 24.2 27.7 0.2 0.1 MAR 0.1 0.6 0.5 3.9 6.3 14.8 21.1 31.4 0.5 1.4 APR 0.1 0.1 2.0 6.0 9.4 14.3 20.0 23.4 0.1 1.0 MAY 0.1 0.1 0.1 0.2 1.8 5.5 13.0 18.6 0.0 0.1 JUN 0.1 0.0 0.1 0.2 1.0 3.1 9.1 16.4 0.0 0.1 JUL 0.3 0.7 2.5 5.9 8.0 13.7 22.4 26.1 0.1 2.4 AUG 0.0 0.0 0.0 0.0 0.1 1.3 7.0 8.2 0.0 0.1 SEP OCT 0.0 0.0 0.0 1.0 1.2 3.0 8.1 10.7 0.0 0.1 NOV 0.1 0.6 0.2 2.1 4.8 10.8 16.8 29.6 0.5 0.1 DEC 0.0 0.1 0.1 1.1 3.0 8.7 11.9 26.5 0.0 0.1 mean 0.1 0.5 1.1 2.8 4.5 9.1 16.1 22.3 0.1 0.6 std dev 0.1 0.9 1.9 3.2 3.7 5.3 6.5 7.7 0.2 0.8 median 0.1 0.1 0.1 1.1 3.0 8.7 16.8 26.1 0.1 0.1 max 0.3 3.1 6.3 9.9 11.2 16.4 24.2 31.4 0.5 2.4 min 0.0 0.0 0.0 0.0 0.1 1.3 7.0 8.2 0.0 0.1 25 0 5 10 15 20 25 30 NAV HB BRR M61 M54 M35 M23 M18 NCF6 SC-CH Sa l i n t y ( P S U ) Figure 2.1 Salinity at the Lower Cape Fear River Program estuarine stations 1995-2017 versus 2018. 1995-2017 2018 26 Table 2.3 Conductivity (mS/cm) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP BBT IC NCF6 JAN 0.67 5.83 11.29 17.09 19.19 27.15 37.85 42.01 JAN 0.15 0.33 0.27 0.15 0.22 0.18 FEB 0.15 0.18 0.22 1.51 4.99 14.29 38.25 43.12 FEB 0.15 0.18 0.18 0.17 0.17 0.31 MAR 0.16 1.23 0.92 7.08 11.06 24.24 33.72 48.16 MAR 0.12 0.13 0.14 0.13 0.14 0.95 APR 0.14 0.24 3.71 10.60 15.86 23.62 32.03 36.94 APR 0.12 0.11 0.14 0.12 0.13 0.13 MAY 0.17 0.17 0.18 0.41 3.52 9.78 21.57 30.13 MAY 0.10 0.10 0.10 0.09 0.09 0.10 JUN 0.11 0.09 0.14 0.49 2.00 5.81 15.60 26.78 JUN 0.10 0.12 0.15 0.09 0.11 0.07 JUL 0.64 1.41 4.76 10.57 13.96 23.46 35.53 40.94 JUL 0.14 0.13 0.22 0.18 0.16 0.12 AUG 0.09 0.09 0.10 0.10 0.16 2.51 12.02 14.22 AUG 0.07 0.09 0.08 0.09 0.01 0.07 SEP SEP 0.13 0.24 0.16 0.12 0.17 2.75 OCT 0.09 0.09 0.09 2.02 2.26 5.61 13.95 18.19 OCT 0.08 0.08 0.08 0.07 0.07 0.07 NOV 0.10 1.20 0.43 3.90 8.64 18.19 27.07 45.52 NOV 0.10 0.11 0.12 0.11 0.11 1.02 DEC 0.09 0.21 0.12 2.10 5.86 15.04 19.95 41.44 DEC 0.06 0.07 0.07 0.07 0.07 0.08 mean 0.22 0.98 2.00 5.08 7.95 15.43 26.14 35.22 mean 0.11 0.14 0.14 0.11 0.12 0.49 std dev 0.22 1.69 3.48 5.56 6.30 8.62 9.92 11.34 std dev 0.03 0.08 0.06 0.04 0.06 0.79 median 0.14 0.21 0.22 2.10 5.86 15.04 27.07 40.94 median 0.11 0.12 0.14 0.11 0.12 0.12 max 0.67 5.83 11.29 17.09 19.19 27.15 38.25 48.16 max 0.15 0.33 0.27 0.18 0.22 2.75 min 0.09 0.09 0.09 0.10 0.16 2.51 12.02 14.22 min 0.06 0.07 0.07 0.07 0.01 0.07 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SRWC 6RC LCO GCO SR BRN HAM JAN 0.09 0.16 0.15 0.56 0.46 0.12 0.15 0.09 1.61 JAN 0.10 0.06 0.08 0.16 0.10 0.13 0.09 0.16 0.14 FEB 0.08 0.16 0.20 0.40 0.85 0.12 0.14 0.11 0.26 FEB 0.10 0.06 0.08 0.15 0.11 0.13 0.10 0.16 0.18 MAR 0.08 0.18 0.16 0.57 0.50 0.12 0.14 0.14 2.62 MAR 0.10 0.06 0.07 0.14 0.09 0.13 0.08 0.14 0.16 APR 0.11 0.13 0.13 0.34 0.51 0.11 0.12 0.13 1.84 APR 0.09 0.06 0.07 0.11 0.08 0.15 0.08 0.11 0.10 MAY 0.09 0.15 0.13 0.38 1.75 0.11 0.11 0.08 0.12 MAY 0.09 0.06 0.08 0.15 0.10 0.17 0.09 0.14 0.17 JUN 0.07 0.18 0.16 0.30 1.24 0.09 0.11 0.08 0.07 JUN 0.08 0.06 0.07 0.13 0.10 0.13 0.08 0.12 0.13 JUL 0.07 0.30 0.28 1.59 4.56 0.16 0.18 0.13 4.52 JUL 0.09 0.06 0.09 0.16 0.12 0.36 0.11 0.13 0.16 AUG 0.07 0.15 0.14 0.63 2.43 0.15 0.19 0.11 0.26 AUG 0.08 0.06 0.06 0.09 0.08 0.10 0.06 0.14 0.11 SEP SEP OCT 0.06 0.16 0.15 0.45 0.87 0.13 0.12 0.09 0.17 OCT 0.09 0.06 0.07 0.13 0.10 0.15 0.08 0.12 0.18 NOV 0.07 0.16 0.14 0.39 0.47 0.12 0.14 0.10 0.15 NOV 0.10 0.05 0.07 0.14 0.10 0.15 0.07 0.12 0.17 DEC 0.08 0.14 0.13 0.33 0.44 0.12 0.13 0.11 0.11 DEC 0.07 0.05 0.06 0.11 0.08 0.10 0.06 0.10 0.14 mean 0.08 0.17 0.16 0.54 1.28 0.12 0.14 0.11 1.06 mean 0.09 0.06 0.07 0.13 0.10 0.16 0.08 0.13 0.15 std dev 0.01 0.05 0.04 0.36 1.26 0.02 0.03 0.02 1.45 std dev 0.01 0.00 0.01 0.02 0.01 0.07 0.02 0.02 0.03 median 0.08 0.16 0.15 0.40 0.85 0.12 0.14 0.11 0.26 median 0.09 0.06 0.07 0.14 0.10 0.13 0.08 0.13 0.16 max 0.11 0.30 0.28 1.59 4.56 0.16 0.19 0.14 4.52 max 0.10 0.06 0.09 0.16 0.12 0.36 0.11 0.16 0.18 min 0.06 0.13 0.13 0.30 0.44 0.09 0.11 0.08 0.07 min 0.07 0.05 0.06 0.09 0.08 0.10 0.06 0.10 0.10 27 Table 2.4 pH during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP BBT IC NCF6 JAN 7.5 7.4 7.5 7.6 7.8 8.0 8.1 8.1 JAN 6.4 6.9 6.9 6.4 6.8 6.7 FEB 6.4 6.5 7.0 6.8 7.3 7.7 8.1 8.1 FEB 6.2 6.6 6.7 6.7 6.7 6.4 MAR 7.0 7.1 7.1 7.1 7.5 7.9 8.0 7.7 MAR 6.8 6.9 6.9 6.8 6.9 6.8 APR 6.9 6.9 6.9 7.1 7.3 7.7 8.0 8.0 APR 6.9 6.9 6.8 6.7 6.8 6.8 MAY 6.7 6.7 6.7 6.6 6.7 7.0 7.5 7.7 MAY 6.7 6.7 6.7 6.4 6.5 6.3 JUN 6.4 6.1 6.5 6.2 6.6 6.7 7.1 7.6 JUN 6.5 6.6 6.7 6 6.4 5.8 JUL 6.5 6.8 6.8 7.0 7.1 7.5 7.9 8.0 JUL 6.7 6.7 6.9 6.6 6.6 5.9 AUG 6.2 6.3 6.3 6.0 6.3 6.5 6.9 7.0 AUG 6.2 6.2 6.2 6.1 6.2 5.7 SEP SEP 6.7 7.0 6.7 6.4 6.6 6.4 OCT 6.4 6.5 6.7 6.6 6.8 7.0 7.1 7.4 OCT 6.4 6.2 6.1 5.8 5.9 5.5 NOV 6.9 7.3 7.2 7.5 7.4 7.8 8.0 8.0 NOV 6.5 6.5 6.7 6.7 6.7 6.6 DEC 7.1 7.1 7.6 7.0 7.6 7.8 7.9 8.0 DEC 6.1 5.8 5.7 5.5 5.6 5.7 mean 6.7 6.8 6.9 6.9 7.1 7.4 7.7 7.8 mean 6.5 6.6 6.6 6.3 6.5 6.2 std dev 0.4 0.4 0.4 0.5 0.5 0.5 0.5 0.3 std dev 0.3 0.4 0.4 0.4 0.4 0.5 median 6.7 6.8 6.9 7.0 7.3 7.7 7.9 8.0 median 6.5 6.7 6.7 6.4 6.6 6.4 max 7.5 7.4 7.6 7.6 7.8 8.0 8.1 8.1 max 6.9 7.0 6.9 6.8 6.9 6.8 min 6.2 6.1 6.3 6.0 6.3 6.5 6.9 7.0 min 6.1 5.8 5.7 5.5 5.6 5.5 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SRWC 6RC LCO GCO SR BRN HAM JAN 4.9 6.4 6.7 6.6 6.6 6.5 6.1 5.9 6.3 JAN 5.5 3.6 5.6 6.3 5.9 6.0 5.8 6.4 6.4 FEB 5.2 6.5 6.4 6.7 6.6 6.7 6.5 5.6 6.2 FEB 5.4 3.9 5.3 6.3 6.3 6.3 6.0 6.6 6.8 MAR 5.3 6.4 6.9 6.5 6.7 6.9 6.5 6.6 6.5 MAR 6.8 4.0 6.1 6.9 6.7 6.7 6.5 5.8 6.2 APR 6.1 6.7 7.0 6.6 6.8 7.0 6.8 6.9 7.2 APR 6.3 4.1 5.9 6.6 6.2 6.6 6.3 6.7 6.5 MAY 6.1 6.7 6.8 6.8 6.6 7.1 6.7 6.1 6.4 MAY 6.3 4.0 6.3 7.1 6.9 6.9 6.3 7.1 7.1 JUN 4.8 6.7 6.7 6.4 6.6 6.8 6.6 6.0 6.1 JUN 6.1 4.0 5.9 6.8 6.7 6.6 6.2 6.9 6.8 JUL 6 6.8 7.0 7.0 7.0 7.7 7.0 6.5 6.4 JUL 6.1 4.0 6.3 7.0 6.9 7.0 6.7 6.7 7.0 AUG 5.7 6.4 6.5 6.6 6.7 7.1 6.9 6.2 6.9 AUG 6.0 3.9 5.7 6.3 5.7 6.2 5.9 6.9 6.6 SEP SEP OCT 4.3 6.4 6.7 6.6 6.6 6.9 6.4 6.0 6.3 OCT 6.0 4.1 5.9 6.6 6.5 6.4 6.2 6.3 6.6 NOV 4.8 6.4 6.8 6.6 6.6 6.3 6.4 5.6 6.3 NOV 6.0 4.1 5.9 6.6 6.5 6.6 6.3 6.7 6.9 DEC 5.0 6.5 6.8 6.6 6.7 6.7 6.5 5.9 6.3 DEC 5.4 3.5 5.1 5.8 5.7 6.6 6.5 6.3 7.0 mean 5.3 6.5 6.8 6.6 6.7 6.9 6.6 6.1 6.4 mean 6.0 3.9 5.8 6.6 6.4 6.5 6.2 6.6 6.7 std dev 0.6 0.2 0.2 0.2 0.1 0.4 0.3 0.4 0.3 std dev 0.4 0.2 0.4 0.4 0.4 0.3 0.3 0.4 0.3 median 5.2 6.5 6.8 6.6 6.6 6.9 6.5 6.0 6.3 median 6.0 4.0 5.9 6.6 6.5 6.6 6.3 6.7 6.8 max 6.1 6.8 7.0 7.0 7.0 7.7 7.0 6.9 7.2 max 6.8 4.1 6.3 7.1 6.9 7.0 6.7 7.1 7.1 min 4.3 6.4 6.4 6.4 6.6 6.3 6.1 5.6 6.1 min 5.4 3.5 5.1 5.8 5.7 6.0 5.8 5.8 6.2 28 Table 2.5 Dissolved Oxygen (mg/l) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP BBT IC NCF6 JAN 12.7 12.3 12.1 12.1 12.2 12.2 12.0 11.8 JAN 12.9 12.6 12.5 13.6 12.9 12.3 FEB 10.5 9.9 10.3 9.1 10.2 10.4 10.3 10.2 FEB 11.5 11.4 11.5 11.3 11.5 10.0 MAR 9.1 9.0 9.1 8.8 9.1 8.9 9.2 8.3 MAR 10.2 10.1 10.0 9.8 9.7 8.6 APR 7.5 7.4 7.3 7.3 7.3 7.6 8.2 8.3 APR 9.2 8.9 8.3 7.9 8.4 6.6 MAY 5.1 4.9 4.9 4.6 4.8 5.6 6.5 6.4 MAY 7.5 7.2 7.1 5.7 6.2 5.0 JUN 4.4 3.5 5.0 3.7 4.7 4.5 5.2 5.5 JUN 5.9 5.5 5.2 3.7 4.4 2.8 JUL 3.8 4.3 5.2 4.5 4.5 5.5 6.1 6.1 JUL 6.1 5.8 5.9 5.4 5.3 4.2 AUG 4.8 5.1 5.1 4.1 4.0 4.0 4.6 4.7 AUG 6.2 6.0 5.8 4.8 5.1 3.4 SEP SEP 5.4 4.9 4.2 3.5 3.6 3.6 OCT 4.8 4.4 4.2 3.8 4.3 4.5 5.2 5.9 OCT 4.9 4.5 2.6 0.2 0.2 0.2 NOV 7.5 7.5 7.6 7.2 7.8 8.2 8.2 7.4 NOV 8.8 8.8 8.6 8.3 8.0 6.8 DEC 9.4 9.4 9.5 9.5 9.7 9.6 9.7 8.6 DEC 10.1 9.9 9.6 8.8 9.1 8.3 mean 7.2 7.1 7.3 6.8 7.1 7.4 7.7 7.6 mean 8.2 8.0 7.6 6.9 7.0 6.0 std dev 2.9 2.8 2.6 2.8 2.9 2.7 2.4 2.1 std dev 2.6 2.7 3.0 3.7 3.6 3.4 median 7.5 7.4 7.3 7.2 7.3 7.6 8.2 7.4 median 8.2 8.0 7.7 6.8 7.1 5.8 max 12.7 12.3 12.1 12.1 12.2 12.2 12.0 11.8 max 12.9 12.6 12.5 13.6 12.9 12.3 min 3.8 3.5 4.2 3.7 4.0 4.0 4.6 4.7 min 4.9 4.5 2.6 0.2 0.2 0.2 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SRWC 6RC LCO GCO SR BRN HAM JAN 10.0 12.8 13.8 11.7 12.2 13.6 12.3 9.0 10.6 JAN 10.8 9.8 10.5 10.3 10.4 10.8 10.9 12.1 11.5 FEB 9.0 10.1 10.7 9.9 9.5 11.4 9.7 9.8 9.3 FEB 8.7 7.1 8.4 8.9 8.6 7.7 8.6 10.3 10.2 MAR 11.0 10.8 11.9 11.7 11.4 12.2 11.1 8.4 9.8 MAR 9.6 9.1 9.9 10.0 10.1 9.4 10.4 9.1 9.5 APR 8.6 8.4 10.1 8.5 8.8 10.1 8.7 6.6 7.5 APR 6.5 6.3 7.6 7.6 7.4 6.3 5.1 8.0 7.7 MAY 7.0 6.5 7.7 6.4 3.9 8.8 7.5 4.9 5.2 MAY 5.7 6.3 6.9 7.8 7.7 6.9 2.3 7.9 6.4 JUN 4.9 5.9 5.4 4.6 4.4 7.4 6.4 3.0 3.5 JUN 4.7 5.1 5.9 6.6 6.8 5.7 4.6 7.2 6.5 JUL 3.4 7.1 1.7 3.1 6.6 8.8 4.6 3.2 3.5 JUL 4.4 5.2 4.7 6.4 5.6 5.6 5.4 7.2 5.9 AUG 4.1 4.9 2.0 2.6 4.7 6.7 5.1 3.3 4.0 AUG 4.1 4.6 5.6 5.0 5.4 4.8 3.7 6.9 6.1 SEP SEP OCT 1.7 3.7 3.5 6.5 5.9 7.1 4.5 0.2 0.3 OCT 3.2 3.6 5.5 6.8 6.5 6.1 4.6 9.2 8.2 NOV 8.9 11.1 12.1 10.8 10.6 11.6 10.5 7.5 8.1 NOV 6.0 5.4 7.5 8.1 8.0 7.3 5.8 7.8 6.7 DEC 8.8 9.8 10.9 9.9 10.2 10.9 9.2 8.0 8.4 DEC 9.0 7.4 8.9 8.5 9.0 11.7 9.8 11.0 11.0 mean 7.0 8.3 8.2 7.8 8.0 9.9 8.1 5.8 6.4 mean 6.6 6.4 7.4 7.8 7.8 7.5 6.5 8.8 8.2 std dev 3.0 2.9 4.3 3.3 3.0 2.3 2.7 3.1 3.3 std dev 2.5 1.9 1.9 1.6 1.7 2.2 2.9 1.7 2.1 median 8.6 8.4 10.1 8.5 8.8 10.1 8.7 6.6 7.5 median 6.0 6.3 7.5 7.8 7.7 6.9 5.4 8.0 7.7 max 11.0 12.8 13.8 11.7 12.2 13.6 12.3 9.8 10.6 max 10.8 9.8 10.5 10.3 10.4 11.7 10.9 12.1 11.5 min 1.7 3.7 1.7 2.6 3.9 6.7 4.5 0.2 0.3 min 3.2 3.6 4.7 5.0 5.4 4.8 2.3 6.9 5.9 29 0 1 2 3 4 5 6 7 8 9 NC11 AC DP IC NAV HB BRR M61 M54 M35 M23 M18 NCF117 NCF6 B210 BBT Di s s o l v e d O x y g e n ( m g / L ) Figure 2.2 Dissolved Oxygen at the Lower Cape Fear River Program mainstem stations 1995-2017 versus 2018. 1995-2017 2018 30 Table 2.6 Field Turbidity (NTU) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP BBT IC NCF6 JAN 12 7 5 5 4 5 3 3 JAN 5 7 8 5 8 12 FEB 21 24 21 21 21 10 13 17 FEB 87 85 40 30 22 10 MAR MAR 20 18 18 10 14 6 APR 11 13 11 6 5 4 3 6 APR 17 13 11 9 10 6 MAY 9 7 7 7 9 8 5 5 MAY 28 28 29 9 13 5 JUN 7 4 7 5 7 6 3 3 JUN 13 12 13 5 7 3 JUL 8 5 6 3 3 3 2 2 JUL 15 19 11 9 7 6 AUG 22 14 13 8 14 8 4 4 AUG 30 26 24 13 15 5 SEP SEP 17 16 14 8 14 22 OCT 15 13 13 10 11 9 4 4 OCT 22 21 13 5 9 6 NOV 17 14 17 12 10 4 4 10 NOV 23 19 14 11 7 7 DEC 7 6 7 4 12 3 2 9 DEC 30 30 28 9 17 4 mean 13 11 11 8 10 6 4 6 mean 26 25 19 10 12 8 std dev 6 6 5 5 5 3 3 5 std dev 21 20 10 7 5 5 median 12 10 9 7 10 6 4 5 median 21 19 14 9 12 6 max 22 24 21 21 21 10 13 17 max 87 85 40 30 22 22 min 7 4 5 3 3 3 2 2 min 5 7 8 5 7 3 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SRWC 6RC LCO GCO SR BRN HAM JAN 10 3 1 2 5 13 7 18 11 JAN 5 6 5 13 10 3 8 8 20 FEB 10 2 2 5 10 10 6 6 14 FEB 3 3 2 7 3 2 3 6 6 MAR 9 4 2 8 12 6 10 6 11 MAR 2 4 1 5 3 2 5 8 7 APR 9 3 2 4 12 6 9 3 7 APR 4 5 8 22 13 4 23 13 22 MAY 13 5 2 4 9 4 6 5 9 MAY 5 7 3 5 4 4 18 6 7 JUN 10 8 3 6 5 7 10 4 8 JUN 4 7 4 7 6 4 11 5 6 JUL 9 2 16 3 25 4 5 4 4 JUL 3 8 7 2 2 3 10 4 6 AUG 7 4 3 3 8 5 8 4 10 AUG 2 15 3 4 5 3 5 4 5 SEP SEP OCT 3 4 4 3 5 5 5 3 5 OCT 2 2 2 7 3 3 3 6 4 NOV 6 2 1 2 6 4 3 3 7 NOV 1 2 0 3 1 1 1 4 3 DEC 4 0 0 0 4 1 0 0 7 DEC 1 0 0 1 0 0 0 4 3 mean 8 3 3 4 9 6 6 5 8 mean 3 5 3 7 5 3 8 6 8 std dev 3 2 4 2 6 3 3 5 3 std dev 1 4 3 6 4 1 7 3 7 median 9 3 2 3 8 5 6 4 8 median 3 5 3 5 3 3 5 6 6 max 13 8 16 8 25 13 10 18 14 max 5 15 8 22 13 4 23 13 22 min 3 0 0 0 4 1 0 0 4 min 1 0 0 1 0 0 0 4 3 31 0 5 10 15 20 25 30 NC11 AC DP IC NAV HB BRR M61 M54 M35 M23 M18 NCF117 NCF6 B210 BBT Fi e l d T u r b i d i t y ( N T U ) Figure 2.3 Field Turbidity at the Lower Cape Fear River Program mainstem stations, 1995-2017 versus 2018. 1995-2017 2018 32 Table 2.7 Total Suspended Solids (mg/L) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP IC NCF6 JAN 10.8 9.7 8.0 7.6 7.4 9.9 20.3 13.4 JAN 1.4 4.5 5.8 5.8 9.3 FEB 10.6 11.4 11.0 15.8 22.9 11.2 28.9 36.0 FEB 85.0 72.5 38.4 17.2 10.4 MAR 7.3 7.2 12.6 11.6 15.0 15.6 41.4 74.4 MAR 27.3 23.3 23.9 15.1 9.4 APR 10.5 11.6 12.1 9.0 8.5 9.9 11.0 17.4 APR 13.0 8.7 5.6 6.5 7.7 MAY 6.5 5.4 5.0 9.3 12.1 12.4 10.6 12.7 MAY 29.9 30.5 29.9 5.8 4.8 JUN 4.6 4.3 5.4 4.4 9.0 6.6 6.9 7.9 JUN 9.0 8.6 10.7 3.9 3.7 JUL 5.8 4.1 8.9 7.4 6.8 8.0 9.5 9.0 JUL 16.6 16.0 14.3 10.5 10.2 AUG 27.0 9.1 9.9 9.9 13.3 8.7 7.7 9.4 AUG 53.2 36.4 46.2 28.6 6.0 SEP SEP 13.5 8.8 10.9 9.7 41.1 OCT 15.8 8.6 6.9 8.1 8.7 8.8 7.0 8.0 OCT 23.6 27.4 13.3 13.0 14.3 NOV 8.1 8.6 9.6 10.1 11.4 9.1 9.3 24.9 NOV 27.0 21.0 14.8 4.1 7.2 DEC 6.4 5.4 6.2 5.2 17.4 7.1 7.0 39.2 DEC 28.4 28.7 25.0 15.0 1.4 mean 10.3 7.8 8.7 8.9 12.0 9.8 14.5 22.9 mean 27.3 23.9 19.9 11.3 11.3 std dev 6.4 2.7 2.6 3.1 4.9 2.6 11.2 20.3 std dev 22.4 18.4 13.0 7.1 7.1 median 8.1 8.6 8.9 9.0 11.4 9.1 9.5 13.4 median 25.3 22.2 14.6 10.9 10.1 max 27.0 11.6 12.6 15.8 22.9 15.6 41.4 74.4 max 85.0 72.5 46.2 6.8 28.6 min 4.6 4.1 5.0 4.4 6.8 6.6 6.9 7.9 min 1.4 4.5 5.6 10.6 3.9 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SRWC 6RC LCO GCO SR BRN HAM JAN 1.4 3.0 6.6 6.6 5.1 7.7 9.3 JAN 1.8 1.4 FEB 3.4 3.5 5.7 9.5 6.2 3.5 10.2 FEB 1.3 1.4 MAR 1.4 5.1 6.7 1.4 5.6 3.1 12.7 MAR 1.4 1.5 APR 3.4 4.3 5.2 3.8 13.0 4.2 11.7 APR 3.5 5.8 MAY 5.9 6.1 5.9 1.4 7.8 5.4 13.0 MAY 4.2 3.7 JUN 9.9 6.3 6.0 7.5 12.9 2.9 15.6 JUN 3.9 5.4 JUL 1.3 5.8 10.8 1.3 19.0 7.0 7.2 JUL 1.3 1.3 AUG 9.0 4.0 7.3 1.5 4.1 5.5 15.8 AUG 4.2 4.1 SEP SEP OCT 6.2 8.3 6.3 4.0 6.6 16.1 13.3 OCT 1.4 4.3 NOV 1.3 1.3 2.9 1.3 1.3 1.4 5.6 NOV 1.3 1.3 DEC 1.5 1.3 1.4 1.4 1.3 1.3 7.8 DEC 1.3 1.8 mean 4.1 4.5 5.9 3.6 7.5 5.3 11.1 mean 2.3 2.9 std dev 3.2 2.2 2.4 3.0 5.4 4.1 3.4 std dev 1.3 1.8 median 3.4 4.3 6.0 1.5 6.2 4.2 11.7 median 1.4 1.8 max 9.9 8.3 10.8 9.5 19.0 16.1 15.8 max 4.2 5.8 min 1.3 1.3 1.4 1.3 1.3 1.3 5.6 min 1.3 1.3 33 Table 2.8 Light Attenuation (k) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP BBT IC NCF6 JAN JAN FEB 3.55 4.54 4.37 3.62 4.01 2.20 2.19 2.44 FEB 7.10 6.42 4.52 3.64 3.05 4.22 MAR MAR 3.23 3.45 3.08 2.95 2.68 4.16 APR 3.75 3.37 3.06 2.49 2.55 1.96 1.41 1.11 APR 2.83 2.67 2.90 2.79 2.91 3.46 MAY 3.01 2.75 3.65 2.25 1.96 MAY 4.02 4.23 3.63 3.74 3.58 3.90 JUN 4.06 4.09 3.20 3.95 3.96 3.54 2.53 2.74 JUN 2.60 2.99 3.31 4.20 3.30 3.86 JUL 3.33 3.12 3.66 3.09 2.52 1.73 1.90 JUL 2.67 2.65 2.87 AUG 4.56 4.02 3.48 2.71 4.62 3.72 3.31 3.24 AUG 4.78 4.75 4.36 3.78 4.47 4.58 SEP SEP 1.00 1.13 1.44 1.02 1.33 1.03 OCT OCT 1.72 1.76 0.74 0.75 1.54 1.31 NOV NOV DEC DEC mean 3.98 3.73 3.33 3.29 3.65 2.79 2.24 2.23 mean 3.33 3.34 2.98 2.86 2.86 3.32 std dev 0.44 0.58 0.56 0.64 0.73 0.80 0.66 0.74 std dev 1.80 1.61 1.24 1.30 1.03 1.36 max 4.56 4.54 4.37 3.95 4.62 3.72 3.31 3.24 max 7.10 6.42 4.52 4.20 4.47 4.58 min 3.55 3.01 2.75 2.49 2.55 1.96 1.41 1.11 min 1.00 1.13 0.74 0.75 1.33 1.03 34 Table 2.9 Total Nitrogen (mg/l) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP IC NCF6 JAN 1,630 1,090 940 950 890 580 370 510 JAN 1,870 1,880 1,750 1,350 1,070 FEB 1,630 1,920 1,620 1,550 1,600 1,020 680 850 FEB 2,330 2,190 1,880 1,730 1,200 MAR 1,360 1,290 1,400 1,240 1,200 1,330 580 920 MAR 1,700 1,590 1,430 1,330 950 APR 1,200 1,190 1,290 1,050 830 730 480 630 APR 1,510 1,480 1,620 1,330 1,110 MAY 760 1,540 1,530 1,120 1,100 900 650 680 MAY 1,370 1,330 1,630 1,890 1,790 JUN 1,050 1,230 1,190 1,080 1,140 1,070 760 780 JUN 1,160 1,040 1,150 970 670 JUL 890 740 440 350 420 210 50 50 JUL 1,470 1,640 1,480 1,480 840 AUG 970 930 1,030 950 970 920 770 650 AUG 1,580 1,080 1,130 980 1,000 SEP SEP 1,860 1,980 1,460 1,530 1,320 OCT 1,960 2,020 1,930 1,760 1,480 1,340 1,140 780 OCT 1,110 910 1,010 1,010 1,700 NOV 1,610 1,390 1,480 1,400 1,330 1,350 1,280 1,260 NOV 1,390 1,640 1,640 1,670 1,470 DEC 820 900 910 810 780 830 580 120 DEC 1,060 880 940 1,070 970 mean 1,262 1,295 1,251 1,115 1,067 935 667 657 mean 1,534 1,470 1,427 1,362 1,174 std dev 401 405 409 380 338 350 337 344 std dev 367 430 304 311 340 median 1,200 1,230 1,290 1,080 1,100 920 650 680 median 1,490 1,535 1,470 1,340 1,090 max 1,960 2,020 1,930 1,760 1,600 1,350 1,280 1,260 max 2,330 2,190 1,880 1,890 1,790 min 760 740 440 350 420 210 50 50 min 1,060 880 940 970 670 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SR-WC 6RC LCO GCO SR BRN HAM JAN 3,000 1,900 670 2,520 2,020 2,360 2,070 1,410 950 JAN 950 1,040 1,140 2,150 1,630 1,030 820 1,570 2,030 FEB 1,480 1,120 1,940 3,960 4,100 1,650 2,050 1,220 700 FEB 870 820 480 1,550 1,320 850 550 1,280 1,260 MAR 2,240 1,230 680 2,310 1,680 960 1,170 1,490 1,010 MAR 820 1,000 590 1,370 1,280 820 850 920 840 APR 2,130 1,430 930 2,950 2,270 1,350 1,630 1,440 820 APR 850 800 720 2,040 1,090 1,220 1,130 930 1,350 MAY 720 1,230 930 2,540 1,360 2,200 1,510 1,600 1,880 MAY 970 1,100 950 2,030 960 1,580 1,540 1,080 900 JUN 1,120 1,290 630 1,600 960 1,370 1,280 870 910 JUN 890 1,100 830 1,640 850 1,040 1,170 870 870 JUL 1,630 910 1,300 1,270 800 350 6,480 1,140 800 JUL 1,230 1,940 900 960 960 1,300 1,310 1,060 1,560 AUG 1,690 1,140 1,100 1,280 2,490 1,210 2,890 1,160 1,010 AUG 1,440 2,140 1,590 1,400 1,410 860 1,450 1,280 1,430 SEP SEP OCT 2,530 1,520 1,640 2,970 2,110 1,170 2,140 2,750 1,840 OCT 1,530 2,020 1,560 2,310 1,350 1,480 1,110 1,750 910 NOV 1,820 1,550 960 4,540 4,820 1,290 1,790 1,090 710 NOV 1,280 1,900 1,250 1,790 1,400 1,360 1,050 1,740 970 DEC 1,130 1,170 950 4,900 5,430 1,260 1,850 1,450 1,070 DEC 1,470 750 950 2,560 1,840 1,420 660 1,630 1,820 mean 1,772 1,317 1,066 2,804 2,549 1,379 2,260 1,420 1,064 mean 1,118 1,328 996 1,800 1,281 1,178 1,058 1,283 1,267 std dev 670 269 413 1,237 1,555 551 1,476 490 412 std dev 276 548 361 470 300 272 314 339 412 median 1,690 1,230 950 2,540 2,110 1,290 1,850 1,410 950 median 970 1,100 950 1,790 1,320 1,220 1,110 1,280 1,260 max 3,000 1,900 1,940 4,900 5,430 2,360 6,480 2,750 1,880 max 1,530 2,140 1,590 2,560 1,840 1,580 1,540 1,750 2,030 min 720 910 630 1,270 800 350 1,170 870 700 min 820 750 480 960 850 820 550 870 840 35 Table 2.10 Nitrate/Nitrite (mg/l) during 2018 at the Lower Cape Fear River stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP IC NCF6 JAN 630 490 440 350 390 280 170 110 JAN 1,170 980 850 650 270 FEB 630 620 620 550 500 420 180 50 FEB 930 1,090 980 930 400 MAR 660 590 600 540 500 330 80 20 MAR 700 690 730 630 250 APR 500 490 490 350 330 230 180 130 APR 710 680 520 530 210 MAY 760 640 630 320 400 300 250 180 MAY 570 530 530 490 390 JUN 450 330 490 380 440 370 260 180 JUN 660 640 650 470 270 JUL 690 640 440 350 320 210 90 50 JUL 870 1,040 880 860 140 AUG 370 430 430 250 270 220 170 150 AUG 480 480 430 380 200 SEP SEP 1,060 880 760 530 220 OCT 760 720 630 560 580 440 240 180 OCT 410 410 310 110 10 NOV 610 490 580 500 430 350 280 60 NOV 690 740 740 670 470 DEC 420 400 410 410 380 330 380 120 DEC 560 580 540 570 570 mean 589 531 524 415 413 316 207 112 mean 734 728 660 568 283 std dev 135 120 89 106 90 77 87 59 std dev 231 224 199 213 152 median 630 490 490 380 400 330 180 120 median 695 685 690 550 260 max 760 720 630 560 580 440 380 180 max 1,170 1,090 980 930 570 min 370 330 410 250 270 210 80 20 min 410 410 310 110 10 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SR-WC 6RC LCO GCO SR BRN HAM JAN 1,200 900 170 1,820 1,320 1,260 1,170 510 250 JAN 250 40 440 1,050 730 330 120 770 930 FEB 280 520 1,040 3,160 3,200 1,050 1,050 520 300 FEB 370 20 80 850 720 250 50 680 560 MAR 240 530 80 1,510 880 260 370 290 210 MAR 220 10 90 670 480 220 50 320 240 APR 630 330 30 1,650 970 250 430 240 120 APR 250 10 220 1,140 490 520 230 230 550 MAY 120 330 30 1,340 160 500 610 300 380 MAY 270 10 250 1,130 360 680 340 480 200 JUN 220 690 30 1,000 360 670 580 270 210 JUN 290 10 230 1,040 350 340 370 370 370 JUL 130 210 10 270 10 350 2,280 240 200 JUL 230 40 400 460 260 500 110 460 160 AUG 90 140 10 380 990 410 1,690 160 110 AUG 140 40 90 300 210 60 50 480 230 SEP SEP OCT 30 220 40 1,670 1,010 170 540 50 40 OCT 330 20 460 1,210 450 480 110 950 310 NOV 220 850 360 3,740 3,920 690 1,090 290 210 NOV 380 10 250 590 400 360 50 840 170 DEC 230 570 350 3,900 4,430 560 950 450 370 DEC 870 50 350 1,860 1,340 920 160 830 1,120 mean 308 481 195 1,858 1,568 561 978 302 218 mean 327 24 260 936 526 424 149 583 440 std dev 334 259 309 1,238 1,543 342 584 143 105 std dev 193 16 139 431 316 235 116 241 323 median 220 520 40 1,650 990 500 950 290 210 median 270 20 250 1,040 450 360 110 480 310 max 1,200 900 1,040 3,900 4,430 1,260 2,280 520 380 max 870 50 460 1,860 1,340 920 370 950 1,120 min 30 140 10 270 10 170 370 50 40 min 140 10 80 300 210 60 50 230 160 36 0 100 200 300 400 500 600 700 800 NC11 AC DP IC NAV HB BRR M61 M54 M35 M23 M18 NCF117 NCF6 B210 Ni t r a t e + N i t r i t e ( mg/ L ) Figure 2.4 Nitrate + Nitrite at the Lower Cape Fear River Program mainstem stations, 1995-2017 versus 2018. 1995-2017 2018 37 Table 2.11 Ammonia (mg/l) during 2018 at the Lower Cape Fear River stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP IC NCF6 JAN 100 150 170 170 200 160 80 50 JAN 80 160 150 110 80 FEB 110 100 130 110 200 110 10 10 FEB 50 70 50 50 50 MAR 30 60 60 70 100 40 10 10 MAR 90 110 100 110 80 APR 90 100 90 90 90 80 60 10 APR 110 110 110 100 30 MAY 130 150 130 90 110 130 110 90 MAY 90 110 100 70 80 JUN 80 80 70 90 210 120 100 80 JUN 110 120 190 130 100 JUL 60 70 10 60 70 30 10 10 JUL 150 160 160 110 100 AUG 110 80 150 170 200 140 150 90 AUG 140 110 80 100 120 SEP SEP 80 280 140 100 50 OCT 140 140 160 230 200 210 190 200 OCT 50 40 70 50 170 NOV 90 90 130 110 120 60 10 170 NOV 90 80 90 90 100 DEC 80 60 150 90 120 60 10 120 DEC 90 50 60 20 30 mean 93 98 114 116 147 104 67 76 mean 94 117 108 87 83 std dev 31 34 50 52 54 55 64 67 std dev 30 64 43 32 40 median 90 90 130 90 120 110 60 80 median 90 110 100 100 80 max 140 150 170 230 210 210 190 200 max 150 280 190 130 170 min 30 60 10 60 70 30 10 10 min 50 40 50 20 30 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SR-WC 6RC LCO GCO SR BRN HAM JAN 70 60 40 90 160 90 270 80 110 JAN 30 20 20 70 10 10 10 40 40 FEB 180 20 10 70 130 80 320 40 130 FEB 10 40 10 60 20 20 10 70 280 MAR 40 30 10 70 60 70 120 50 50 MAR 40 70 50 60 50 40 40 90 120 APR 200 30 20 140 210 100 120 80 60 APR 20 40 10 170 50 60 240 40 120 MAY 130 80 40 130 1,240 300 190 120 160 MAY 180 400 70 100 80 100 620 100 260 JUN 240 60 40 110 290 390 70 170 110 JUN 60 280 40 60 40 100 90 50 60 JUL 230 70 50 200 70 60 10 80 130 JUL 80 340 70 30 50 70 320 70 50 AUG 170 80 50 100 80 60 110 580 100 AUG 130 170 90 90 90 80 90 130 120 SEP SEP OCT 540 260 310 170 180 200 380 320 380 OCT 170 570 150 110 110 90 100 140 70 NOV 330 10 10 120 50 50 30 30 30 NOV 80 240 70 70 70 60 50 100 40 DEC 450 100 30 130 140 240 240 100 130 DEC 30 90 30 130 10 10 10 100 80 mean 235 73 55 121 237 149 169 150 126 mean 75 205 55 86 53 58 144 85 113 std dev 153 68 86 40 340 116 121 164 93 std dev 60 178 41 39 33 34 187 34 84 median 200 60 40 120 140 90 120 80 110 median 60 170 50 70 50 60 90 90 80 max 540 260 310 200 1,240 390 380 580 380 max 180 570 150 170 110 100 620 140 280 min 40 10 10 70 50 50 10 30 30 min 10 20 10 30 10 10 10 40 40 38 0 5000 10000 15000 20000 25000 0 100 200 300 400 500 600 700 800 900 1000 20 1 0 20 1 1 20 1 2 20 1 3 20 1 4 20 1 5 20 1 6 20 1 7 20 1 8 Fe c a l C o l i f o r m B a c t e r i a ( C F U / 1 0 0 m L ) Am m o n i a ( m g / L ) Figure 2.5 Ammonia and Fecal Coliform Bacteria at COL 2010-2018. Ammonia Fecal 39 Table 2.12 Total Kjeldahl Nitrogen (mg/l) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP IC NCF6 JAN 1,000 600 500 600 500 300 200 400 JAN 700 900 900 700 800 FEB 1,000 1,300 1,000 1,000 1,100 600 500 800 FEB 1,400 1,100 900 800 800 MAR 700 700 800 700 700 1,000 500 900 MAR 1,000 900 700 700 700 APR 700 700 800 700 500 500 300 500 APR 800 800 1,100 800 900 MAY 50 900 900 800 700 600 400 500 MAY 800 800 1,100 1,400 1,400 JUN 600 900 700 700 700 700 500 600 JUN 500 400 500 500 400 JUL 200 100 50 50 100 50 50 50 JUL 600 600 600 600 700 AUG 600 500 600 700 700 700 600 500 AUG 1,100 600 700 600 800 SEP SEP 800 1,100 700 1,000 1,100 OCT 1,200 1,300 1,300 1,200 900 900 900 600 OCT 700 500 700 900 1,700 NOV 1,000 900 900 900 900 1,000 1,000 1,200 NOV 700 900 900 1,000 1,000 DEC 400 500 500 400 400 500 200 50 DEC 500 300 400 500 400 mean 677 764 732 705 655 623 468 555 mean 800 742 767 792 892 std dev 360 353 324 302 273 289 290 338 std dev 259 261 219 257 375 median 700 700 800 700 700 600 500 500 median 750 800 700 750 800 max 1,200 1,300 1,300 1,200 1,100 1,000 1,000 1,200 max 1,400 1,100 1,100 1,400 1,700 min 50 100 50 50 100 50 50 50 min 500 300 400 500 400 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SR-WC 6RC LCO GCO SR BRN HAM JAN 1,800 1,000 500 700 700 1,100 900 900 700 JAN 700 1,000 700 1,100 900 700 700 800 1,100 FEB 1,200 600 900 800 900 600 1,000 700 400 FEB 500 800 400 700 600 600 500 600 700 MAR 2,000 700 600 800 800 700 800 1,200 800 MAR 600 1,000 500 700 800 600 800 600 600 APR 1,500 1,100 900 1,300 1,300 1,100 1,200 1,200 700 APR 600 800 500 900 600 700 900 700 800 MAY 600 900 900 1,200 1,200 1,700 900 1,300 1,500 MAY 700 1,100 700 900 600 900 1,200 600 700 JUN 900 600 600 600 600 700 700 600 700 JUN 600 1,100 600 600 500 700 800 500 500 JUL 1,500 700 1,300 1,000 800 50 4,200 900 600 JUL 1,000 1,900 500 500 700 800 1,200 600 1,400 AUG 1,600 1,000 1,100 900 1,500 800 1,200 1,000 900 AUG 1,300 2,100 1,500 1,100 1,200 800 1,400 800 1,200 SEP SEP OCT 2,500 1,300 1,600 1,300 1,100 1,000 1,600 2,700 1,800 OCT 1,200 2,000 1,100 1,100 900 1,000 1,000 800 600 NOV 1,600 700 600 800 900 600 700 800 500 NOV 900 1,900 1,000 1,200 1,000 1,000 1,000 900 800 DEC 900 600 600 1,000 1,000 700 900 1,000 700 DEC 600 700 600 700 500 500 500 800 700 mean 1,464 836 873 945 982 823 1,282 1,118 845 mean 791 1,309 736 864 755 755 909 700 827 std dev 545 238 347 238 271 412 1,003 567 425 std dev 270 545 332 238 225 163 288 126 283 median 1,500 700 900 900 900 700 900 1,000 700 median 700 1,100 600 900 700 700 900 700 700 max 2,500 1,300 1,600 1,300 1,500 1,700 4,200 2,700 1,800 max 1,300 2,100 1,500 1,200 1,200 1,000 1,400 900 1,400 min 600 600 500 600 600 50 700 600 400 min 500 700 400 500 500 500 500 500 500 40 Table 2.13 Total Phosphorus (mg/l) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP IC NCF6 JAN 140 120 90 80 60 50 30 40 JAN 130 70 210 190 230 FEB 60 90 80 100 90 60 40 40 FEB 210 210 160 160 70 MAR 280 260 180 320 170 270 190 180 MAR 230 220 220 190 140 APR 100 120 130 50 40 40 40 80 APR 100 120 80 120 70 MAY 130 100 90 90 80 70 30 30 MAY 140 180 170 200 210 JUN 100 140 270 270 160 120 110 80 JUN 140 130 160 150 130 JUL 210 150 130 130 110 270 90 90 JUL 230 220 270 170 150 AUG 210 160 230 250 330 180 180 210 AUG 170 170 220 130 130 SEP SEP 210 260 160 170 250 OCT 190 230 200 250 160 90 90 70 OCT 150 150 200 220 390 NOV 130 120 140 180 80 80 100 80 NOV 80 110 90 80 100 DEC 100 80 90 70 80 60 70 110 DEC 140 110 90 80 80 mean 150 143 148 163 124 117 88 92 mean 161 163 169 155 155 std dev 62 54 61 91 77 81 53 54 std dev 48 55 57 43 90 median 130 120 130 130 90 80 90 80 median 145 160 165 165 135 max 280 260 270 320 330 270 190 210 max 230 260 270 220 220 min 60 80 80 50 40 40 30 30 min 80 70 80 80 80 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SR-WC 6RC LCO GCO SR BRN HAM JAN 390 70 40 70 140 90 100 160 70 JAN 50 70 10 140 50 100 40 50 70 FEB 200 60 40 80 90 60 40 60 70 FEB 30 40 10 90 30 110 30 30 80 MAR 90 60 40 120 100 80 50 70 50 MAR 50 70 10 50 10 150 10 40 80 APR 280 170 60 150 190 160 150 60 30 APR 110 70 60 240 120 370 100 130 170 MAY 120 170 170 210 230 130 210 140 140 MAY 120 110 60 210 90 530 80 110 260 JUN 400 180 160 250 240 150 220 240 190 JUN 150 140 40 160 90 390 90 100 140 JUL 270 300 310 220 240 10 1,390 120 70 JUL 170 240 80 170 160 600 120 80 360 AUG 240 200 220 190 380 110 460 200 120 AUG 180 160 80 180 90 290 100 100 160 SEP SEP OCT 380 350 380 250 370 170 430 430 360 OCT 160 230 80 180 90 280 90 100 180 NOV 140 40 60 110 160 60 160 70 80 NOV 80 180 10 110 10 210 10 60 110 DEC 120 390 140 170 220 110 150 100 210 DEC 80 140 60 90 50 20 20 70 20 mean 239 181 147 165 215 103 305 150 126 mean 107 132 45 147 72 277 63 79 148 std dev 110 116 111 62 91 47 367 105 92 std dev 50 64 29 55 44 175 39 30 92 median 240 170 140 170 220 110 160 120 80 median 110 140 60 160 90 280 80 80 140 max 400 390 380 250 380 170 1,390 430 360 max 180 240 80 240 160 600 120 130 360 min 90 40 40 70 90 10 40 60 30 min 30 40 10 50 10 20 10 30 20 41 0 20 40 60 80 100 120 140 160 180 NC11 AC DP IC NAV HB BRR M61 M54 M35 M23 M18 NCF117 NCF6 B210 To t a l P h o s p h o r u s ( mg/ L ) Figure 2.5 Total Phosphorus at the Lower Cape Fear River Program mainstem stations, 1995-2017 versus 2018. 1995-2017 2018 42 Table 2.14 Orthophosphate (mg/l) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP BBT IC NCF6 JAN 50 30 30 30 30 20 10 10 JAN 110 100 80 20 50 10 FEB 30 60 40 60 50 40 30 20 FEB 50 40 50 40 40 80 MAR 20 30 20 40 30 20 10 10 MAR 30 40 50 50 60 20 APR 40 40 30 30 20 20 10 10 APR 40 30 30 20 40 20 MAY 40 30 40 20 40 20 20 10 MAY 30 30 30 30 30 50 JUN 30 60 40 60 50 40 30 20 JUN 50 40 50 40 40 80 JUL 60 60 40 40 40 30 20 10 JUL 70 90 100 80 80 30 AUG 30 30 40 50 50 30 30 30 AUG 30 40 40 50 40 50 SEP SEP 70 110 60 50 50 50 OCT 70 70 70 70 60 50 40 30 OCT 30 30 20 70 40 90 NOV 30 30 30 20 20 20 20 10 NOV 20 30 30 30 30 30 DEC 20 30 20 30 30 20 20 10 DEC 30 40 30 20 20 30 mean 38 43 36 41 38 28 22 15 mean 47 52 48 42 43 45 std dev 16 16 14 17 13 11 10 8 std dev 26 30 23 19 16 26 median 30 30 40 40 40 20 20 10 median 35 40 45 40 40 40 max 70 70 70 70 60 50 40 30 max 110 110 100 80 80 90 min 20 30 20 20 20 20 10 10 min 20 30 20 20 20 10 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SR-WC 6RC LCO GCO SR BRN HAM JAN 240 10 5 10 20 10 20 70 10 JAN 20 30 5 50 10 50 5 10 10 FEB 240 40 20 20 70 50 40 60 60 FEB 50 70 10 40 20 150 5 30 40 MAR 120 10 10 20 20 10 10 20 5 MAR 10 20 20 20 5 50 5 10 20 APR 220 10 10 30 30 10 40 20 10 APR 30 50 5 30 20 170 5 10 30 MAY 130 30 30 30 20 10 40 40 50 MAY 40 70 10 40 20 230 10 20 40 JUN 240 40 20 20 70 50 40 60 60 JUN 50 70 10 40 20 150 5 30 40 JUL 130 30 10 20 5 20 480 40 20 JUL 50 90 20 60 30 300 5 10 40 AUG 120 50 40 40 20 20 150 60 10 AUG 60 40 10 60 20 140 5 10 50 SEP SEP OCT 230 80 60 60 120 40 180 60 60 OCT 70 170 40 70 30 130 10 10 50 NOV 70 10 10 20 20 10 50 20 20 NOV 20 70 10 30 20 80 10 10 20 DEC 130 20 20 30 70 30 80 20 50 DEC 20 50 10 30 10 20 5 20 20 mean 170 30 21 27 42 24 103 43 32 mean 38 66 14 43 19 134 6 15 33 std dev 64 22 16 13 35 16 136 20 23 std dev 19 40 10 16 8 83 2 8 13 median 130 30 20 20 20 20 40 40 20 median 40 70 10 40 20 140 5 10 40 max 240 80 60 60 120 50 480 70 60 max 70 170 40 70 30 300 10 30 50 min 70 10 5 10 5 10 10 20 5 min 10 20 5 20 5 20 5 10 10 43 Table 2.15 Chlorophyll a (mg/l) during 2018 at the Lower Cape Fear River Program stations. NAV HB BRR M61 M54 M35 M23 M18 NC11 AC DP BBT IC NCF6 JAN 2 1 1 1 1 1 2 2 JAN 0 1 0 1 1 0 FEB 2 1 3 2 2 2 7 5 FEB 5 6 3 3 2 1 MAR 3 2 3 2 4 5 10 13 MAR 6 3 4 4 3 1 APR 1 1 1 1 1 2 3 3 APR 2 2 2 1 2 1 MAY 2 3 2 1 2 5 6 5 MAY 2 1 2 1 1 1 JUN 1 1 4 1 4 6 4 5 JUN 1 1 1 1 1 0 JUL 3 7 21 11 4 9 11 12 JUL 2 2 3 2 2 0 AUG 1 1 1 1 1 1 1 1 AUG 1 1 1 1 1 0 SEP SEP 2 3 1 1 1 7 OCT 2 3 2 2 2 1 2 3 OCT 2 2 1 11 7 13 NOV 2 2 2 2 3 3 2 4 NOV 2 2 1 1 1 2 DEC 3 3 2 1 2 1 1 6 DEC 2 2 2 2 1 1 mean 2 2 4 2 2 3 4 5 mean 2 2 2 2 2 2 std dev 1 2 6 3 1 3 4 4 std dev 2 1 1 3 2 4 median 2 2 2 1 2 2 3 5 median 2 2 2 1 1 1 max 3 7 21 11 4 9 11 13 max 6 6 4 11 7 13 min 1 1 1 1 1 1 1 1 min 0 1 0 1 1 0 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SR-WC 6RC LCO GCO SR BRN HAM JAN 1 2 3 11 28 2 2 1 0 JAN 3 2 2 4 1 2 11 2 4 FEB 1 18 6 7 9 1 12 1 2 FEB 2 3 2 3 2 3 16 2 5 MAR 1 1 1 3 12 2 1 0 1 MAR 1 2 2 1 1 1 9 0 3 APR 4 2 3 5 11 5 3 1 12 APR 1 3 2 6 1 2 14 2 3 MAY 4 1 2 13 2 2 1 1 2 MAY 1 2 0 0 0 1 18 1 2 JUN 3 1 2 11 5 4 1 1 1 JUN 0 2 1 1 1 2 16 1 1 JUL 4 4 13 17 22 2 38 1 13 JUL 1 3 1 1 1 2 43 2 5 AUG 25 1 3 9 13 2 0 0 6 AUG 0 2 1 1 1 1 4 1 1 SEP SEP OCT 4 1 20 39 18 7 7 17 24 OCT 0 0 0 1 1 2 4 6 0 NOV 1 1 1 3 1 2 0 1 5 NOV 0 1 0 1 0 2 5 2 0 DEC 3 1 1 2 1 6 1 0 1 DEC 1 1 1 2 4 2 2 1 1 mean 5 3 5 11 11 3 6 2 6 mean 1 2 1 2 1 2 13 2 2 std dev 7 5 6 10 9 2 11 5 7 std dev 1 1 1 2 1 1 11 2 2 median 3 1 3 9 11 2 1 1 2 median 1 2 1 1 1 2 11 2 2 max 25 18 20 39 28 7 38 17 24 max 3 3 2 6 4 3 43 6 5 min 1 1 1 2 1 1 0 0 0 min 0 0 0 0 0 1 2 0 0 44 0 1 2 3 4 5 6 7 NC11 AC DP IC NAV HB BRR M61 M54 M35 M23 M18 NCF117 NCF6 B210 BBT Ch l o r o p h y l l a (mg/ L ) Figure 2.7 Chlorophyll a at the Lower Cape Fear River Program mainstem stations, 1995-2017 versus 2018. 1995-2017 2018 45 Table 2.16 Fecal Coliform (cfu/100 mL) and Enterococcus (MPN) during 2018 at the Lower Cape Fear River Program stations. ENTEROCOCCUS NC11 AC DP IC NCF6 NAV HB BRR M61 M54 M35 M23 M18 JAN 19 19 28 37 5 5 91 JAN 31 51 5 10 5 20 FEB 390 290 55 172 10 46 55 FEB 22 57 94 75 67 101 MAR 28 28 55 10 37 546 10 MAR 97 85 63 20 10 96 APR 46 5 28 28 55 46 46 APR 5 5 10 5 5 5 MAY 19 73 73 19 73 10 82 MAY 20 41 41 62 20 20 JUN 14 23 41 32 43 37 37 JUN 30 31 20 26 89 5 JUL 46 181 5 19 230 19 46 JUL 10 31 5 5 5 5 AUG 127 181 46 100 5 127 91 AUG 20 10 10 5 10 10 SEP 19 28 46 91 280 SEP OCT 55 37 82 145 819 28 10 OCT 10 20 20 5 5 5 NOV 55 55 46 5 109 91 28 NOV 5 10 5 5 10 5 DEC 320 300 109 91 37 5 5 DEC 20 20 85 31 31 5 mean 95 102 51 62 142 87 46 mean 25 33 33 23 23 25 std dev 121 103 26 54 221 149 30 std dev 24 23 32 24 27 35 max 390 300 109 172 819 546 91 max 97 85 94 75 89 101 min 14 5 5 5 5 5 5 min 5 5 5 5 5 5 Geomean 50 55 42 39 51 34 32 Geomean 17 25 19 13 13 12 ANC SAR GS NC403 PB LRC ROC NCF117 SC-CH B210 COL SRWC 6RC LCO GCO SR BRN HAM JAN 37 28 5 5 19 37 109 163 340 JAN 37 172 190 530 340 145 210 91 728 FEB 10 82 37 37 5 19 163 240 64 FEB 28 19 55 55 37 37 28 73 1,180 MAR 109 145 82 163 190 28 728 46 73 MAR 19 46 37 64 28 73 73 136 199 APR 530 210 46 163 73 136 340 28 154 APR 19 37 440 1,460 480 199 240 310 1,550 MAY 37 145 163 1,270 340 163 410 37 200 MAY 64 109 28 55 290 154 127 136 217 JUN 91 145 28 260 235 73 340 260 136 JUN 55 118 37 73 73 100 64 240 91 JUL 240 37 127 200 60,000 320 910 10 217 JUL 55 109 210 136 570 19 430 520 217 AUG 250 270 370 250 1,730 355 340 37 270 AUG 100 728 19 460 370 300 163 819 260 SEP SEP OCT 37 144 250 270 3000 480 172 1,180 480 OCT 28 10 100 200 91 127 172 270 1,000 NOV 28 73 19 82 24 37 240 46 145 NOV 82 82 136 91 73 230 118 320 430 DEC 19 28 5 5 5 5 64 10 5 DEC 37 28 28 19 19 28 37 37 55 mean 126 119 103 246 5,966 150 347 187 189 mean 48 133 116 286 216 128 151 268 539 std dev 151 74 112 337 17,111 155 248 326 129 std dev 25 194 121 405 191 85 110 219 479 max 37 270 370 1,270 60,000 480 910 1,180 480 max 100 728 440 1,460 570 300 430 819 1,550 min 10 28 5 5 5 5 64 10 5 min 19 10 19 19 19 19 28 37 55 Geomean 65 93 49 99 172 73 267 66 127 Geomean 41 67 72 133 123 95 114 191 340 46 0 10 20 30 40 50 60 70 80 90 100 NC11 AC DP IC NAV HB NCF117 NCF6 B210 BRR M61 M54 M35 M23 M18 Fe c a l C o l i f o r m B a c t e r i a ( C F U / 1 0 0 m L ) Figure 2.8 Geometric Mean Fecal Coliform (NC11-B210) and Enterococcus (BRR-M18) at the LCFRP mainstem stations, 1996-2017 (Entero 2012-2017 ) vs. 2018. 1996-2017 2018 47