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Progress Report_1_January_2008COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. NEW HANOVER COUNTY WATER QUALITY MONITORING PROGRAM FIRST PROGRESS REPORT Prepared by: Coastal Planning & Engineering of North Carolina, Inc. Marine Scientist: Brad Rosov, M.Sc. Prepared For: New Hanover County, North Carolina Recommended Citation: Rosov, B., 2008. New Hanover County Water Quality Monitoring Program: First Progress Report. New Hanover County, North Carolina: Coastal Planning & Engineering of North Carolina, Inc. 15p. January 2008   i COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. NEW HANOVER COUNTY WATER QUALITY MONITORING PROGRAM FIRST PROGRESS REPORT Table of Contents Introduction........................................................................................................................................1 Methods..............................................................................................................................................5 Physical Parameters........................................................................................................................5 Chemical and Biological Parameters..............................................................................................5 Results................................................................................................................................................5 Barnards Creek................................................................................................................................6 Futch Creek.....................................................................................................................................6 Lords Creek.....................................................................................................................................8 Motts Creek.....................................................................................................................................9 Pages Creek.....................................................................................................................................10 Prince Georges................................................................................................................................11 Smith Creek....................................................................................................................................12 Discussion..........................................................................................................................................13 Literature Cited..................................................................................................................................15 List of Figures Figure No. 1 Map of New Hanover County and watersheds included in this study..............................2 List of Tables   Table No. 1 List of Sampling Sites.......................................................................................................1 2 North Carolina Water Quality Standards..........................................................................4 3 Single sample standards for Enterococci as determined by the US EPA.........................4 4 Single sample standards for Enterococci as determined by the NC DENR Recreational Water Quality Program.....................................................................................................4 List of Appendices Appendix No. A Maps of the watersheds and sampling sites   1 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. INTRODUCTION The creeks in New Hanover County, North Carolina provide a wide range of recreational activities for thousands of local citizens and visiting tourists each year. Protection of the water quality within these creeks is a high priority of New Hanover County (County). As growth and development continue within the City of Wilmington and the County, water quality has been increasingly threatened due to many factors including aging infrastructure, increased impervious surface area and subsequent stormwater runoff. To address these issues, the County has administered a long-standing water quality monitoring program since 1993 designed to assess the water quality within the creeks located within the County. New Hanover County signed a contract with Coastal Planning & Engineering of North Carolina, Inc. in October 2007 to perform a water quality monitoring project within seven tidal creeks between the months of November 2007 and June 2008. The creeks included in this study are Pages and Futch Creek, which drain into the Intracoastal Waterway (ICW) and Lords, Motts, Barnards, Smith, and Prince Georges Creek, which drain into the Cape Fear River. As listed in Table 1 and displayed in Figure 1, a total of 22 sampling stations have been established for this project. Fifteen of the 22 sampling sites were previously monitored by the University of North Carolina at Wilmington. This historical data will be available for further analysis of trends within and between sites and will be incorporated into the Final Report. Table 1. List of Sampling Sites Creek Name Site Name Site Code Latitude Longitude Motts Creek Carolina Beach Road MOT-CBR 34° 08.610 77° 53.830 Motts Creek Normandy Drive MOT-ND 34° 08.373 77° 54.580 Motts Creek River Road MOT-RR 34° 07.752 77° 54.966 Lords Creek River Road LC-RR 34° 05.185 77° 55.275 Barnards Creek Carolina Beach Road BC-CBR 34° 09.522 77° 54.712 Barnards Creek River Road BC-RR 34° 09.525 77° 56.281 Smith Creek Castle Hayne Road SC-CH 34° 15.541 77° 56.325 Smith Creek 23rd Street SC-23 34° 15.472 77° 55.178 Smith Creek Candlewood Drive SC-CD 34° 17.438 77° 51.332 Smith Creek North Kerr SC-NK 34° 15.744 77° 53.256 Smith Creek Gordon Road SC-GR 34° 16.639 77° 52.037 Prince Georges Creek Marathon Landing PG-ML 34° 21.088 77° 55.349 Prince Georges Creek Castle Hayne Road PG-CH 34° 20.675 77° 54.217 Prince Georges Creek North College PG-NC 34° 20.331 77° 53.607 Futch Creek 4 FC-4 34° 18.068 77° 44.760 Futch Creek 6 FC-6 34° 18.178 77° 45.038 Futch Creek 8 FC-8 34° 18.25 77° 45.222 Futch Creek 13 FC-13 34° 18.214 77° 45.451 Futch Creek Foy Branch FC-FOY 34° 18.405 77° 45.358 Pages Creek Mouth PC-M 34° 16.209 77° 46.270 Pages Creek Bayshore Drive Down Stream PC-BDDS 34° 16.685 77° 47.673 Pages Creek Bayshore Drive Up Stream PC-BDUS 34° 16.623 77° 48.104   2 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. Figure 1: Map of New Hanover County and watersheds included in this study   3 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. The State of North Carolina has employed a series of classifications that apply to all waters in the State including streams, rivers, and lakes (NC Administrative Code, section 15A NCAC 2B .0200). These classifications are each meant to protect water bodies certain specified uses, such as aquatic life survival and reproduction, secondary recreation, primary recreation, shellfishing, and water supply. The classifications that apply to the creeks examined in this study are: C Sw: Freshwater that is protected for aquatic life and secondary recreation uses. The “Sw” supplemental classification indicates that these are swamp waters, and so are likely to have lower dissolved oxygen and pH than non-swamp streams due to natural conditions. However, a majority of the sites, including Lords Creek, Motts Creek, Barnards Creek, Smith Creek, and Prince Georges Creek, designated as C Sw by the State, are tidally influenced and have a brackish salinity range. SA: Saline waterbodies that are protected for shellfishing uses. This use requires a more stringent standard for fecal coliform. Protection for this use is in addition to protections for the uses specified by the “lower” classifications of SC and SB, which include aquatic life, secondary recreation, and primary recreation. This designation applies to Futch Creek and Pages Creek. Physical, chemical, and biological water quality monitoring data are currently being collected for this study. Physical parameters include temperature, salinity, conductivity, pH, turbidity, and dissolved oxygen. Chemical parameters monitored in this study include orthophosphate and nitrate/nitrite. Biological parameters include chlorophyll-a and two suites of fecal indicator bacteria: Enterococci and fecal coliform bacteria. Due to limited funding, fecal coliform samples were only collected from sampling sites located within Futch Creek and Pages Creek. Fecal coliform bacteria have been collected within these two watersheds in recent years. By continuing the collection of this bacteria indicator, a long term analysis of fecal coliform levels within these watersheds will be possible. Standards Water quality standards have been established legislatively for a number of these parameters (Table 2). Many of the water quality standards are described in the NC Administrative Code, section 15A NCAC 2H .0100. The water quality standards for Enterococci bacteria are described by the US EPA (US EPA, 1986) and in the NC Administrative Code, section 15A NCAC 18A .3402. The US EPA standards for Enterococci bacteria are based on incidents of gastrointestinal illness following contact with bathing waters. Single sample maximum allowable Enterococci density is 104 CFU/100ml, 158 CFU/100ml, 276 CFU/100ml, and 501 CFU/100ml for designated beach areas, swimming areas with moderate to full body contact, lightly used full body contact swimming areas, and infrequently used full body contact swimming areas, respectively (Table 3). When at least five samples are collected within a 30 day period, the US EPA recommends utilizing a geometric mean standard of 35 CFU/100ml. The North Carolina Recreational Water Quality Program (RWQ) adopted similar standards for Enterococci bacteria, also determined by the frequency of swimming activity. As defined by RWQ, Tier I swimming areas are used daily during the swimming season, Tier II swimming areas are used three days a week during the swimming season, and Tier III swimming areas are used on average 4 days a month during the swimming season. Single sample standards for Tiers   4 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. I, II, and III are 104 CFU/100ml, 276 CFU/100ml, and 500 CFU/100ml, respectively (Table 4). A geometric mean of 35 CFU/100ml within Tier I swimming areas may also be utilized if at least five samples are collected within 30 days. The creeks included in this study have not been designated within the RWQ tier system and therefore do not have Enterococci standards ascribed to them. Table 2. North Carolina Water Quality Standards Parameter Standard for SA Waters Standard for C Sw Waters Dissolved Oxygen 5.0 mg/L 4.0 mg/La Turbidity 25 NTU 50 NTU pH 6.8-8.5 6.0-9.0b Chlorophyll-a 40.0 mg/L 40.0 mg/L Fecal Coliform Geometric Mean (5 samples within 30 days) <14 CFU/100ml; single sample <43 CFU/100ml Geometric Mean (5 samples within 30 days) <200 CFU/100ml; single sample <400 CFU/100ml Enterococci Geometric Mean (5 samples within 30 days) <35 CFU/100mlc Geometric Mean (5 samples within 30 days) <35 CFU/100mlc a C Sw waters may have lower values caused by natural conditions b For swamp streams, pH may be as low as 4.3 if caused by natural conditions c Single sample standards to be determined by New Hanover County based on the tiered system employed by US EPA or NC DENR Recreational Water Quality Program   Table 3. Single sample standards for Enterococci as determined by the US EPA Single sample maximum Designated beach areas < 104 CFU/100 ml Swimming areas with moderate full body contact < 158 CFU/100 ml Lightly used full body contact swimming areas < 276 CFU/100 ml Infrequently used full body contact swimming areas < 501 CFU/100 ml Table 4. Single sample standards for Enterococci as determined by the NC DENR Recreational Water Quality Program Single sample maximum Tier I, swimming areas used daily during the swimming season <104 CFU/100 ml Tier II, swimming areas used three days a week during the swimming season <276 CFU/100 ml Tier III, swimming areas used on average four days a month during the swimming season <500 CFU/100 ml   5 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. METHODS The seven creeks included in this study were selected by County staff and individual sampling sites were selected by County staff in consultation with Coastal Planning & Engineering of North Carolina, Inc. These seven creeks are primarily located in the unincorporated portion of New Hanover County. Sampling sites were accessed from land, generally near a bridge or culvert crossing, or by boat. Each site was sampled one time per month during a high ebb tide. Tides were determined utilizing the National Oceanic and Atmospheric Administration’s (NOAA) Tides and Currents website (http://tidesandcurrents.noaa.gov/). Due to time constraints, monthly sampling events were conducted on three subsequent days each month. Lords Creek, Motts Creek, and Barnards Creek were visited on the first sampling day while Smith Creek and Prince Georges Creek were visited the second day. Futch Creek and Pages Creek were visited on the third day. Rainfall totals for the 24 hours prior to each sampling event were obtained from observations recorded at Wilmington International Airport as reported by NOAA’s National Weather Service web site (http://www.srh.noaa.gov/data/RAH/RTPRAH). Physical Parameters All physical measurements (temperature, salinity, conductivity, turbidity, dissolved oxygen, and pH) were taken in situ utilizing a 6820 YSI Multiparameter Water Quality Probe linked to a YSI 650 MDS display unit. The YSI Probe was calibrated each day prior to use. Physical measurements were taken from the surface at all sites (depth = 0.1m) and near the creek bottom at sites with depths greater than 0.5m. Following each sampling trip, the YSI Probe was post- calibrated to ensure physical parameters measured were within an acceptable range. Chemical and Biological Parameters Water samples were obtained for the laboratory analysis of chemical (nitrate/nitrite and orthophosphate) and biological (Enterococci, fecal coliform, and chlorophyll-a) parameters. These grab samples were collected in sterile bottles during a high ebb tide from the surface at each site (depth = 0.1m). Trained volunteers from Cape Fear Community College’s Marine Technology Department assisted Coastal Planning & Engineering of North Carolina, Inc. staff with the collection of water samples. Water samples were placed on ice immediately following collection and were delivered in coolers to Tritest Inc. of Wilmington, North Carolina for analysis. All analyses performed by Tritest, Inc. were conducted utilizing the following standard EPA approved methods: Orthophosphate: SM 4500E Nitrate/Nitrite: EPA 353.2 Chlorophyll-a: SM 10200H Fecal Coliform: SM 9222D Enterococcus: EnterolertE RESULTS The preliminary results described in this report represent data collected following the first two months of monitoring (November and December 2007). The results are organized by watershed and include data summaries displayed by parameter (physical, biological, and chemical).   6 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. Barnards Creek Both sampling sites within Barnards Creek watershed were monitored on 14 November and 12 December 2007. Generally, physical, chemical and biological parameters indicated good water quality within these sites. Dissolved oxygen at BC-CBR was relatively low in December, but within State standards for C Sw waters. Chlorophyll-a did not breach the 40ug/L standard; however the value of 24.6ug/L at BC-RR in November did approach this limit. Physical data collected from Barnards Creek Date Site Name Rainfall (inches) Depth (m) Temp. (oC) Specific Conductivity (us/cm) Salinity (ppt) Dissolved Oxygen (mg/L) Dissolved Oxygen (%) pH Turbidity (NTU) 11/14/07 BC-CBR 0.0 0.1 14.7 214 0.1 8.2 80 6.9 0 11/14/07 BC-CBR 0.0 1.0 13.6 228 0.1 6.0 58 6.9 8 11/14/07 BC-RR 0.0 0.1 16.2 26603 20.0 8.1 92 7.7 2 11/14/07 BC-RR 0.0 1.8 16.2 26661 20.1 8.1 93 7.7 2 12/12/07 BC-CBR 0.0 0.1 16.0 200 0.1 4.9 50 6.8 0 12/12/07 BC-CBR 0.0 1.0 14.2 197 0.1 5.1 52 6.7 4 12/12/07 BC-RR 0.0 0.1 14.8 29952 23.6 7.7 87 7.7 9 12/12/07 BC-RR 0.0 2.2 14.8 30167 23.8 7.7 88 7.7 12 Biological data collected from Barnards Creek Date Site Name Rainfall (inches) Previous Depth (m) Enterococci (CFU/100ml Chlorophyll-a (ug/L) 11/14/07 BC-CBR 0.0 0.1 88 1.0 11/14/07 BC-RR 0.0 0.1 38 24.6 12/12/07 BC-CBR 0.0 0.1 43 5.34 12/12/07 BC-RR 0.0 0.1 135 4.67 Chemical data collected from Barnards Creek Date Site Name Rainfall (inches) Previous Depth (m) Nitrate/Nitrite (mg/L) Orthophosphate (mg/L) 11/14/07 BC-CBR 0.0 0.1 0.12 0.01 11/14/07 BC-RR 0.0 0.1 0.36 0.05 12/12/07 BC-CBR 0.0 0.1 0.03 0.23 12/12/07 BC-RR 0.0 0.1 0.36 0.05 Futch Creek Five sampling sites within Futch Creek were monitored on 16 November and 14 December 2007. Fecal coliform counts were extremely high at all sites in November. It is important to note that a significant rainfall (0.4 inches) was measured within 24 hours of sampling in November. Enterococci counts were also higher at three of the five sampling sites in November   7 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. compared to December. Despite these high bacteria levels, chlorophyll-a, dissolved oxygen, turbidity, nitrate/nitrite, and orthophosphate values were well within their respective standards during this period at all sites. Physical data collected from Futch Creek Date Site Name Rainfall (inches) Depth (m) Temp. (oC) Specific Conductivity (us/cm) Salinity (ppt) Dissolved Oxygen (mg/L) Dissolved Oxygen (%) pH Turbidity (NTU) 11/16/07 FC-13 0.35 0.1 13.3 37644 31.9 8.3 97 8.0 0 11/16/07 FC-13 0.35 0.6 13.9 41705 34.8 8.3 98 8.1 4 11/16/07 FC-4 0.35 0.1 14.3 43467 36.2 8.0 98 8.2 0 11/16/07 FC-4 0.35 0.4 14.4 43640 36.2 8.3 101 8.2 0 11/16/07 FC-6 0.35 0.1 13.9 42932 35.6 8.1 98 8.1 0 11/16/07 FC-6 0.35 0.8 14.0 43048 36.0 8.2 100 8.1 0 11/16/07 FC-8 0.35 0.1 13.9 42690 35.8 8.1 97 8.1 0 11/16/07 FC-8 0.35 0.4 14.0 42782 35.8 8.2 98 8.1 0 11/16/07 FC-FOY 0.35 0.1 13.9 41919 35.1 8.3 100 8.1 0 11/16/07 FC-FOY 0.35 0.6 14.0 42592 35.6 8.5 102 8.1 0 12/14/07 FC-13 0.0 0.1 17.2 42912 33.1 6.5 82 8.0 1 12/14/07 FC-13 0.0 0.5 16.8 46333 36.5 6.5 83 8.1 5 12/14/07 FC-4 0.0 0.1 16.4 47880 38.2 6.8 88 8.1 0 12/14/07 FC-4 0.0 2.0 16.3 47885 38.2 7.3 94 8.1 1 12/14/07 FC-6 0.0 0.1 16.4 47880 38.2 6.8 88 8.1 0 12/14/07 FC-6 0.0 1.0 16.5 47735 37.9 7.5 97 8.1 0 12/14/07 FC-8 0.0 0.1 16.6 47072 37.3 6.8 86 8.1 0 12/14/07 FC-8 0.0 0.4 16.5 47184 37.4 6.9 89 8.1 0 12/14/07 FC-FOY 0.0 0.1 16.9 45357 35.5 6.6 85 8.1 0 12/14/07 FC-FOY 0.0 0.5 16.8 46009 36.1 7.0 90 8.1 1 Biological data collected from Futch Creek Date Site Name Rainfall (inches) Depth (m) Enterococci (CFU/100ml Fecal Coliform (CFU/100ml) Chlorophyll-a (ug/L) 11/16/07 FC-13 0.35 0.1 25 4400 1.0 11/16/07 FC-4 0.35 0.1 119 3800 1.0 11/16/07 FC-6 0.35 0.1 109 6400 2.1 11/16/07 FC-8 0.35 0.1 23 4800 4.3 11/16/07 FC-FOY 0.35 0.1 98 7000 2.1 12/14/07 FC-13 0.0 0.1 78 200 1.0 12/14/07 FC-4 0.0 0.1 70 50 1.0 12/14/07 FC-6 0.0 0.1 23 50 1.0 12/14/07 FC-8 0.0 0.1 34 100 1.0 12/14/07 FC-FOY 0.0 0.1 80 100 1.0   8 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. Chemical data collected from Futch Creek Date Site Name Rainfall (inches) Depth (m) Nitrate/Nitrite (mg/L) Orthophosphate (mg/L) 11/16/07 FC-13 0.35 0.1 0.05 0.01 11/16/07 FC-4 0.35 0.1 0.08 0.01 11/16/07 FC-6 0.35 0.1 0.04 0.01 11/16/07 FC-8 0.35 0.1 0.02 0.01 11/16/07 FC-FOY 0.35 0.1 0.04 0.01 12/14/07 FC-13 0.0 0.1 0.03 0.01 12/14/07 FC-4 0.0 0.1 0.02 0.01 12/14/07 FC-6 0.0 0.1 0.01 0.01 12/14/07 FC-8 0.0 0.1 0.01 0.01 12/14/07 FC-FOY 0.0 0.1 0.01 0.01 Lords Creek One sampling site was monitored within Lords Creek on 14 November and 12 December 2007. Dissolved oxygen was extremely low at both the surface and near the bottom during both sampling events. While turbidity did not exceed the State standard of 50 NTU, the observed values were higher than expected. Enterococci counts were also elevated during both months. Physical data collected from Lords Creek Date Site Name Rainfall (inches) Depth (m) Temp. (oC) Specific Conductivity (us/cm) Salinity (ppt) Dissolved Oxygen (mg/L) Dissolved Oxygen (%) pH Turbidity (NTU) 11/14/07 LC-RR 0.0 0.1 16.5 30011 22.7 8.8 103 7.7 1 11/14/07 LC-RR 0.0 1.4 16.5 30054 22.7 8.8 103 7.7 2 12/12/07 LC-RR 0.0 0.1 16.0 34778 27.0 7.8 93 7.6 24 12/12/07 LC-RR 0.0 1.3 16.0 34849 27.1 7.7 92 7.6 32 Biological data collected from Lords Creek Date Site Name Rainfall (inches) Depth (m) Enterococci (CFU/100ml Chlorophyll-a (ug/L) 11/14/07 LC-RR 0.0 0.1 548 7.5 12/12/07 LC-RR 0.0 0.1 158 6.7 Chemical data collected from Lords Creek Date Site Name Rainfall (inches) Depth (m) Nitrate/Nitrite (mg/L) Orthophosphate (mg/L) 11/14/07 LC-RR 0.0 0.1 0.31 0.03 12/12/07 LC-RR 0.0 0.1 0.20 0.03   9 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. Motts Creek Three sampling sites within the Motts Creek watershed were included in this study. Water quality within these three sites was monitored on 14 November and 12 December 2007. Dissolved oxygen was poor within MOT-CBR and MOT-ND in December. High levels of Enterococci bacteria were recorded at MOT-ND during both months. Nutrient levels, turbidity, pH, and chlorophyll-a were acceptable during both sampling events. Physical data collected from Motts Creek Date Site Name Rainfall (inches) Depth (m) Temp (C) Specific Conductivity (us/cm) Salinity (ppt) Dissolved Oxygen (mg/L) Dissolved Oxygen (%) pH Turbidity (NTU) 11/14/07 MOT-CBR 0.0 0.1 18.5 522 0.3 4.9 52 7.0 16 11/14/07 MOT-ND 0.0 0.1 14.2 447 0.2 6.7 65 7.2 0 11/14/07 MOT-RR 0.0 0.1 16.1 26013 19.5 8.3 94 7.5 0 11/14/07 MOT-RR 0.0 1.2 16.1 26002 19.5 8.3 94 7.5 0 12/12/07 MOT-CBR 0.0 0.1 18.5 508 0.3 3.2 34 6.8 5 12/12/07 MOT-ND 0.0 0.1 16.3 413 0.2 3.2 33 7.0 4 12/12/07 MOT-RR 0.0 0.1 16.0 26137 19.7 6.7 76 7.3 0 12/12/07 MOT-RR 0.0 1.6 16.0 25916 19.5 6.7 76 7.3 2 Biological data collected from Motts Creek Date Site Name Rainfall (inches) Depth (m) Enterococci (CFU/100ml Chlorophyll-a (ug/L) 11/14/07 MOT-CBR 0.0 0.1 56 25.6 11/14/07 MOT-ND 0.0 0.1 1414 6.4 11/14/07 MOT-RR 0.0 0.1 23 1.0 12/12/07 MOT-CBR 0.0 0.1 10 3.3 12/12/07 MOT-ND 0.0 0.1 129 5.3 12/12/07 MOT-RR 0.0 0.1 68 4.0 Chemical data collected from Motts Creek Date Site Name Rainfall (inches) Depth (m) Nitrate/Nitrite (mg/L) Orthophosphate (mg/L) 11/14/07 MOT-CBR 0.0 0.1 0.13 0.01 11/14/07 MOT-ND 0.0 0.1 0.05 0.03 11/14/07 MOT-RR 0.0 0.1 0.29 0.01 12/12/07 MOT-CBR 0.0 0.1 0.04 0.02 12/12/07 MOT-ND 0.0 0.1 0.02 0.01 12/12/07 MOT-RR 0.0 0.1 0.18 0.03   10 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. Pages Creek Three sampling sites were visited in the Pages Creek watershed on 16 November and 14 December 2007. Dissolved oxygen values recorded from PC-BDUS and PC-BDDS in December were below the State standard of 5.0mg/L. The fecal coliform levels were extremely high in November following a significant rainfall. Enterococci levels were elevated in both November and December. Chlorophyll-a, turbidity, pH, and chemical parameters were within acceptable ranges. Physical data collected from Pages Creek Date Site Name Rainfall (inches) Depth (m) Temp. (oC) Specific Conductivity (us/cm) Salinity (ppt) Dissolved Oxygen (mg/L) Dissolved Oxygen (%) pH Turbidity (NTU) 11/16/07 PC-BDDS 0.35 0.1 12.6 40790 35.2 7.5 88 8.0 0 11/16/07 PC-BDUS 0.35 0.1 14.5 25139 19.6 6.3 70 7.4 2 11/16/07 PC-M 0.35 0.1 14.1 43188 36.2 7.7 93 8.0 0 11/16/07 PC-M 0.35 1.6 14.1 43301 36.2 7.8 95 8.1 0 12/14/07 PC-BDDS 0.0 0.1 17.5 44825 34.5 4.8 61 7.8 3 12/14/07 PC-BDUS 0.0 0.1 19.0 30794 21.9 4.5 56 7.3 4 12/14/07 PC-M 0.0 0.1 16.7 48166 38.2 7.1 92 8.1 1 12/14/07 PC-M 0.0 1.5 16.7 48146 38.2 7.1 92 8.1 3 Biological data collected from Pages Creek Date Site Name Rainfall (inches) Depth (m) Enterococci (CFU/100ml Fecal Coliform (CFU/100ml) Chlorophyll-a (ug/L) 11/16/07 PC-BDDS 0.35 0.1 548 4200 1.0 11/16/07 PC-BDUS 0.35 0.1 135 7200 1.0 11/16/07 PC-M 035 0.1 70 5600 3.2 12/14/07 PC-BDDS 0.0 0.1 727 700 1.0 12/14/07 PC-BDUS 0.0 0.1 517 500 1.0 12/14/07 PC-M 0.0 1.5 121 50 1.0 Chemical data collected from Pages Creek Date Site Name Rainfall (inches) Depth (m) Nitrate/Nitrite (mg/L) Orthophosphate (mg/L) 11/16/07 PC-BDDS 0.35 0.1 0.04 0.03 11/16/07 PC-BDUS 0.35 0.1 0.04 0.04 11/16/07 PC-M 035 0.1 0.04 0.01 12/14/07 PC-BDDS 0.0 0.1 0.03 0.04 12/14/07 PC-BDUS 0.0 0.1 0.01 0.08 12/14/07 PC-M 0.0 1.5 0.01 0.01   11 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. Prince Georges Three sampling sites were visited in the Prince Georges Creek watershed on 15 November and 13 December 2007. Dissolved oxygen values recorded from PG-NC were well below the State standard of 4.0mg/L in both November and December. Dissolved oxygen at PG-CH was just above the acceptable limit in November and below the State standard during December. Enterococci levels were higher in November following the rain event compared to the values observed in December. Chlorophyll-a, turbidity, and chemical parameters were within acceptable ranges. Physical data collected from Prince Georges Creek Date Site Name Rainfall (inches) Depth (m) Temp. (oC) Specific Conductivity (us/cm) Salinity (ppt) Dissolved Oxygen (mg/L) Dissolved Oxygen (%) pH Turbidity (NTU) 11/15/07 PG-CH 0.2 0.1 14.0 801 0.5 5.1 49 7.4 0 11/15/07 PG-CH 0.2 1.0 13.9 837 0.5 4.8 46 7.4 4 11/15/07 PG-ML 0.2 0.1 14.5 1934 1.3 6.6 65 6.7 0 11/15/07 PG-NC 0.2 0.1 11.6 138 0.1 1.2 11 6.4 4 11/15/07 PG-NC 0.2 1.3 9.9 133 0.1 0.4 3 6.4 5 12/13/07 PG-CH 0.0 0.1 15.3 757 0.5 4.2 42 7.4 0 12/13/07 PG-CH 0.0 1.2 15.0 742 0.5 3.5 35 7.4 4 12/13/07 PG-ML 0.0 0.1 15.7 844 0.5 6.2 63 6.8 0 12/13/07 PG-NC 0.0 0.1 14.2 162 0.1 3.3 31 6.7 0 12/13/07 PG-NC 0.0 3.2 8.8 147 0.1 0.6 5 6.5 0 Biological data collected from Prince Georges Creek Date Site Name Rainfall (inches) Depth (m) Enterococci (CFU/100ml Chlorophyll-a (ug/L) 11/15/07 PG-CH 0.2 0.1 281 3.2 11/15/07 PG-ML 0.2 0.1 378 1.0 11/15/07 PG-NC 0.2 0.1 8 9.6 12/13/07 PG-CH 0.0 0.1 135 4.0 12/13/07 PG-ML 0.0 0.1 57 5.3 12/13/07 PG-NC 0.0 0.1 4 2.7 Chemical data collected from Prince Georges Creek Date Site Name Rainfall (inches) Depth (m) Nitrate/Nitrite (mg/L) Orthophosphate (mg/L) 11/15/07 PG-CH 0.2 0.1 0.35 0.04 11/15/07 PG-ML 0.2 0.1 0.05 0.01 11/15/07 PG-NC 0.2 0.1 0.1 0.01 12/13/07 PG-CH 0.0 0.1 0.01 0.07 12/13/07 PG-ML 0.0 0.1 0.05 0.02 12/13/07 PG-NC 0.0 3.2 0.01 0.01   12 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. Smith Creek Five sampling sites were visited in the Smith Creek watershed on 15 November and 13 December 2007. Generally, dissolved oxygen was good at all sites each month with the exception of low levels observed at SC-GR in December. Enterococci levels were elevated during both months at all sites with the exception of SC-CH in December. Nitrite/nitrate and chlorophyll-a levels were relatively high at Smith Creek sites compared to other watersheds studied in this program, particularly in November. Physical data collected from Smith Creek Date Site Name Rainfall (inches) Depth (m) Temp. (C) Specific Conductivity (us/cm) Salinity (ppt) Dissolved Oxygen (mg/L) Dissolved Oxygen (%) pH Turbidity (NTU) 11/15/07 SC-23 0.2 0.1 15.8 15891 11.5 8.5 92 7.5 3 11/15/07 SC-23 0.2 2.4 15.8 16177 11.7 8.4 91 7.6 4 11/15/07 SC-CD 0.2 0.1 15.4 166 0.1 7.9 79 7.2 7 11/15/07 SC-CH 0.2 0.1 16.0 21501 15.9 7.0 78 7.3 4 11/15/07 SC-CH 0.2 1.8 16.0 21762 16.1 6.8 76 7.4 8 11/15/07 SC-GR 0.2 0.1 15.4 191 0.1 6.4 64 7.0 0 11/15/07 SC-NK 0.2 0.1 14.9 8517 6.0 7.7 80 7.0 0 11/15/07 SC-NK 0.2 2.8 14.8 8790 6.2 7.7 80 7.1 0 12/13/07 SC-23 0.0 0.1 15.7 18805 13.8 7.5 82 7.3 1 12/13/07 SC-23 0.0 2.3 15.2 18911 14.1 7.7 84 7.3 1 12/13/07 SC-CD 0.0 0.1 16.6 257 0.2 5.8 59 7.1 0 12/13/07 SC-CH 0.0 0.1 15.1 23625 18.1 7.1 79 7.3 4 12/13/07 SC-CH 0.0 1.8 14.4 24254 18.6 7.2 79 7.4 6 12/13/07 SC-GR 0.0 0.1 16.0 219 0.1 3.8 38 6.8 0 12/13/07 SC-GR 0.0 0.5 15.9 212 0.1 3.3 33 6.8 6 12/13/07 SC-NK 0.0 0.1 16.2 5712 3.8 6.2 64 6.8 0 12/13/07 SC-NK 0.0 2.8 16.2 5802 3.8 6.2 64 6.8 1 Biological data collected from Smith Creek Date Site Name Rainfall (inches) Depth (m) Enterococci (CFU/100ml Chlorophyll-a (ug/L) 11/15/07 SC-23 0.2 0.1 238 34.2 11/15/07 SC-CD 0.2 0.1 517 13.9 11/15/07 SC-CH 0.2 0.1 285 3.2 11/15/07 SC-GR 0.2 0.1 250 27.8 11/15/07 SC-NK 0.2 0.1 99 9.6 12/13/07 SC-23 0.0 0.1 1300 6.0 12/13/07 SC-CD 0.0 0.1 1210 1.0 12/13/07 SC-CH 0.0 0.1 11 1.0 12/13/07 SC-GR 0.0 0.1 139 2.0 12/13/07 SC-NK 0.0 0.1 238 1.0   13 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. Chemical data collected from Smith Creek Date Site Name Rainfall (inches) Depth (m) Nitrate/Nitrite (mg/L) Orthophosphate (mg/L) 11/15/07 SC-23 0.2 0.1 0.25 0.03 11/15/07 SC-CD 0.2 0.1 0.24 0.06 11/15/07 SC-CH 0.2 0.1 0.35 0.04 11/15/07 SC-GR 0.2 0.1 0.07 0.01 11/15/07 SC-NK 0.2 0.1 0.01 0.01 12/13/07 SC-23 0.0 0.1 0.29 0.05 12/13/07 SC-CD 0.0 0.1 0.03 0.01 12/13/07 SC-CH 0.0 0.1 0.41 0.05 12/13/07 SC-GR 0.0 0.1 0.04 0.01 12/13/07 SC-NK 0.0 0.1 0.05 0.01 DISCUSSION It is not feasible to fully characterize the water quality of the creeks within New Hanover County after only two months of monitoring, however the data presented in this report will provide the public and County staff information on the current state of the creeks. Water quality is an important issue in the region due to the fact that there are many economic and recreational opportunities that are supported by the aquatic resources in and around these waterways. One of the greatest threats to water quality in this area is stormwater runoff created by increased impervious surface coverage (Mallin et al., 2000). Polluted stormwater runoff can have many adverse effects on plants, fish, animals and people. Excess nutrients can cause algae blooms while bacteria and other pathogens can wash into swimming areas and create health hazards. New Hanover County has experienced rapid growth and development over the past several decades. In 1990, the population within the County was 120,284. By 2006, the population grew over 50% to 182,591 (U.S. Census Bureau, 2006). Along with this population increase and subsequent stormwater runoff, numerous septic tanks, aging wastewater infrastructure, and other factors potentially impact the water quality within the County’s creeks. With this in mind, it is important to monitor the water quality of these local systems to determine potential impacts to both human health and ecosystem function. Algal blooms were not present at any site during the November and December sampling events. While sometimes problematic in the summer months, algal blooms are less common in the fall and winter when water temperature decreases. High levels of nutrients along with other physical conditions may trigger an algal bloom. Algal blooms may be indicated by high chlorophyll-a, low dissolved oxygen, and elevated pH values. Nitrite/nitrate and orthophosphate levels observed in this study were relatively low ranging between 0.01-0.41mg/L and 0.01-0.23mg/L, respectively. Chlorophyll-a levels ranged between 1.0 and 34.2ug/L which was within the acceptable level of 40ug/L as determined by the State. The pH values were also found to be within acceptable ranges (6.4 to 8.2). By evaluating these factors together, it was apparent that no algal blooms were present during either sampling period. Dissolved oxygen levels at a number of sites were below the State standard of 5.0 ml/L in SA waters and 4.0 mg/L in C Sw waters. The low dissolved oxygen values observed in MOT-CBR,   14 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. MOT-ND, and SC-GR may have been attributable to recent brush clearing of the right-of-way adjacent to the creek banks. If any of this plant material entered the creek, dissolved oxygen would decrease in response to bacterial respiration as the vegetation decomposes. Another factor influencing the water quality within Motts Creek may be the recent rapid development within the watershed. Currently, land is being cleared for a new mixed-use development along portions of the creek. A comparison of historical monitoring data within Motts Creek to the data collected during this study will help assess potential impacts of construction activity on the water quality within this watershed. PG-NC also experienced low dissolved oxygen during both months of monitoring. This site is characterized by very low flow and low pH and is situated in a swamp- like setting with a shallow, broad channel. Low dissolved oxygen is not unexpected in swamp environments. Bacteriological parameters were extremely high in November, particularly within Smith Creek, Prince Georges Creek, Futch Creek and Pages Creek. Samples were obtained on 15 and 16 November following the first significant rainfall since 25 October, which may have influenced these high counts. Stormwater runoff into these watersheds may have carried an increased load of fecal coliform and Enterococci due to the drought conditions prior to the rain event on the 16th. Although fecal indicator bacteria are not pathogenic themselves, they correlate well with levels of illness in swimmers, in particularly Enterococci (US EPA 1986). The US EPA has determined that the Enterococci provide the best correlation between swimming-associated gastrointestinal illnesses (US EPA, 1984, US EPA, 1986). As a result of the Beaches Environmental Assessment and Coastal Health (BEACH) Act of 2000, State governments were mandated by the US EPA to adopt Enterococci bacteria as an indicator of human health risk in coastal recreational waters. Due to their ability to survive longer in salt water, Enterococci is considered to be more conservative indicator of pathogenic contaminants compared to fecal coliform (Hanes and Fragela, 1967; del Mar Lleò et al., 2005). Other research supports the functionality of utilizing either or both indicators to test for risks associated with contact with water (Harrington and Cahoon, 2007). Additional data collected through the course of this eight month study will help provide insight as to the state of the water quality within the creeks in New Hanover County. In order to fully assess the water quality, it will be important to continue monitoring through the summer months when recreational activity increases within the creeks and water quality often diminishes. Long- term monitoring can help distinguish seasonal variation in the measured parameters.   15 COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. LITERATURE CITED del Mar Lleò, M.; Bonato, B.; Benedetti, D.; and Canepari, P., 2005. Survival of enterococcal species in aquatic environments. FEMS Microbiology Ecology, 5 4:189-196. Hanes, N.B.; and Frangela, R., 1967. Effect of seawater concentration on survival of indicator bacteria. Journal of the Water Pollution Control Federation 39:97-104. Harrington R.N. and Cahoon, L.B., 2007. Fecal indicator bacteria in the water and sediments of local boat ramps. pp. 68-80, within Environmental Quality of Wilmington and New Hanover County Watersheds 2005-2006, CMS Report 07-01, UNCW Center for Marine Science Research. Mallin, M.A.; Williams, K.E.; Esham, C.E.; and Lowe, P.R., 2000. Effect of human development on bacteriological water quality in coastal watersheds. Ecological Applications 10:1047-1056. U.S. Census Bureau, 2006 Population Estimates, Census 2000, 1990 Census U.S. Environmental Protection Agency. 1984. Health effects criteria for fresh recreational waters. EPA-600/1-84-004, U.S. Environmental Protection Agency, Washington, D.C. U.S. Environmental Protection Agency. 1986. Ambient Water Quality Criteria for Bacteria- 1986. EPA-440/5/84-002, U.S. Environmental Protection Agency, Washington, D.C   COASTAL PLANNING & ENGINEERING OF NORTH CAROLINA, INC. APPENDIX A Maps of Watersheds and Sampling Sites