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2021- 2022 Final ReportCPE-NC NEW HANOVER COUNTY WATER QUALITY MONITORING PROGRAM 2021-2022 FINAL REPORT Prepared by: Coastal Protection Engineering of North Carolina, Inc. Marine Scientist: Brad Rosov, M.Sc. Prepared For: New Hanover County, North Carolina Recommended Citation: Rosov, B., 2022. New Hanover County Water Quality Monitoring Program: 2021- 2022 Final Report. New Hanover County, North Carolina: Coastal Protection Engineering of North Carolina, Inc. September 2022 CPE-NC i EXECUTIVE SUMMARY This report represents the findings of the New Hanover County Water Quality Monitoring Program for the period July 2021 to June 2022. The results and long-term trends presented in this report are described from a watershed perspective. Since 2007 the county has partnered with Coastal Protection Engineering of North Carolina to test water quality at nineteen (20) monitoring stations within eight (8) tidal creeks in New Hanover County. These monitored include Barnards, Futch, Lords, Mott, Pages, Prince George, Smith, and Island. This monitoring period (2021-2022) included the addition of one (1) station at Island Creek to capture baseline data since future development is expected within the watershed. Each creek is monitored monthly for physical, chemical, and biological parameters that indicate overall water quality. The objective is to evaluate the current parameters at each creek to determine the impact (if any) of the built environment on water quality. In addition to the raw sampling results, a general assessment of the water quality is provided for each watershed. The general assessment gives each parameter the rating of either “Good”, “Fair”, or “Poor” depending on the percentage of samples that went above the State standard for turbidity, chlorophyll-a, and Enterococci, or below the State standard for dissolved oxygen. If the recorded value of a parameter went outside the acceptable range of the State standard less than 10% of the times sampled the watershed will receive a “Good” rating, a “Fair” rating 11%-25% of the times sampled, or a “Poor” rating for greater than 25% of the sampling times. Ratings by watershed during the 2021-2022 reporting period Parameter Barnards Creek Futch Creek Island Creek Lords Creek Mott Creek Pages Creek Prince George Creek Smith Creek Turbidity GOOD GOOD GOOD GOOD GOOD GOOD GOOD GOOD Dissolved Oxygen FAIR GOOD FAIR GOOD GOOD FAIR POOR GOOD Chlorophyll-a GOOD GOOD GOOD GOOD GOOD GOOD GOOD GOOD Enterococci GOOD GOOD GOOD GOOD GOOD POOR FAIR GOOD Long Term Trends Dissolved oxygen, turbidity, and chlorophyll-a levels fluctuate on a seasonal basis where, typically, levels decrease in the winter and increase in the summer. In general, turbidity and chlorophyll-a levels over time have not been of concern. Likewise, dissolved oxygen levels have not changed drastically from year to year and there have been minimal issues with low dissolved oxygen levels except for Prince George Creek where levels in the creek have been consistently low over time. This is due to its naturally slow water flow, which is more characteristic of swamp-like waters. Slower moving waters typically have lower dissolved oxygen levels. Overall, Enterococci bacteria levels during the 2021-2022 sampling for the majority of the watersheds remained the same with the exception Mott Creek which saw an improvement. There is a history of elevated levels of Enterococci bacteria that have been of carefully monitored over the years within Mott, Pages, Barnards, Smith, and Prince George creeks. Lords Creek and Futch Creek, on average, have contained relatively lower bacteria levels compared to the other creeks. CPE-NC ii It should be noted that monitoring at Island Creek began this year, hence, long-term trends are not available and have not been included in this report. In addition to monitoring the tidal creeks, in 2015 New Hanover County began monthly testing at the lake at Airlie Gardens due to concerns of water quality and noticeable algal blooms that had occurred over the years. The lake drains directly into Bradley Creek close to the Intracoastal Waterway. There are three sampling sites, one where contributing water enters the lake (intake), one in the middle of the lake, and one at the outfall before the water enters Bradley Creek. Since 2015 water quality monitoring has shown that dissolved oxygen varies significantly over an annual basis, increasing during the warmer summer months and decreasing during the colder winter months. Overall, there are no current concerns with the dissolved oxygen levels. Over past 7 years, the levels of the nutrient Nitrite/Nitrate have generally been higher at the intake compared to the sampling sites located at the middle and at the outfall of the lake. This trend with orthophosphate was observed during the first three years of monitoring, however the trend reversed over the past two years. High concentrations of orthophosphate and Nitrite/Nitrate have been linked to algae growth leading to algal blooms which can cause low dissolved oxygen and a decline in overall water quality. Since monitoring began, it has been observed that levels of orthophosphate have incrementally increased each year. Nitrate/Nitrite levels have also increased over time, however not in the same linear fashion as orthophosphate. Chlorophyll-a levels are also indicative of the presence of algal blooms. In recent years levels have increased at the middle and outfall of the lake compared to the sampling location in proximity to the intake into the lake. In general, Chlorophyll-a levels have steadily increased within the lake over time since sampling began in 2015. Improvement Efforts While urbanization and development are often factors that can impact water quality and while there is continued growth in New Hanover County, the ratings for many water quality parameters as depicted in this report have improved or remained steady over the past several years. This suggests that even though the unincorporated portions of New Hanover County continue to build out, there are factors minimizing the impact to water quality. While these factors facilitating this trend have not been identified, several notable efforts maybe contributing to these improvements. In addition to continued monitoring, New Hanover County has made efforts over the years to improve water quality including property acquisition using grant and trust fund sources, working with the Cape Fear Public Utility Authority to test sewer infrastructure, the installation of stormwater Best Management Practices (BMPs) such as raingardens, infiltration basins, and impervious surface retrofits, and bacteria source tracking. In 2008 New Hanover County partnered with University of North Carolina Wilmington’s Center for Marine Science and Coastal Protection Engineering of North Carolina, Inc. to conduct DNA source tracking which led to the identification of a human source for the bacteria in Pages Creek. This suggested that the source of the bacteria in the creek was from nearby wastewater infrastructure such as septic tanks systems and/or the municipal sewer system. Following the report, NHC Planning & Land Use partnered with the Cape Fear Public Utility Authority and the NHC Health Department to search for leaking septic or sewer, however, that investigation did not find the source of contamination. In 2013 the County partnered with UNC-Chapel Hill to expand the source tracking and conducted a study on the enterococci CPE-NC iii bacteria in Mott and Smith Creek and again at Pages Creek. That study found the presence of a human signature in the bacteria for all three creeks. In an attempt to identify any possible sources of bacteria entering Pages Creek, in 2019 New Hanover County partnered with the University of North Carolina Wilmington’s (UNCW) Socio-Environmental Analysis Laboratory and Coastal Protection Engineering (CPE) of North Carolina to conduct a thermal imagery scan of two portions of the creek adjacent to monitoring sites that have consistently detected elevated levels of Enterococci bacteria. Following the flight, UNCW and CPE analyzed the imagery and two areas that showed uncommon differences in temperature variation were identified. Ground truthing these areas revealed several subterranean groundwater seeps entering the creek from the streambank in the vicinity of sewer lift stations. In June and July of 2022 planning staff, CPE, and the Cape Fear Public Utility Authority coordinated to perform additional testing of the seep water coming from the creek bank in these two locations. Laboratory analysis of those samples detected human fecal pollution from both samples collected at one of the sampling locations while no human fecal pollution was detected at the other. The results from the additional testing and indicator analysis can be found in Appendix D of this report. At Airlie Gardens, to help combat problems associated with eutrophication and overall water quality the Park and Gardens department has implemented initiatives identified in their stormwater master plan. These initiatives include installing several aerators in the lake to increase the dissolved oxygen levels, restore a wetland area near the entry point where water enters the lake and the completion of a dredging operation effectively removing approximately 4,000 cubic yards of bottom sediment and material. The county continues to monitor the lake as it may take several years for a data trend to emerge. Data from this past year has not shown a reduction in nutrient loading into the lake. CPE-NC TABLE OF CONTENTS EXECUTIVE SUMMARY ....................................................................................................................... I LONG TERM TRENDS.............................................................................................................................I IMPROVEMENT EFFORTS ....................................................................................................................... II INTRODUCTION..................................................................................................................................1 CREEK SUMMARIES ............................................................................................................................1 BARNARDS CREEK ...............................................................................................................................1 FUTCH CREEK.....................................................................................................................................4 ISLAND CREEK ....................................................................................................................................5 LORDS CREEK.....................................................................................................................................6 MOTT CREEK .....................................................................................................................................7 PAGES CREEK .....................................................................................................................................8 PRINCE GEORGE CREEK ........................................................................................................................9 SMITH CREEK .....................................................................................................................................9 AIRLIE GARDENS .................................................................................................................................4 DISCUSSION ..................................................................................................................................... 12 PARAMETERS ................................................................................................................................... 13 AIRLIE GARDENS DISCUSSION ............................................................................................................... 16 APPENDIX A: ADDITIONAL CREEK DATA ............................................................................................ 17 BARNARDS CREEK ............................................................................................................................. 17 FUTCH CREEK................................................................................................................................... 20 ISLAND CREEK .................................................................................................................................. 25 LORDS CREEK................................................................................................................................... 27 MOTT CREEK ................................................................................................................................... 29 PAGES CREEK ................................................................................................................................... 32 PRINCE GEORGE ............................................................................................................................... 36 SMITH CREEK ................................................................................................................................... 40 AIRLIE GARDENS ............................................................................................................................... 45 APPENDIX B: LONG TERM TRENDS .................................................................................................... 50 DISSOLVED OXYGEN .......................................................................................................................... 50 TURBIDITY....................................................................................................................................... 51 CHLOROPHYLL-A ............................................................................................................................... 51 ENTEROCOCCI .................................................................................................................................. 52 APPENDIX C ..................................................................................................................................... 54 WATER CLASSIFICATIONS .................................................................................................................... 54 PARAMETERS ................................................................................................................................... 54 STANDARDS..................................................................................................................................... 57 METHODS....................................................................................................................................... 60 APPENDIX D: PAGES CREEK PCR ANALYSIS ........................................................................................ 61 LITERATURE CITED ........................................................................................................................... 62 CPE-NC INTRODUCTION New Hanover County’s (NHC) location is unique since with the exception of the northeastern boundary, it is surrounded by water on three sides; on the north and south by the Northeast Cape Fear River, on the west by the Cape Fear River, and on the east by the Atlantic Ocean. It is primarily a coastal county containing many creeks, streams, and water bodies that provide the opportunities for a wide range of recreational activities for thousands of local citizens and visiting tourists yearly. Due to its proximity to the Atlantic Ocean and the Intracoastal Waterway, NHC’s tidal creeks are not only used for recreation but are an important resource for the natural environment as they provide habitats for various plant and animal species. Tidal creeks are rich areas in terms of aquatic, terrestrial, and avian wildlife and can support complex food webs (Odum et al., 1984; Kwak and Zedle, 1997). Therefore, protection of the water quality within these creeks is a high priority for the County. Water quality has been monitored in New Hanover County since the early 1970s by the State in efforts to study the impacts of adjacent septic systems on water quality in tidal creeks. An increase in the closure of tidal creeks for shellfishing became an early concern for the citizens of New Hanover County and was a topic included in early land use plans. The ongoing water quality conversation within the community led to several watershed plans and in 1993, New Hanover County and the City of Wilmington partnered with the University of North Carolina Wilmington to conduct a long-standing water quality monitoring program. However, in November 2007, Coastal Protection Engineering of North Carolina, Inc. (CPE) began a separate, monthly water quality monitoring program on behalf of New Hanover County for the tidal creeks within the unincorporated parts of the County. The information presented in this report focuses on the results of this monitoring from July 2021 to June 2022. The creeks included in this study are Pages and Futch, which drain into the Atlantic Intracoastal Waterway (ICW), Island Creek, which drains into the Northeast Cape Fear River and Lords, Mott, Barnards, Smith, and Prince George, which drain into the Cape Fear River (Figure 1). In addition to the continued sampling from the seven tidal creeks, three sampling sites from within Airlie Gardens were added to the program during the 2015-2016 sampling efforts. The results described in this report represent the physical, biological, and chemical data collected monthly from all sampling sites from July 2021 through June 2022. These results are organized by watershed alphabetically, with the results of the eight (8) tidal creeks presented first, followed by the results from Airlie Gardens. Additional creek data, including parameters not summarized in this section, from the tidal creeks sampling sites and the Airlie Garden sampling sites are found in Appendix A. Based on the raw data, a quantitative system assigns a rating of “Good”, “Fair”, or “Poor” to a sampling station depending on the percentage of samples that went above the State standard for turbidity, chlorophyll-a, Enterococci, or below the State standard for dissolved oxygen. If the recorded value of a parameter went outside the acceptable range of the State standard less than 10% of the times sampled the station will receive a “Good” rating, a “Fair” rating 11%-25% of the times sampled, or a “Poor” rating for greater than 25% of the sampling times. This general description is useful when looking at trends from year to year and across the entire time frame of the program. Ratings for all parameters can be found in the Discussion section below. CPE-NC 2 Figure 1: Watersheds Monitored CPE-NC 3 CREEK SUMMARIES Barnards Creek • Location: South central New Hanover County and a portion in the City of Wilmington. (Monkey Junction, Echo Farms, Carriage Hills). • 1 Sampling Location: BC-CBR • Size: 4,234 Acres • Drains To: Cape Fear River • Land Use: Low and medium density residential, commercial, and retail uses along Carolina Beach Road, S. 17th Street, and S. College Road Year at a Glance • 2 (two) occurrences of dissolved oxygen below State standard • 0 (zero) occurrences above State standard for chlorophyll-a • 0 (zero) occurrences above State standard for turbidity • 0 (zero) occurrences above State standards for Enterococci Overall Assessment Overall, there were no issues with Barnards Creek for the year. However, dissolved oxygen did drop below the State standard twice. Parameter BC-CBR Turbidity (NTU) Good Dissolved Oxygen Fair Chlorophyll-a Good Enterococci Good CPE-NC 11 Futch Creek • Location: Northeast New Hanover County and a portion of Pender County (Porters Neck, Scotts Hill). • 4 Sampling Locations: FC-4, FC-6, FC-13, FC-FOY • Size: 3,429 Acres • Drains To: Intracoastal Waterway • Land Use: Low density residential and some commercial/retail uses along U.S. 17. Year at a Glance • 2 (two) occurrences of dissolved oxygen below State standard • 0 (zero) occurrences above State standard for chlorophyll-a • 0 (zero) occurrences above State standard for turbidity • 0 (zero) occurrences above State standards for Enterococci Overall Assessment Overall, there were no issues with Futch Creek for the year. Low dissolved oxygen was observed within the upper reaches of the creek at FC-13 on two occasions. Parameter FC-4, FC-6, FC-13, FC-FOY Turbidity (NTU) Good Dissolved Oxygen Good, Good, Fair, Good Chlorophyll-a Good Enterococci Good CPE-NC 5 Island Creek • Location: Northeast New Hanover County and portions of Pender County (Sidbury Road & Holly Shelter Road). • 1 Sampling Location: IC-HS • Size: 12,919 Acres • Drains To: NE Cape Fear River • Land Use: Undeveloped, low density residential. Year at a Glance • 3 (three) occurrences of dissolved oxygen below State standard • 0 (zero) occurrences above State standard for chlorophyll-a • 0 (zero occurrences above State standard for turbidity • 0 (zero) occurrences above State standards for Enterococci Overall Assessment During the first year of water quality monitoring within Island Creek, dissolved oxygen was determined to be fair while all other parameters were good. Parameter IC-HS Turbidity (NTU) Good Dissolved Oxygen Fair Chlorophyll-a Good Enterococci Good CPE-NC 6 Lords Creek • Location: Southwest New Hanover County (Veterans Park, River Road). • 1 Sampling Location: LC-RR • Size: 1,076 Acres • Drains To: Cape Fear River • Land Use: Low density residential and commercial/retail uses along Carolina Beach Road. Year at a Glance • 0 (zero) occurrences of dissolved oxygen below State standard • 0 (zero) occurrences above State standard for chlorophyll-a • 0 (zero occurrences above State standard for turbidity • 0 (zero) occurrences above State standards for Enterococci Overall Assessment Overall, there were no issues with Lords Creek for the year as no parameters exceeded their respective standards. Parameter LC-RR Turbidity (NTU) Good Dissolved Oxygen Good Chlorophyll-a Good Enterococci Good CPE-NC 7 3 Mott Creek • Location: South central New Hanover County (Monkey Junction, Silver Lake, Piner Road). • 2 Sampling Locations: MOT-ND, MOT-CBR • Size: 2,906 Acres • Drains To: Cape Fear River • Land Use: Low to Moderate density residential with commercial and retail along Carolina Beach Road and S. College Road. Year at a Glance • 1 (one) occurrence of dissolved oxygen below State standard • 1 (one) occurrence above State standard for chlorophyll-a • 0 (zero) occurrence above State standard for turbidity • 2 (two) occurrences above State standards for Enterococci Overall Assessment Overall, the water quality within Mott Creek for the year was good. While Enterococci levels have decreased over the years, there were two exceedances at the Normandy Drive site (MOT-ND) this year. Parameter MOT-CBR, MOT-ND Turbidity (NTU) Good Dissolved Oxygen Good Chlorophyll-a Good Enterococci Good, Fair CPE-NC 8 Pages Creek • Location: Northeastern New Hanover County. (Middle Sound, Ogden, Porters Neck). • 3 Sampling Locations: PC-BDUS, PC-BDDS, PC-M • Size: 4,124 Acres • Drains To: Intracoastal Waterway • Land Use: Low density residential and some commercial/retail uses along U.S. 17. Year at a Glance • 4 (four) occurrences of dissolved oxygen below State standard. • 1 (one) occurrence above State standard for chlorophyll-a • 0 (zero occurrences above State standard for turbidity • 11 (eleven) occurrences above State standards for Enterococci Overall Assessment Dissolved Oxygen levels were below state standards a few times throughout the year at all sites while Enterococci levels remained elevated at the upstream and downstream sites. Parameter PC-BDDS, PC-BDUS, PC-M Turbidity (NTU) Good Dissolved Oxygen Poor, Good, Good Chlorophyll-a Good Enterococci Poor, Poor, Good CPE-NC 9 Prince George Creek • Location: North New Hanover County (Castle Hayne). • 4 Sampling Locations: PG-CH, PG-ML, PG-NC • Size: 10,875 Acres • Drains To: Northeast Cape Fear River • Land Use: Low density residential, agricultural, and some commercial/retail uses Castle Hayne Road and N. College Road. Year at a Glance • 14 (fourteen) occurrences of dissolved oxygen below State standard • 0 (zero) occurrences above State standard for chlorophyll-a • 0 (zero) occurrences above State standard for turbidity • 4 (four) occurrences above State standards for Enterococci Overall Assessment Dissolved Oxygen at PG-NC was below state standards throughout the year with the other sites also experiencing issues with DO. There were 4 exceedances of state standards for Enterococci, 3 of which occurred in September. Parameter PG-CH, PG-ML, PG-NC Turbidity (NTU) Good Dissolved Oxygen Fair, Fair, Poor Chlorophyll-a Good Enterococci Fair, Good, Good CPE-NC 10 Smith Creek • Location: Central New Hanover County including portions of City of Wilmington (Wrightsboro, ILM, Kings Grant, Coastal Carolina). • 5 Sampling Locations: SC-CH, SC-23, SC-NK, SC-GR, SC-CD • Size: 17,535 Acres • Drains To: Cape Fear River • Land Use: Moderate density residential within the city, light industrial around the airport, some agricultural along Kerr Avenue, and some commercial/retail uses along U.S. 17. Year at a Glance • 2 (two) occurrences of dissolved oxygen below State standard • 1 (one) occurrence above State standard for chlorophyll-a • 0 (zero) occurrences above State standard for turbidity • 3 (three) occurrences above State standards for Enterococci Overall Assessment Overall, there were no concerns with Smith Creek for the year. However, Enterococci bacteria did exceed state standards three (3) times across 5 sites. Parameter SC-CH, SC-23, SC-NK, SC-GR, SC-CD Turbidity (NTU) Good Dissolved Oxygen Good Chlorophyll-a Good Enterococci Good, Good, Good, Fair, Good CPE-NC 11 Airlie Gardens • Location: City of Wilmington. • 4 Sampling Locations: AC-IN, AC-AD, AC- OUT • Size: 10 Acre Freshwater Lake • Drains To: Bradley Creek • Land Use: On a conservation site surrounded by low density residential. Year at a Glance • 0 (zero) occurrences of dissolved oxygen below State standard • 13 (thirteen) occurrences above State standard for chlorophyll-a • 1 (one) occurrence above State standard for turbidity *Enterococci is not measured at Airlie Gardens Overall Assessment There are no issues with dissolved oxygen or turbidity. However, Chlorophyll-a, Nitrate/Nitrite, and Orthophosphate levels have increased over the years. It may take several years for a trend to emerge after the completion of recent water quality improvement projects, and it is recommended to continue monitoring. CPE-NC 12 DISCUSSION Water quality is an important issue in the region since there are many economic and recreational opportunities that are supported by the aquatic resources in and around these waterways. In New Hanover County, different factors can affect water quality with a major one being land use. In more rural parts of the county, agriculture and farming can introduce increased amounts of chemicals like those found in fertilizer, as well and bacteria from animal waste. Additionally, failing or poorly maintained septic systems can increase bacteria in a watershed. In more urbanized areas, experts have identified stormwater runoff created by increased impervious surface coverage (Mallin et al., 2000) as a reason for increases in chemicals like those found in fertilizer for landscaped lawns, as well as bacteria from pet waste. Like rural areas, urban areas can also see human bacteria introduced into nearby waterways if there are deficiencies or leaks in the sanitary sewer system. Due to many of the contaminants found in stormwater runoff and its ability to concentrate especially after rain events, adverse effects can be imposed upon plants, fish, animals, and people. Excess nutrients can cause algal blooms while bacteria and other pathogens can wash into swimming areas and create health hazards. New Hanover County has experienced rapid growth and development over the past several decades. In 1990, the population within the County was 120,284. By 2006, the population grew over 50% to 182,591 (U.S. Census Bureau, 2006). Furthermore, the County’s population as of July 2014 was estimated to be 216,995 and has grown by 4% to 225,702 based on the 2020 census. While long term monitoring suggests that development and continued growth in New Hanover County may be altering water quality within its tidal creeks, a more recent assessment of the ratings for many water quality parameters as depicted in this report have improved or remained steady over the past several years. This suggests that even though the unincorporated portions of New Hanover County continue to build out, there are factors minimizing the impact to water quality. One rational may be the inclusion of state stormwater controls required for all new development which aims to mitigate stormwater on site. Additionally, the county has created a new stormwater services program to help maintain and improve drainage, primarily in areas developed prior state stormwater regulations. However, these and other factors have not been clearly identified as factors that may be facilitating this trend at this point but there have been several notable efforts made that maybe contributing to the improved water quality. From a bacteria perspective, in 2017, the Cape Fear Public Utility Authority (CFPUA) completed work to provide the Marquis Hills subdivision within the Mott Creek watershed with sewer service providing a more reliable way to treat sewage. Additionally, the CFPUA through their capital improvement plan, identifies and prioritizes projects to upgrade aging infrastructure like pumpstations, and programs like “Find it and Fix it” to maintain the integrity of the sewer system. For overall water quality, the county, continues to work toward preventing further deterioration and loss of public uses in surface water through initiatives such as the implementation of best management practices (BMPs) and promoting low impact development. In addition, the New Hanover County Stormwater Services Program continues to work on and has completed numerous drainage improvement projects. With this in mind, it is important to continue to monitor the water quality and assess the potential impacts to both human health and ecosystem function as conditions change. The long-term water quality monitoring results suggest that the seven (7) creeks that have experienced good water quality in terms of turbidity and chlorophyll-a levels over the course of the fourteen (14) year study thus far. The one parameter, however, that has been problematic has been Enterococci bacteria. Of the 3,189 samples collected and analyzed since June 2008, 722 samples (23% of all samples) have CPE-NC 13 exceeded the State standard for this bacterium. This analysis does not include data from Island Creek which was added to the monitoring program this past year and therefore was not included in the long- term data set. Parameters Physical and biological water quality monitoring data have been collected at each of the tidal creek sampling locations. Physical parameters include temperature, salinity, conductivity, pH, turbidity, and dissolved oxygen. Chemical parameters monitored in this study include orthophosphate and nitrate/nitrite. Biological parameters include chlorophyll-a and Enterococci, a fecal indicator for bacteria. At the Airlie Gardens sampling locations, the same physical parameters were collected in addition to chemical parameters including orthophosphate and nitrate/nitrite. It was determined at that time that bacterial contamination was non problematic, Enterococci samples are not collected at Airlie Gardens. Physical Parameter Discussion Over the past fourteen (14) years of water quality monitoring, some trends have emerged. Typically, water quality degrades as the water temperature increases and oxygen is not as readily dissolved in the water column. This phenomenon has been observed while investigating the long-term trends of water quality in this study. The dissolved oxygen along with chlorophyll-a and turbidity levels generally increased during the warmer summer months. The longer summer days allow for increased photosynthetic activity that, as a result, allows for an increase in phytoplankton blooms. While often problematic in the summer months, algal blooms are less common in the fall and winter when water temperature decreases. High levels of chlorophyll-a and nutrients along with increases in pH and turbidity may indicate the presence of an algal bloom. Throughout the course of this study, pH values and turbidity measurements were generally found to be within acceptable ranges while only three (3) chlorophyll-a samples exceeded the State standard during the 2021-2022 study period. Overall Watershed Rating In general, the dissolved oxygen within Barnards Creek, Lords Creek, Mott Creek, and Smith Creek has been rated “Good” through the course of this long-term study with few exceptions. Barnards Creek had declined in term of dissolved oxygen in recent years, however it had improved to “Good” again over the four (4) of the past five (5) years with it reverting back to “Fair” during the most recent study period (2021- 2022). Futch Creek has maintained a “Fair” rating for nine (9) of the fourteen (14) years, however improved to "Good" during the past two (2) study periods. Pages Creek has demonstrated varying dissolved oxygen levels over time ranging from “Poor” to “Good” over the years and has been deemed “Fair” during five (5) of the past six (6) study periods. Prince George Creek has demonstrated the worst long-term dissolved oxygen levels compared to the other creeks in the study as it has been designated as “Poor” twelve (12) of the fourteen (14) years. It should be noted that the slow-moving water and swamp- like features within portions of Prince George Creek may help naturally facilitate these low dissolved oxygen levels. Biological Parameter Discussion While several creeks have exhibited relatively low levels of bacteria throughout the lifetime of the program (namely Futch Creek and Lords Creek), other creeks have proven to show elevated levels of Enterococci. Of the 3,189 samples collected and analyzed from all of the monitoring sites since June 2008, 722 samples (23% of all samples) have exceeded the State standard for this bacterium. Specifically, Mott CPE-NC 14 Creek has exceeded the standard 39% of the time and PC-BDDS and PC-BDUS within Pages Creek have exceeded the standard 41% and 62% of the time, respectively. During this year's study period (2021-2022), the collective bacteria levels within the creeks were observed to be similar to the previous year’s effort with the exception of Mott Creek which saw an improvement in rating from “Fair” to “Good”. In general, the past two years represent an overall improvement in water quality with respect to Enterococci compared to the preceding study periods. No samples collected from within four creeks (Lords Creek, Futch Creek, Barnard Creek, and Island Creek) exceeded the State Enterococci standard during this most recent study period. At Smith Creek, Mott Creek, and Prince George Creek, the standard was exceeded 7%, 8%, and 11% of the time during this time, respectively. Pages Creek continued to show elevated levels of Enterococci where eleven (11) out of 36 samples (31%) exceeded the State standard. None of the samples collected from the site at the mouth of Pages Creek (PC-M) exceeded the standard. Of the eleven (11) samples that exceeded the standard, six (6) were from the Pages Creek down-stream site (PC-BDDS) and five (5) were from the up-stream sampling site (PC-BDUS). Of the twenty (20) sites included in this program, the site PC-BDUS has exhibited the highest rate of Enterococci above state standards. This station, located at the boat ramp in the Bayshore community within the Pages Creek watershed, has exceeded the standard 62% of the time since sampling began in 2008. In 2008 and 2013 source tracking studies was performed identifying a human signature in the bacteria that was present in the waters at the PC-BDUS site within Pages Creek. In coordination, the New Hanover County Health Department, and the Cape Fear Public Utility Authority, investigated abandon septic systems and conducted inspections of sewer infrastructure to determine if those were a contributing factor to the elevated bacteria levels. These investigations did not reveal any deficiencies. More recently in 2019, New Hanover County partnered with the University of North Carolina Wilmington’s Socio- Environmental Analysis Laboratory and Coastal Protection Engineering of North Carolina to conduct a thermal imagery scan of two portions of Pages Creek in an attempt identify any possible sources of bacteria entering the creek. In June and July of 2022, CPE performed additional source tracking efforts within two areas where the thermal imagery showed temperature differences indicating the presence of point-source effluent entering the creek. These areas were characterized as groundwater seeps located along the creek’s bank and visualized only at lower tides. Both areas were in proximity to the long-term monitoring sampling sites in the Bayshore neighborhood (PC-BDUS and PC-BDDS) and directly adjacent to CFPUA lift stations. Water samples were collected from these areas on June 13 and July 11, 2022 and were subsequently analyzed by a commercial laboratory using the most widely applied method for the characterization of human fecal pollution in ambient surface waters: Quantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genus Bacteroides (Green et al., 2014). A large number of laboratory and field studies have shown that analyses of host-associated molecular marker genes such as HF183 can identify sources of fecal contamination in waterways with a high degree of precision. Accordingly, this tool has become an established indicator for human sewage contamination in temperate environments (Nshimyimana et al., 2014). Both samples collected in proximity to PC-BDUS resulted in a “Non-Detect” where the host-associated fecal gene biomarker (HF183) were not detected in one or both test replicates. The two samples collected from the seep located in proximity to PC-BDDS resulted in “Detected, Not Quantified” where the host-associated fecal biomarker was detected in both test replicates but in quantities below the limit of quantification. CPE-NC 15 At Mott Creek, the bacteria levels continue to improve; similar to the results observed during the past three (3) study periods. During 2021-2022, only two (2) samples were above the state standard while during the three previous sampling periods , three (3), six (6), and six (6) samples above the standard were collected, respectively. Prior to the 2016-2017 sampling effort, Mott Creek had consistently demonstrated “Poor” water quality in terms of bacterial contamination. As mentioned above, part of this improvement comes from the result of transitioning residential homes with failing septic systems to the CFPUA’s sewer system in the Marquis Hills community (located within the Mott Creek watershed). Since that time the data has reflected, that bacteria levels have reduced proving the need for additional septic to sewer conversions in other watersheds. CFPUA has planned upgrades to the wastewater infrastructure which may further improve overall water quality in the watershed. New Hanover County has also completed a drainage project via its Stormwater Services Program to improve flow. Data has shown reduced levels of bacteria and improvements in dissolved oxygen and chlorophyl-a since the completion of that project in 2019. Overall Watershed Rating The long-term trends for Enterococci ratings over the past fourteen (14) years have shown that Mott Creek, Pages Creek, and Prince George Creek maintained “Poor” ratings during much of the time; however, over the past three (3) years these three creeks have demonstrated some improvements. Mott Creek, which was deemed “Poor” between 2008 and 2016, has improved to “Good” for the first time this year and was deemed “Fair” over the previous five (5) study periods. Similarly, Smith Creek has improved over recent years as it has been rated as "Good" for Enterococci levels over the past four (4) years in a row whereas previous years it contained either "Poor" or "Fair" levels. Barnards Creek, Lords Creek, and Prince George Creek have demonstrated varying conditions since sampling was first initiated. Futch Creek, meanwhile, has consistently maintained a “Good” rating, with two exceptions when it was deemed “Fair”. CPE-NC 16 Airlie Gardens Discussion The results from monthly sampling over the past seven (7) years have provided some insight into the water quality within the lake. There are no State or federal standards for nutrients including the two monitored within Airlie Gardens (orthophosphate and nitrate/nitrite). That said, the levels of orthophosphate and nitrate/nitrite observed within the three (3) sites in Airlie Gardens were generally low. However, generally speaking, since 2015-2016 at the AG-IN site, nitrate/nitrite levels have been relatively higher on average compared to the other two sites further south and closer to the outfall and orthophosphate has been steadily increasing over time across all sites. Over the past seven (7) years of sampling, the orthophosphate level within all three sampling sites have averaged 0.06mg/l. Nitrite/Nitrate levels have been 0.06 mg/l at AG-IN while AG-FD and AG-OUT averaged 0.03 mg/l. This suggests that the nutrient-rich stormwater runoff delivered to the lake at AG-IN are ultimately taken up by aquatic plants and macroalgae within the lake. Phosphorus is a particularly vital nutrient for converting sunlight into usable energy, and essential to cellular growth and reproduction. Under natural conditions phosphorus is typically scarce in water. In the late 1960s scientists discovered phosphorus contributed by human activity to be a major cause of excessive algae growth and degraded lake water quality (MPCA, 2008). The process involving an increase of nutrient loading to a waterbody, called eutrophication, can lead to algae blooms. As the vegetation dies off and the plant matter decomposes, bacteria take up the oxygen in the water column, which can be harmful to fish and other aquatic life. To help combat problems associated with this eutrophication and overall water quality, Airlie Gardens has implemented initiatives identified in their stormwater master plan. These initiatives include installing several aerators in the lake to increase the dissolved oxygen levels. In addition, the tributary that delivers stormwater runoff into the lake just upstream from the AG-IN sampling location was restored in early 2019 which included the planting of native Cypress and the installation of an engineered wetland BMP. In 2020 the County completed a dredging operation by excavating 5' deep by 10' wide channels in the lake, effectively removing approximately 4,000 cubic yards of bottom sediment and material. The removal of the nutrient-laden sediments will ideally result in decreased levels of orthophosphate and nitrate/nitrite within the water column which should result in a reduction of algal blooms thereby helping to maintain appropriate levels of dissolved oxygen. Since these implementation projects were recently completed, the monitoring data has not shown a reduction on nutrient loading into the lake, but it may take a couple to several years to see a trend emerge. It is recommended to continue to monitor the lake to gauge the status of the lake and the effectiveness of the implemented projects. CPE-NC 17 APPENDIX A: ADDITIONAL CREEK DATA Barnards Creek Sampling was conducted at one site (BC-CBR) within the Barnards Creek watershed (Figure 2). Dissolved oxygen within BC-CBR ranged between 1.1 mg/l and 10.0 mg/l with a mean value of 5.5 mg/l (Table 1). Two (2) samples contained dissolved oxygen levels below the State standard of 4.0 mg/l for C Sw waters at the surface (Figure 3). Chlorophyll-a ranged between 1.0 ug/l and 5.0 ug/l with a mean value of 2.0 ug/l at BC-CBR (Table 1). These values did not approach the 40 ug/l standard. Enterococci ranged between 5 CFU/100 ml and 63 CFU/100 ml with a geometric mean value of 21 CFU/100 ml, which is below the NCDEQ standard of 500 CFU/100 ml for Tier III waters (Table 1). None of the twelve (12) samples collected during this period exceeded this standard. Turbidity values were generally good, ranging between 2 and 18 NTU with a mean value of 6 NTU (Table 1). No samples exceeded the State standard of 50 NTU for C SW waters. Table 2 depicts the ratings for these parameters for the watershed. Table 1. Mean values of select parameters from Barnards Creek. Range in parentheses. Figure 2. Water Quality Sites within the Barnards Creek Watershed CPE-NC 18 Parameter BC-CBR Turbidity (NTU) 6 (2-18) Dissolved Oxygen (mg/l) 5.1 (1.1-10.0) Chlorophyll-a (ug/l) 2 (1-5) Enterococci (#CFU/100ml) 21 (5-63) (1) Enterococci values expressed as geometric mean Figure 3. Dissolved Oxygen at BC-CBR at surface (DO-S) and bottom (DO-B) Figure 4. Enterococci at BC-CB CPE-NC 19 Table 2. Ratings of parameters within sampling stations within Barnards Creek Parameter BC-CBR Turbidity GOOD Dissolved Oxygen GOOD Chlorophyll-a GOOD Enterococci GOOD CPE-NC 20 Futch Creek Sampling was conducted at four (4) sites (FC-4, FC-6, FC-13, and FC-FOY) within the Futch Creek watershed (Figure 5). Dissolved oxygen within Futch Creek ranged between 4.4mg/l and 9.9 mg/l with a mean value of 7.5 mg/l (Figure 6 – Figure 9, Table 3). Two (2) samples contained dissolved oxygen levels below the State standard of 5.0 mg/l for SA water Chlorophyll-a ranged between 0.0 ug/l and 13.0 ug/l with a mean value of 3.0 ug/l (Table 3). None of these values approached the 40ug/l chlorophyll-a standard. Enterococci ranged between 1 CFU/100ml and 112 CFU/100ml with a geometric mean value of 7 CFU/100ml. No samples collected within Futch Creek during exceeded the NCDEQ Enterococci standard of 500 CFU/100 ml for Tier III waters (Figure 10 – Figure 13, Table 3). Turbidity values were generally low ranging between 0 and 15 NTU with a mean value of 4 NTU (Table 3). No samples exceeded the State standard of 25 NTU for SA waters during this study period. Table 4 depicts the ratings for these parameters for the watershed. Figure 5. Water Quality Sites within the Futch Creek Watershed CPE-NC 21 Table 3. Mean values of select parameters from Futch Creek. Range in parentheses. Parameter FC-4 FC-6 FC-13 FC-FOY Turbidity (NTU) 3 (1-9) 4 (0-9) 6 (0-12) 5 (0-15) Dissolved Oxygen (mg/l) 7.8 (5.7-9.6) 7.6 (5.2-9.9) 7.2 (4.4-9.7) 7.5 (5.1-9.9) Chlorophyll-a (ug/l) 2 (0-5) 2 (0-5) 3 (1-13) 3 (0-12) Enterococci (#CFU/100ml) 6 (1-81)1 6 (1-101)1 8 (1-112)1 8 (1-92)1 (1)Enterococci values expressed as geometric mean Figure 6. Dissolved Oxygen at FC-4 at surface (DO-S) and bottom (DO-B) Figure 7. Dissolved Oxygen at FC-6 at surface (DO-S) and bottom (DO-B) CPE-NC 22 Figure 8. Dissolved Oxygen at FC-13 at surface (DO-S) and bottom (DO-B) Figure 9. Dissolved Oxygen at FC-FOY at surface (DO-S) and bottom (DO-B) Figure 10. Enterococci at FC-4 CPE-NC 23 Figure 11. Enterococci at FC-6 Figure 12. Enterococci at FC-13 Figure 13. Enterococci at FC-FOY Table 4. Ratings of parameters within sampling stations within Futch Creek CPE-NC 24 Parameter FC-4 FC-6 FC-13 FC-FOY Turbidity GOOD GOOD GOOD GOOD Dissolved Oxygen GOOD GOOD FAIR GOOD Chlorophyll-a GOOD GOOD GOOD GOOD Enterococci GOOD GOOD GOOD GOOD CPE-NC 25 Island Creek Sampling was conducted at one (1) site (IC-HS) within the Island Creek watershed (Figure 14). Dissolved oxygen at IC-HS ranged between 2.4 mg/l and 10.2 mg/l with a mean value of 5.6 mg/l (Table 5). Three (3) samples were below the State standard of 4.0 mg/l for C Sw waters during the sampling period (Figure 15). Chlorophyll-a ranged between 1 ug/l and 9 ug/l with a mean value of 4 ug/l (Table 5). No samples exceeded the State standard of 40 ug/l for chlorophyll-a. Enterococci ranged between 5 CFU/100ml and 305 CFU/100ml with a geometric mean value of 30 CFU/100ml (Table 5). None of the twelve (12) samples collected over this reporting period contained high levels of Enterococci beyond the NCDEQ standard of 500 CFU/100 ml for Tier III waters. Turbidity values were generally moderate ranging between 2 and 8 NTU with a mean value of 4 NTU (Table 5). No samples exceeded the State standard of 50 NTU for C Sw waters in Island Creek during the reporting period. Table 6 depicts the ratings for these parameters for the watershed Figure 14. Water Quality Site within the Island Creek Watershed CPE-NC 26 Table 5. Mean values of select parameters from Island Creek. Range in parentheses. Parameter IC-HS Turbidity (NTU) 4 (2-8) Dissolved Oxygen (mg/l) 5.6 (2.4-10.2) Chlorophyll-a (ug/l) 4 (1-9) Enterococci (#CFU/100ml) 30 (5-305)1 (1)Enterococci values expressed as geometric mean Figure 15. Dissolved Oxygen at IC-HS at surface (DO-S) and bottom (DO-B) Figure 16. Enterococci at IC-HS Table 6. Ratings of parameters within sampling stations within Island Creek Parameter IC-HS Turbidity GOOD Dissolved Oxygen FAIR Chlorophyll-a GOOD Enterococci GOOD CPE-NC 27 Lords Creek Sampling was conducted at one (1) site (LC-RR) within the Lords Creek watershed (Figure 17). Dissolved oxygen at LC-RR ranged between 4.5 mg/l and 10.9 mg/l with a mean value of 7.8 mg/l (Table 7). No samples were below the State standard of 4.0 mg/l for C Sw waters during the sampling period (Figure 18). Chlorophyll-a ranged between 3 ug/l and 39 ug/l with a mean value of 14 ug/l (Table 7). No samples exceeded the State standard of 40 ug/l for chlorophyll-a. Enterococci ranged between 5 CFU/100ml and 52 CFU/100ml with a geometric mean value of 15 CFU/100ml (Table 7). None of the twelve (12) samples collected over this reporting period contained high levels of Enterococci beyond the NCDEQ standard of 500 CFU/100 ml for Tier III waters (Figure 19). Turbidity values were generally moderate ranging between 3 and 21 NTU with a mean value of 10 NTU (Table 7). No samples exceeded the State standard of 50 NTU for C Sw waters in Lords Creek during the reporting period. Table 8 depicts the ratings for these parameters for the watershed. Figure 17. Water Quality Site within the Lords Creek Watershed CPE-NC 28 Table 7. Mean values of select parameters from Lords Creek. Range in parentheses. Parameter LC-RR Turbidity (NTU) 10 (3-21) Dissolved Oxygen (mg/l) 7.8 (4.5-10.9) Chlorophyll-a (ug/l) 14 (3-39) Enterococci (#CFU/100ml) 15 (5-52)1 (1)Enterococci values expressed as geometric mean Figure 18. Dissolved Oxygen at LC-RR at surface (DO-S) and bottom (DO-B) Figure 19. Enterococci Levels at LC-RR Table 8. Ratings of parameters within sampling stations within Lords Creek Parameter LC-RR Turbidity GOOD Dissolved Oxygen GOOD Chlorophyll-a GOOD Enterococci GOOD CPE-NC 29 Mott Creek Sampling was conducted at two (2) sites (MOT-CBR, MOT-ND) within the Mott Creek watershed (Figure 20). Dissolved oxygen within Mott Creek ranged between 3.0 mg/l and 10.6 mg/l with a mean value of 6.8 mg/l (Figure 21 and Figure 22, Table 9). One (1) sample collected during the reporting period contained dissolved oxygen levels below the standard (Figure 21 and Figure 22). Chlorophyll-a ranged between 1 ug/l and 110 ug/l with a mean value of 11 ug/l (Table 9). One sample exceeded the 40ug/l standard. Enterococci ranged between 5 CFU/100ml and 2,310 CFU/100ml with a geometric mean value of 89 CFU/100 ml (Table 9). Samples exceeded the NCDEQ standard of 500 CFU/100 ml for Tier III waters during two (2) sampling events during the reporting period (Figure 23 and Figure 24). Turbidity values were generally good ranging between 2 and 23 NTU with a mean value of 8 NTU (Table 7). No turbidity observations exceeded the State standard of 50 NTU for C Sw waters. Table 10 depicts the ratings for these parameters for the watershed. Figure 20. Water Quality Sites within the Mott Creek Watershed CPE-NC 30 Table 9. Mean values of select parameters from Mott Creek. Range in parentheses. Parameter MOT-CBR MOT-ND Turbidity (NTU) 7 (2-23) 10 (5-19) Dissolved Oxygen (mg/l) 6.2 (3.0-10.6) 7.4 (5.6-10.6) Chlorophyll-a (ug/l) 16 (1-110) 6 (2-13) Enterococci (#CFU/100ml) 43 (5-211)1 183 (5-2,310)1 (1)Enterococci values expressed as geometric mean Figure 21. Dissolved Oxygen at MOT-CBR at surface (DO-S) Figure 22. Dissolved Oxygen at MOT-ND at surface (DO-S) CPE-NC 31 Figure 23. Enterococci at MOT-CBR Figure 24. Enterococci at MOT-ND Table 10. Ratings of parameters within sampling stations within Mott Creek Parameter MOT-CBR MOT-ND Turbidity GOOD GOOD Dissolved Oxygen GOOD GOOD Chlorophyll-a GOOD GOOD Enterococci GOOD FAIR CPE-NC 32 Pages Creek Sampling was conducted at three (3) sites (PC-BDDS, PC-BDUS, and PC-M) within the Pages Creek watershed (Figure 25). Dissolved oxygen within Pages Creek ranged between 2.8 mg/l and 12.3 mg/l with a mean value of 7.5 mg/l (Figures 26 - 28, Table 11). Over the twelve (12) month study period, the dissolved oxygen levels were below the State standard four (4) times at PC-BDDS. Dissolved oxygen was within the standard at PC-BDUS and PC-M during all twelve (12) sampling events. Chlorophyll-a ranged between 1 ug/l and 80 ug/l with a mean value of 10 ug/l (Table 11). One (1) sample exceeded the State standard of 40 ug/l for chlorophyll-a. Enterococci ranged between 3 CFU/100 ml and 763 CFU/100 ml with a geometric mean value of 78 CFU/100 ml (Figures 29 – 31, Table 11). Six (6) samples from PC-BDDS and five (5) samples from PC-BDUS, respectively, contained levels higher than the NCDEQ standard. Turbidity values were generally good ranging between 0 and 17 NTU with a mean value of 6 NTU (Table 11). None of the observed turbidity values exceeded the State standard of 25 NTU for class SA waters. Table 12 depicts the ratings for these parameters for the watershed. Figure 25. Water Quality Sites within the Pages Creek Watershed CPE-NC 33 Table 11. Mean values of select parameters from Pages Creek. Range in parentheses. Parameter PC-BDDS PC-BDUS PC-M Turbidity (NTU) 7 (1-17) 7 (3-11) 4 (0-12) Dissolved Oxygen (mg/l) 8.9 (5.6-12.3) 6.2 (2.8-9.5) 7.5 (6.0-9.5) Chlorophyll-a (ug/l) 17 (2-80) 10 (2-37) 3 (1-5) Enterococci (#CFU/100ml) 471 (134-763)1 143 (25-620)1 7 (3-67)1 (1)Enterococci values expressed as geometric mean Figure 26 Dissolved Oxygen at PC-BDDS at surface (DO-S) Figure 27. Dissolved Oxygen at PC-BDUS at surface (DO-S) CPE-NC 34 Figure 28. Dissolved Oxygen at PC-M at surface (DO-S) and bottom (DO-B) Figure 29. Enterococci at PC-BDDS Figure 30. Enterococci at PC-BDUS CPE-NC 35 Figure 31. Enterococci at PC-M Table 12. Ratings of parameters within sampling stations within Pages Creek Parameter PC-BDDS PC-BDUS PC-M Turbidity GOOD GOOD GOOD Dissolved Oxygen FAIR GOOD GOOD Chlorophyll-a GOOD GOOD GOOD Enterococci POOR POOR GOOD CPE-NC 36 Prince George Sampling was conducted at three (3) sites (PG-CH, PG-ML, and PG-NC) within the Prince George Creek watershed (Figure 31). Dissolved oxygen within Prince George Creek ranged between 0.5 mg/l and 9.8 mg/l with a mean value of 4.9 mg/l (Table 13). Surface dissolved oxygen values were below the State standard of 4.0 mg/l for C Sw on eight (8) occasions during the reporting period at PG-NC, three (3) times at both PG-ML and PG-CH (Figures 33 – 35, Table 13). Chlorophyll-a ranged between 1 ug/l and 28 ug/l with a mean value of 6 ug/l (Table 13). No samples from Prince George Creek exceeded the 40 ug/l standard. Enterococci ranged between 5 CFU/100ml and 3,780 CFU/100ml with a geometric mean value of 56 CFU/100ml (Table 13). Four (4) samples collected from within Prince George Creek contained Enterococci levels above the NCDEQ standard of 500 CFU/100ml for Tier III waters (Figures 36 – 38). Turbidity values were generally good ranging between 1 and 11 NTU with a mean value of 4 NTU (Table 13). No samples exceeded the State standard of 50 NTU for C Sw waters. Table 14 depicts the ratings for these parameters for the watershed. Figure 32. Water Quality Sites within the Prince George Creek Watershed CPE-NC 37 Table 13. Mean values of select parameters from Prince George Creek. Range in parentheses. Parameter PG-CH PG-ML PG-NC Turbidity (NTU) 5 (3-9) 3 (1-8) 5 (1-11) Dissolved Oxygen (mg/l) 5.9 (3.1-9.8) 5.9 (0.5-9.7) 3.0 (0.5-8.0) Chlorophyll-a (ug/l) 7 (2-28) 5 (1-10) 4 (2-7) Enterococci (#CFU/100ml) 79 (5-2,220)1 67 (5-565)1 33 (5-3,780)1 (1)Enterococci values expressed as geometric mean Figure 33. Dissolved Oxygen at PG-CH at surface (DO-S) and bottom (DO-B) Figure 34. Dissolved Oxygen at PG-ML at surface (DO-S) CPE-NC 38 Figure 35. Dissolved Oxygen at PG-NC at surface (DO-S) and bottom (DO-B) Figure 36. Enterococci at PG-CH Figure 37. Enterococci at PG-ML CPE-NC 39 Figure 38. Enterococci at PG-NC Table 14. Ratings of parameters within sampling stations within Prince George Creek Parameter PG-CH PG-ML PG-NC Turbidity GOOD GOOD GOOD Dissolved Oxygen FAIR FAIR POOR Chlorophyll-a GOOD GOOD GOOD Enterococci FAIR GOOD GOOD CPE-NC 40 Smith Creek Sampling was conducted at five (5) sites (SC-CH, SC-23, SC-NK, SC-GR, SC-CD) within the Smith Creek watershed (Figure 39). The sampling site SC-CH was inaccessible between January and June 2022 due to construction of the bridge spanning the creek at Castle Hayne Rd and therefore no data was collected from the site during that timeframe. Dissolved oxygen within the creek ranged between 3.5 mg/l and 10.2 mg/l with a mean value of 7.0 mg/l (Table 15; Figures 40 – Figure 44). Two (2) samples collected were below the State standard. Chlorophyll-a ranged between 0 ug/l and 79 ug/l with a mean value of 6 ug/l (Table 15). One (1) sample exceeded the State Standard for chlorophyll-a from within Smith Creek. Enterococci ranged between 5 CFU/100 ml and 2,780 CFU/100 ml with a geometric mean value of 71 CFU/100ml (Table 15). Three (3) samples exceeded the NCDEQ standard of 500 CFU/100 ml for Tier III waters (Figure 45 -Figure 49). Turbidity values were generally good ranging between 2 and 8 NTU with a mean value of 4 NTU (Table 15). No observations exceeded the State standard of 50 NTU for SW class C waters. Table 16 depicts the ratings for these parameters for the watershed. Figure 39 Water Quality Sites within the Smith Creek Watershed CPE-NC 41 Table 15. Mean values of select parameters from Smith Creek. Range in parentheses. Parameter SC-23 SC-CD SC-CH SC-GR SC-NK Turbidity (NTU) 10 (6-17) 7 (1-19) 12 (7-17) 7 (2-18) 5 (2-9) Dissolved Oxygen (mg/l) 7.0 (3.5-10.2) 8.2 (6.9-10.2) 5.7 (3.7-9.9) 7.4 (4.1-10.0) 6.5 (4.2-10.1) Chlorophyll-a (ug/l) 14 (2-79) 3 (0-9) 4 (1-6) 4 (1-10) 7 (2-15) Enterococci (#CFU/100ml) 34 (5-1,370)1 159 (5-2,780)1 22 (5-75)1 99 (10-414)1 82 (10-548)1 (1)Enterococci values expressed as geometric mean Figure 40. Dissolved Oxygen at SC-23 at surface (DO-S) and bottom (DO-B) Figure 41. Dissolved Oxygen at SC-CD at surface (DO-S) CPE-NC 42 Figure 42. Dissolved Oxygen at SC-CH at surface (DO-S) and bottom (DO-B) Figure 43. Dissolved Oxygen at SC-GR at surface (DO-S) Figure 44. Dissolved Oxygen at SC-NK at surface (DO-S) and bottom (DO-B) CPE-NC 43 Figure 45. Enterococci at SC-23 Figure 46. Enterococci at SC-CD Figure 47. Enterococci at SC-CH CPE-NC 44 Figure 48. Enterococci at SC-GR Figure 49. Enterococci at SC-NK Table 16. Ratings of parameters within sampling stations within Smith Creek Parameter SC-23 SC-CD SC-CH SC-GR SC-NK Turbidity GOOD GOOD GOOD GOOD GOOD Dissolved Oxygen GOOD GOOD GOOD GOOD GOOD Chlorophyll-a GOOD GOOD GOOD GOOD GOOD Enterococci GOOD GOOD GOOD GOOD GOOD CPE-NC 45 Airlie Gardens Airlie Gardens is a 67-acre public garden owned and operated by New Hanover County since 1999. The property, located within the Bradley Creek watershed, and includes a 10-acre freshwater lake. The lake receives input from several stormwater culverts which serves to manage stormwater in the area. Water quality monitoring was conducted at three locations within the lake: AG-IN, located on the northern portion of the lake where stormwater enters the lake; AG-FD, located in a central portion of the lake; and AG-OUT, located at the southern portion of the lake in proximity to the drainage outfall (Figure 50, Table 17). Dissolved oxygen within the lake ranged between 2.1 mg/l and 11.4 mg/l with a mean value of 7.1 mg/l (Table 17; Figures 51 - 53). Three (3) samples were below the State standard for dissolved oxygen. Turbidity values were generally good ranging between 1 and 26 NTU with a mean value of 6 NTU (Table 17). No samples exceeded the State standard of 50 NTU for Class C waters. Chlorophyll-a ranged from 5 mg/l to 135 mg/l with a mean value of 38 mg/l. The standard of 40 mg/l was exceeded twelve (12) times. Figure 50. Airlie Gardens Sampling Sites CPE-NC 46 Figure 51. Dissolved Oxygen at AG-IN Figure 52. Dissolved Oxygen at AG-FD Figure 53. Dissolved Oxygen at AG-OUT CPE-NC 47 Table 17. Mean values of select parameters from Airlie Gardens. Range provided in parentheses. Parameter AG-IN AG-FD AG-OUT Turbidity (NTU) 5 (1-3) 5 (3-11) 8 (3-26) Dissolved Oxygen (mg/l) 5.8 (2.1-9.0) 7.6 (5.1-11.4) 7.8 (4.0-10.8) Chlorophyll-a (mg/l) 28 (5-53) 35 (10-88) 51 (16-135) Orthophosphate 0.11 (0.01-0.42) 0.13 (0.01-0.47) 0.16 (0.01-0.42) Nitrate/Nitrite 0.08 (0.01-0.41) 0.08 (0.01-0.50) 0.07 (0.01-0.26) Long Term Trends within Airlie Gardens Monitoring within three sites in the lake at Airlie Gardens began in the summer of 2015. Since that time, samples have been collected on a monthly basis for the analysis of orthophosphate and nitrate/nitrite (two types of nutrients). In the summer of 2016, monthly samples were collected for the analysis of Chlorophyll-a as well. Over the course of time, some trends have emerged. During the 2016-2017 study period, Chlorophyll-a levels were similar at all three (3) sampling sites, however, the next year (2017-2018 study period) these levels were relatively higher at AG-IN compared to the two (2) sites situated in the central portion of the lake (AG-FD) and at the outfall location (AG-OUT). During the two most recent study periods, however, the trend reversed, and higher levels of Chlorophyll-a was observed at AG-FD and AG- OUT compared to AG-IN (Figure 53). On average, Chlorophyll-a levels at AG-FD and AG-OUT have been considerably higher than the levels observed at AG-IN over time. This may indicate that the nutrients entering the lake from stormwater runoff collected near AG-IN may be taken up by growing vegetation (algae and other aquatic plant matter) as the water flows towards the outfall in proximity to AG-OUT. Figure 54. Chlorophyll-a levels at Airlie Gardens Over Time When examining the levels of nutrients over time within these sampling sites, higher levels of nitrite/nitrate has been observed at AG-IN compared to the other two sampling sites on an annual basis CPE-NC 48 since sampling began in 2015 (Figure 54). Over the past seven (7) years, nitrite/nitrate levels have averaged 0.06 mg/l at AG-IN and 0.03 mg/l at both AG-FD and AG-OUT respectively. This trend of diminishing nutrient levels across the lake (from the stormwater input to the outfall area) held true for orthophosphate as well for the first three (3) sampling periods, however the values have been similar if not increasing slightly across the lake over the past four (4) years (Figure 55). Collectively, over the past seven (7) years of sampling, the average orthophosphate levels have been 0.06 mg/l at each site. Over time, there has been a trend of increasing levels of both nutrients within the lake. During the 2015- 2016 study period, nitrate/nitrite levels averaged 0.03 mg/l and orthophosphate averaged 0.02 mg/l. These levels increased to 0.08 mg/l and 0.14 mg/l, respectively during the 2021-2022 study period (Figure 56). Figure 55. Nitrate/Nitrate Levels in Airlie Gardens Over Time Figure 56. Orthophosphate Levels in Airlie Gardens Over Time CPE-NC 49 Figure 57. Average Nitrite/Nitrate and Orthophosphate Levels in Airlie Gardens Dissolved oxygen within the lake has been good, on average, through the years within each site with the exception of AG-IN during the 2015-2016 study period. The levels at AG-FD and AG-OUT have been similar to each other each year and have been consistently higher in comparison to the levels observed at AG-IN (Figure 57). At each site, the dissolved oxygen levels generally increased during the warmer summer months and increased during the colder winter months. Figure 58. Dissolved Oxygen in Airlie Gardens Over Time CPE-NC 50 APPENDIX B: LONG TERM TRENDS In order to assess the long-term trends in water quality, a database has been created to include the data collected within the eight (7) tidal creeks sampled. Since this is the first-year reporting on parameters of Island Creek, long-term trends have yet to be identified so for the purpose of this section, Island Creek has been omitted. The long-term trends from the seven (7) legacy creeks have been derived from data obtained between July 2008 and June 2022. Dissolved Oxygen Figure 58 depicts the long-term trends in dissolved oxygen within the seven (7) creeks examined within this study. The data show a distinct seasonal pattern including higher dissolved oxygen during the cooler winter months and lower dissolved oxygen during the warmer summer months. Generally speaking, the dissolved oxygen levels within each creek have not changed drastically from year to year. Since 2008, dissolved oxygen levels were below the State standard within surface samples 34%, 22%, 16%, and 10% of the time within Prince George Creek, Pages Creek, Futch Creek, and Barnard Creek, respectively. Dissolved oxygen was below the standard 8%, 6%, and 5% of the time within Mott Creek, Smith Creek, and Lords Creek, respectively. Of the 492 samples that fell below the standard for dissolved oxygen the since 2008, more than half (53%), were observed during June, July, and August when water temperatures were the highest. Figure 59. Long-term surface dissolved oxygen data within tidal creeks. Note: The dissolved oxygen standard within Pages Creek and Futch Creek is 5.0 mg/l while the standard for the other creeks is 4.0 mg/l. CPE-NC 51 Turbidity Figure 59 depicts the long-term trends in turbidity within the seven (7) creeks examined within this study. In general, the long-term trend of turbidity has remained fairly constant within each creek on an annual basis, however several creeks have experienced minor increases over time and seasonal patterns have emerged. This includes higher turbidity observations during the warmer months and lower turbidity during the cooler months. Since 2008, the turbidity standard from observations monitored from the surface waters was only breached nineteen (19) times in total: seven (7) from within Pages Creek and Smith Creek, two (2) from Prince George Creek, and one time each from within Barnards Creek, Lords Creek and Mott Creek. Figure 60. Long-term surface turbidity data within tidal creeks. Note: The turbidity standard within Pages Creek and Futch Creek is 25 NTUs while the standard for the other creeks is 50 NTUs. Chlorophyll-a Figure 60 depicts the long-term trends in chlorophyll-a within the seven (7) creeks examined within this study. In general, the long-term trend of chlorophyll-a has remained fairly constant within each creek. Contrary to the trend observed with dissolved oxygen, chlorophyll-a levels appear to increase during the warmer months and decrease during the cooler months. Since sampling began in July 2008, only 38 exceedances of the chlorophyll-a standard were observed of the 3,189 samples collected. CPE-NC 52 Figure 61. Long-term chlorophyll-a data within tidal creeks Enterococci Figure 59 and Table 16 depict the long-term trends in Enterococci within the seven (7) creeks examined within this study. Of these creeks, Mott Creek, Pages Creek, Smith Creek, and Prince George Creek have maintained relatively higher levels of bacteria over time compared to Lords Creek and Futch Creek. The levels of bacteria in Barnards, Smith, and Mott Creek have moderated over recent years (Table 18). Two sites in particular within the Bayshore community (PC-BDDS and PC-BDUS) in the Pages Creek watershed have demonstrated relatively high levels of Enterococci bacteria over time. Since June 2008, samples collected within Mott Creek and Pages Creek exceeded the State standard for Enterococci 39% and 37% of the time, respectively while Smith Creek and Barnards Creek have exceeded the standard 25% and 24% of the time, respectively and Prince George Creek exceeded standard 23% of the time. Lords Creek exceeded the standard 9% of the time while Futch Creek has only exceeded the standard for Enterococci 4% of the time. CPE-NC 53 Figure 62. Long-term Enterococci data within tidal creeks Table 18. Enterococci ratings for each watershed during all reporting periods. Study Period Barnards Creek Futch Creek Lords Creek Mott Creek Pages Creek Prince George Creek Smith Creek 2008-2009 POOR GOOD FAIR POOR POOR FAIR POOR 2009-2010 POOR GOOD POOR POOR POOR POOR POOR 2010-2011 POOR GOOD GOOD POOR FAIR POOR POOR 2011-2012 POOR GOOD GOOD POOR POOR POOR POOR 2012-2013 POOR GOOD FAIR POOR POOR POOR POOR 2013-2014 GOOD GOOD GOOD POOR POOR POOR FAIR 2014-2015 GOOD GOOD GOOD POOR POOR POOR FAIR 2015-2016 POOR FAIR FAIR POOR POOR POOR FAIR 2016-2017 GOOD GOOD GOOD FAIR POOR GOOD FAIR 2017-2018 FAIR FAIR POOR FAIR POOR POOR POOR 2018-2019 FAIR GOOD FAIR FAIR FAIR GOOD GOOD 2019-2020 GOOD GOOD GOOD FAIR FAIR GOOD GOOD 2020-2021 GOOD GOOD GOOD FAIR POOR FAIR GOOD 2021-2022 GOOD GOOD GOOD GOOD POOR FAIR GOOD CPE-NC 54 APPENDIX C Water Classifications The State of North Carolina has employed a series of classifications that apply to all waters in the State including streams, rivers, and lakes (NC Administrative Code, section 15A NCAC 2B .0200). These classifications are meant to protect the specified uses within waterbodies. These include aquatic life survival and reproduction, secondary recreation, primary recreation, shellfishing, and water supply. The classifications that apply to the creeks examined in this study are: C: Waters: Protected for uses such as secondary recreation, fishing, wildlife, fish consumption, aquatic life including propagation, survival and maintenance of biological integrity, and agriculture. Secondary recreation includes wading, boating, and other uses involving human body contact with water where such activities take place in an infrequent, unorganized, or incidental manner. This includes the lake within Airlie Gardens. C Sw: Freshwater that is protected for aquatic life and secondary recreation uses. The “Sw” supplemental classification indicates that these are swamp waters, and so are likely to have lower dissolved oxygen and pH than non-swamp streams due to natural conditions. However, a majority of the sites, including Lords Creek, Mott Creek, Barnards Creek, Smith Creek, and Prince George Creek, designated as C Sw by the State, are tidally influenced and have a brackish salinity range. SA: Saline water bodies that are protected for shellfishing uses. This use requires a more stringent standard for fecal coliform. Areas protected for shellfishing are also subject to the protection requirements for the less stringent classifications of SC and SB, which include aquatic life, secondary recreation, and primary recreation. This designation applies to Futch Creek and Pages Creek. Parameters Temperature Thermal pollution can result in significant changes to the aquatic environment. Most aquatic organisms are adapted to survive within a specific temperature range. Thermal pollution may also increase the extent to which fish are vulnerable to toxic compounds, parasites, and disease. If temperatures reach extremes of heat or cold, few organisms will survive. Thermal pollution may be caused by stormwater runoff from warm surfaces such as streets and parking lots. Soil erosion is another cause, since it can cause cloudy conditions in a water body. Cloudy water absorbs the sun's rays, resulting in a rise in water temperature. Thermal pollution may even be caused by the removal of trees and vegetation which normally shade the water body. In addition to the direct effects of thermal pollution on aquatic life, there are numerous indirect effects. Thermal pollution results in lowered levels of dissolved oxygen, since cooler water can hold more oxygen than warmer water. Salinity Salinity is a measure of the amount of sodium chloride ions dissolved in water. This is important to monitor since changes in the levels of salt concentration can impact the ability of salt sensitive species to survive. An estuary, such as the lower Cape Fear River, usually exhibits a gradual change in salinity throughout its length, as freshwater entering the estuary from tributaries mixes with seawater moving in from the ocean. Salinity levels control, to a large degree, the types of plants and animals that can live in different zones of the estuary. Freshwater species may be restricted to the upper reaches of the estuary, CPE-NC 55 while marine species inhabit the estuarine mouth. Some species tolerate only intermediate levels of salinity while broadly adapted species can acclimate to any salinity ranging from freshwater to seawater. Conductivity Specific conductance is a measure of the ability of water to conduct an electrical current. Similar to salinity, it measures the amount of dissolved ions (including sodium chloride) in the water. pH The pH of water is a measurement of the concentration of H+ ions, using a scale that ranges from 0 to 14. Natural water usually has a pH between 6.5 and 8.5. While there are natural variations in pH, many pH variations are due to human influences. Unanticipated decreases in pH could be indications of acid rain, runoff from acidic soils, or contamination by agricultural chemicals. Turbidity Turbidity is the amount of particulate matter that is suspended in water. Turbidity measures the scattering effect that suspended solids have on light: the higher the intensity of scattered light, the higher the turbidity. During a rainstorm, particles from the surrounding land are washed into a water body turning the water a muddy brown color, indicating higher turbidity. Dissolved Oxygen Dissolved oxygen (DO) refers to the volume of oxygen that is contained in water. Oxygen enters the water as rooted aquatic plants and algae undergo photosynthesis and as oxygen is transferred across the air- water interface. The amount of oxygen that can be held by the water depends on the water temperature, salinity, and pressure. Rapidly moving water, such as a flowing stream, tends to contain a lot of dissolved oxygen, while stagnant water contains little. Oxygen levels are also affected by the diurnal (daily) cycle. Plants, such as rooted aquatic plants and algae produce excess oxygen during the daylight hours when they are photosynthesizing. During the dark hours they must use oxygen for life processes. Bacteria in water can consume oxygen as organic matter decays. Thus, excess organic material in waterbodies can cause oxygen deficits. Aquatic life can become stressed or die in stagnant water containing high levels of rotting, organic material in it, especially in summer, when dissolved oxygen levels are at a seasonal low. Chlorophyll-a Chlorophyll-a is a green pigment found in plants. It absorbs sunlight and converts it to sugar during photosynthesis. Chlorophyll-a concentrations are an indicator of phytoplankton abundance and biomass in coastal and estuarine waters. High levels often indicate an algal bloom which can induce the depletion of oxygen in the water column due to the microbial degradation of plant cells. Chlorophyll-a concentrations are often higher after rainfall, particularly if the rain has flushed nutrients into the water. Higher chlorophyll-a levels are also common during the summer months when water temperatures and light levels are high because these conditions lead to greater phytoplankton numbers. Enterococci Enterococci are distinguished from fecal coliform bacteria by their ability to survive in saltwater, and in this respect, they more closely mimic many pathogens than do the other indicators. Enterococci are typically more human-specific than the larger fecal streptococcus group. EPA recommends Enterococci as the best indicator of health risk in saltwater used for recreation and as a useful indicator in freshwater as well. In 2004, Enterococci took the place of fecal coliform as the new federal standard for water quality CPE-NC 56 at public beaches. It is believed to provide a higher correlation than fecal coliform with many of the human pathogens often found in sewage (Jeng, et al., 2004). Results indicated that Enterococci might be a more stable indicator than fecal coliform and, consequently, a more conservative indicator under brackish water conditions. Orthophosphate Phosphorus is a nutrient required by all organisms for the basic processes of life. Phosphorus is a natural element found in rocks, soils, and organic material. Phosphorus clings tightly to soil particles and is used by plants, so its concentration in clean waters is generally very low. However, phosphorus is used extensively in fertilizer and other chemicals, so it can be found in higher concentrations in areas of human activity. High levels in the water column can be detrimental to water quality as phosphates can cause algal blooms resulting in decreased dissolved oxygen levels. Orthophosphate is sometimes referred to as "reactive phosphorus." Orthophosphate is the most stable kind of phosphate and is the form used by plants. Orthophosphate is produced by natural processes and is found in sewage. Nitrate/Nitrite Nitrate is highly soluble (dissolves easily) in water and is stable over a wide range of environmental conditions. It is easily transported in streams and groundwater. Nitrates feed plankton (microscopic plants and animals that live in water), aquatic plants, and algae, which are then eaten by fish. Nitrite is relatively short-lived in water because it is quickly converted to nitrate by bacteria. Excessive concentrations of nitrate and/or nitrite can be harmful to humans and wildlife. If excessive amounts of nitrates are added to the water, algae and aquatic plants can be produced in large quantities. When these algae die, bacteria decompose them, and use up oxygen. CPE-NC 57 Standards Water quality standards have been established legislatively for a number of these parameters (Table 19). Many of the water quality standards are described in the NC Administrative Code, section 15A NCAC 2H .0100. The water quality standards for Enterococci bacteria are described by the US EPA (US EPA, 1986) and in the NC Administrative Code, section 15A NCAC 18A .3402. The US EPA standards for Enterococci bacteria are based on incidents of gastrointestinal illness following contact with bathing waters. Bacterial contamination is quantified by “colony forming units” or CFU. Single sample maximum allowable Enterococci density is 104 CFU/100 ml, 158 CFU/100 ml, 276 CFU/100 ml, and 501 CFU/100 ml for designated beach areas, swimming areas with moderate to full body contact, lightly used full body contact swimming areas, and infrequently used full body contact swimming areas, respectively (Table 4). When at least five samples are collected within a 30 day period, the US EPA recommends utilizing a geometric mean standard of 35 CFU/100ml. Geometric means are often useful summaries for highly skewed data, as are often found with bacteriological datasets. The North Carolina Recreational Water Quality Program (RWQ) adopted similar standards for Enterococci bacteria, also determined by the frequency of swimming activity. As defined by RWQ, Tier I swimming areas are used daily during the swimming season, Tier II swimming areas are used three days a week during the swimming season, and Tier III swimming areas are used on average four days a month during the swimming season. Single sample standards for Tiers I, II, and III are 104 CFU/100 ml, 276 CFU/100 ml, and 500 CFU/100 ml, respectively (Table 21). A geometric mean of 35 CFU/100 ml within Tier I swimming areas may also be utilized if at least five samples are collected within 30 days. The creeks and the lake in Airlie Gardens included in this study have not been classified within the RWQ tier system; however, an analysis of accessibility as an indicator of swimming and boating usage has been performed (Table 22). Based on this analysis, of the twenty (20) tidal creek sampling sites, two (2) could be considered Tier II and eighteen (18) could be considered Tier III. All three (3) of the Airlie Garden sites are considered Tier III. Table 19. North Carolina Water Quality Standards Parameter Standard for C Waters Standard for C Sw Waters Standard for SA Waters Dissolved Oxygen 4.0 mg/la 4.0 mg/la 5.0 mg/l Turbidity 50 NTU 50 NTU 25 NTU pH 6.0-9.0b 6.0-9.0b 6.8-8.5 Chlorophyll-a 40.0 ug/l 40.0 ug/l 40.0 ug/l Fecal Coliform Geometric Mean (5 samples within 30 days) <200 CFU/100ml; or single sample <400 CFU/100ml Geometric Mean (5 samples within 30 days) <200 CFU/100ml; or single sample <400 CFU/100ml Geometric Mean (5 samples within 30 days) <14 CFU/100ml; or 10% of samples <43 CFU/100ml Enterococci c Geometric Mean (5 samples within 30 days) <35 CFU/100ml Geometric Mean (5 samples within 30 days) <35 CFU/100ml Geometric Mean (5 samples within 30 days) <35 CFU/100ml (a) Swamp waters may have lower values if caused by natural conditions (b) For swamp streams, pH may be as low as 4.3 if caused by natural conditions (c) See Table 4 for single sample standards based off the tiered system employed by NC DEQ Recreational Water Quality Program Table 20. Single sample standards for Enterococci as determined by the US EPA Description Single sample maximum Designated beach areas < 104 CFU/100 ml CPE-NC 58 Swimming areas with moderate full body contact < 158 CFU/100 ml Lightly used full body contact swimming areas < 276 CFU/100 ml Infrequently used full body contact swimming areas < 501 CFU/100 ml Table 21. Single sample standards for Enterococci as determined by the NC DEQ Recreational Water Quality Program Description Single sample maximum Tier I, swimming areas used daily during the swimming season <104 CFU/100 ml Tier II, swimming areas used three days a week during the swimming season <276 CFU/100 ml Tier III, swimming areas used on average four days a month during the swimming season <500 CFU/100 ml Table 22. Tier Classification for New Hanover County Water Quality Monitoring Sites Site Name Proposed Tier Classification Boating or Swimming Access Comments AG-FD Tier III No Central portion of Airlie Gardens Lake AG-IN Tier III No Northern portion of Airlie Gardens Lake AG-OUT Tier III No Southern portion of Airlie Gardens Lake BC-CBR Tier III No Adjacent to culvert off Carolina Beach Road FC-13 Tier III No Private docks are the only means of direct access FC-4 Tier III No Private docks are the only means of direct access FC-6 Tier III No Private docks are the only means of direct access FC-FOY Tier III No No clear access points (no docks on Foy branch) IC-HS Tier III No Adjacent to culvert off Holly Shelter Road LC-RR Tier III No Adjacent to bridge on River Road MOT-CBR Tier III No Adjacent to culvert off Carolina Beach Road MOT-ND Tier III No Adjacent to small bridge on Normandy Drive PC-BDDS Tier III No Private docks are the only means of direct access PC-BDUS Tier II Yes Public boat ramp off Bayshore Drive PC-M Tier II Yes Direct access via Canady's Yacht Basin Marina PG-CH Tier III No Adjacent to culvert on Castle Hayne Road PG-ML Tier III No Small boat launch site on private property PG-NC Tier III No Adjacent to culvert on North College Road SC-23 Tier III No Adjacent to bridge on 23rd Street SC-CD Tier III No Narrow, shallow. Adjacent to Candlewood Drive SC-CH Tier III No Adjacent to bridge on Castle Hayne Road SC-GR Tier III No Adjacent to culvert on Gordon Road SC-NK Tier III No Adjacent to bridge on North Kerr Table 23. List of Tidal Creek Sampling Sites Creek Name Site Name Site Code Latitude Longitude Barnards Creek Carolina Beach Road BC-CBR 34° 09.522 77° 54.712 Futch Creek 4 FC-4 34° 18.068 77° 44.760 CPE-NC 59 Futch Creek 6 FC-6 34° 18.178 77° 45.038 Futch Creek 13 FC-13 34° 18.214 77° 45.451 Futch Creek Foy Branch FC-FOY 34° 18.405 77° 45.358 Island Creek Holly Shelter IC-HS 34° 22.172 77° 48.544 Lords Creek River Road LC-RR 34° 05.185 77° 55.275 Mott Creek Carolina Beach Road MOT-CBR 34° 08.610 77° 53.830 Mott Creek Normandy Drive MOT-ND 34° 08.373 77° 54.580 Pages Creek Mouth PC-M 34° 16.209 77° 46.270 Pages Creek Bayshore Drive Down Stream PC-BDDS 34° 16.685 77° 47.673 Pages Creek Bayshore Drive Up Stream PC-BDUS 34° 16.623 77° 48.104 Prince George Creek Marathon Landing PG-ML 34° 21.088 77° 55.349 Prince George Creek Castle Hayne Road PG-CH 34° 20.675 77° 54.217 Prince George Creek North College PG-NC 34° 20.331 77° 53.607 Smith Creek Castle Hayne Road SC-CH 34° 15.541 77° 56.325 Smith Creek 23rd Street SC-23 34° 15.472 77° 55.178 Smith Creek Candlewood Drive SC-CD 34° 17.438 77° 51.332 Smith Creek North Kerr SC-NK 34° 15.744 77° 53.256 Smith Creek Gordon Road SC-GR 34° 16.639 77° 52.037 Table 24. List of Airlie Gardens Sampling Sites Site Name Site Code Latitude Longitude Airlie Gardens In AG-IN 34° 21749 77° 82873 Airlie Gardens Floating Dock AG-FD 34° 21549 77° 82796 Airlie Gardens Out AG-OUT 34° 21336 77° 82713 CPE-NC 60 Methods These seven (7) tidal creeks included within this study and the lake in Airlie Gardens are primarily located in the unincorporated portion of New Hanover County. Sampling sites were accessed from land, generally near a bridge or culvert crossing, or by boat. Each tidal creek site was sampled one time per month during a high ebb tide. Tides were determined utilizing the National Oceanic and Atmospheric Administration’s (NOAA) Tides and Currents website (http://tidesandcurrents.noaa.gov/). The sites sampled within Airlie Gardens are not influenced by the tide and therefore no efforts were made to associate the timing of sampling with the tidal stage in the surrounding waters. Due to time constraints, monthly sampling events were conducted on three subsequent days each month. Sites within Airlie Gardens, Lords Creek, Mott Creek, and Barnards Creek were visited on the first sampling day while Smith Creek and Prince George Creek were visited the second day. Futch Creek and Pages Creek were visited on the third day. Rainfall totals for the 24 hours prior to each sampling event were obtained from observations recorded at Wilmington International Airport as reported by NOAA’s National Weather Service web site (http://www.srh.noaa.gov/data/RAH/RTPRAH). Physical Parameters All physical measurements (temperature, salinity, conductivity, turbidity, dissolved oxygen, and pH) were taken in situ utilizing a 6820 YSI Multiparameter Water Quality Probe linked to a YSI 650 MDS display unit. The YSI Probe was calibrated each day prior to use. Physical measurements were taken from the surface at all sites (depth = 0.1 m) and near the creek bottom at sites with depths greater than 0.5 m. Following each sampling trip, the YSI Probe was post-calibrated following each sampling date to ensure that the physical parameters measured were within an acceptable range. Chemical and Biological Parameters Water samples were obtained for the laboratory analysis of chemical (nitrate/nitrite and orthophosphate) and biological (Enterococci and chlorophyll-a) parameters. These grab samples were collected in sterile bottles during a high ebb tide from the surface at each site (depth = 0.1m). Water samples were placed on ice immediately following collection and were delivered in coolers to Environmental Chemists, Inc. of Wilmington, North Carolina for analysis. All analyses performed by Environmental Chemists, Inc. were conducted utilizing the following standard EPA approved methods: • Orthophosphate: SM 4500E • Nitrate/Nitrite: EPA 353.2 • Chlorophyll-a: SM 10200H • Enterococci: EnterolertE CPE-NC 61 APPENDIX D: PAGES CREEK PCR ANALYSIS Following the thermal imagery study and ground truthing in 2019, Coastal Protection Engineering identified areas where groundwater was seeping into Pages Creek from the creek’s bank. Adjacent to sewer infrastructure, the idea was to sample the water from the seeps to understanding if there was any bacteria being carried by groundwater from the infrastructure into the creek. Two samples of groundwater were taken, one at each pump station site adjacent to the creek. Those sampling results indicated the presence of Enterococci bacteria. After the identification of enterococci bacteria coming from the groundwater seeps, NHC and Coastal Protection Engineering and Cape Fear Public Utility Authority partnered in order to test if the seep water contained a human signature or if the bacteria was from another animal such as a dog. The idea this time was further narrow down geographically the source of bacteria. Four samples were collected from the same seeps located near to the long-term monitoring sampling sites in the Bayshore neighborhood (PC- BDUS and PC-BDDS). Water samples were collected from these areas on June 13 and July 11, 2022 and were subsequently analyzed by a commercial laboratory using the most widely applied method for the characterization of human fecal pollution in ambient surface waters: Quantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genus Bacteroides (Green et al., 2014). A large number of laboratory and field studies have shown that analyses of host- associated molecular marker genes such as HF183 can identify sources of fecal contamination in waterways with a high degree of precision. Accordingly, this tool has become an established indicator for human sewage contamination in temperate environments (Nshimyimana et al., 2014). Both samples collected in proximity to PC-BDUS resulted in a “Non-Detect” where the host-associated fecal gene biomarker (HF183) were not detected in one or both test replicates. The two samples collected from the seep located in proximity to PC-BDDS resulted in “Detected, Not Quantified” where the host-associated fecal biomarker was detected in both test replicates but in quantities below the limit of quantification. CPE-NC 62 LITERATURE C ITED Byappanahalli MN, Shively DA, Nevers MB, Sadowsky MJ, Whitman RL. Growth and survival of Escherichia coli and Enterococci populations in the macro-alga Cladophora (Chlorophyta). 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Using geographic information systems and regression analysis to evaluate relationships between land use and fecal coliform bacterial pollution. Journal of Experimental Marine Biology and Ecology. 298:197-209. Kwak, T.J. and Zedler, J.B. 1997. Food web analysis of southern California coastal wetlands using multiple stable isotopes. Oecologia 110: 262–277. Mallin, M.A., 2010. University of North Carolina at Wilmington, Aquatic Ecologist. Personal communication regarding findings of water samples obtained within PG-NC. Mallin, M.A., Ensign, S.H., McIver, M.R., Shank, G.C., and Fowler, P.K. 2001. Demographic, landscape, and meteorological factors controlling the microbial pollution of coastal waters. Hydrobiologia. 460: 185-193. Mallin, M.A.; Williams, K.E.; Esham, C.E.; and Lowe, P.R., 2000. Effect of human development on bacteriological water quality in coastal watersheds. Ecological Applications 10:1047-1056. Mallin, M.A., Matthew R. McIver, Anna R. Robuck and John D. Barker. 2014. Environmental Quality of Wilmington And New Hanover County Watersheds. CMS Report 15-01. Center for Marine Science University of North Carolina Wilmington. 92pp. Meade, R., Yazyk, T. & Day, T. 1990. Movement and storage of sediment in rivers of the United States and Canada. In: The Geology of North America, Woman, H. & Riggs, S. R. (Eds.). Minnesota Pollution Control Agency, 2008. Nutrients: Phosphorus, Nitrogen Sources, Impact on Water Quality - A General Overview Water Quality/Impaired Waters #3.22 website last accessed August 31, 2020. https://www.pca.state.mn.us/sites/default/files/wq-iw3-22.pdf Mote BL, Turner JW, Lipp EK. Persistence and growth of the fecal indicator bacteria Enterococci in detritus and natural estuarine plankton communities. Appl Environ Microbiol. 2012;78(8):2569–2577 NC Division of Commerce, Labor, Economic Data and Site Information. 2015. Thrive in North Carolina, County Demographics Report. http://accessnc.commerce.state.nc.us/docs/countyProfile/NC/37129.pdf. Last visited July 8, 2015. CPE-NC 63 Nshimyimana JP, Ekklesia E, Shanahan P, Chua LH, Thompson JR. Distribution and abundance of human- specific Bacteroides and relation to traditional indicators in an urban tropical catchment. J Appl Microbiol. 2014 May;116(5):1369-83. doi: 10.1111/jam.12455. Epub 2014 Feb 25. PMID: 24460587; PMCID: PMC4271309. Odum, W.E., Smith, T.J., Hoover, J.K., and McIvor, C.C. 1984. The Ecology of Tidal Freshwater Marshes of the United States East Coast: A Community Profile. U.S. Fish and Wildlife Service FWS/OBS-83/17, 177 pp. Olivieri AW, Boehm AB, Sommers CA, Soller JA, Eisenberg JN, Danielson R. (2007). Development of a protocol for risk assessment of separate stormwater system microorganisms. Alexandria: Water Environment Research Foundation. Ricks, C., 2011. Cape Fear Public Utility Authority. Personal communication regarding sewage spills in New Hanover County. U.S. Census Bureau, 2019. Quick facts: New Hanover County, NC. https://www.census.gov/quickfacts/fact/table/newhanovercountynorthcarolina U.S. Environmental Protection Agency. 1984. Health effects criteria for fresh recreational waters. EPA- 600/1-84-004, U.S. Environmental Protection Agency, Washington, D.C. U.S. Environmental Protection Agency. 1986. Ambient Water Quality Criteria for Bacteria- 1986. EPA- 440/5/84-002, U.S. Environmental Protection Agency, Washington, D.C.